[Effect of miR-199a regulating IGF1 expression on differentiation of osteoblasts under mechanical stimulation].

Purpose: To investigate the expression change of microRNA (miR) - 199a in MC3T3-E1 cells stimulated by mechanical stretch and its mechanism of osteogenic differentiation.

Methods: MC3T3-E1 cells cultured in vitro were loaded with 12% stretch for 0, 3, 6, 12 and 24 hours, alkaline phosphatase (ALP) activity was detected by ALP activity test kit, the expressions of osteocalcin (OCN), osteoblast specific transcription factor osterix (OSX), Runt related transcription factor 2 (Runx2) mRNA and miR-199a were detected by real-time fluorescence quantitative PCR. MC3T3-E1 cells were divided into control group, stretch group, stretch + miR-NC group and stretch + miR-199a group, and the expressions of miR-199a, OCN, OSX, Runx2 mRNA and protein and ALP activity were observed after 12% stretch and transfection of miR-199a.

A prediction model for blood-brain barrier permeation and analysis on its parameter biologically.

The objective of this paper is to build a reliable model based on the artificial neural network (ANN) for predicting the blood-brain barrier (BBB) permeability and reveal the effects of the molecular descriptor on the BBB permeability. Eight descriptors including high-affinity P-gp substrate probability and plasma protein binding ratio are selected to develop the model. The three layers feedforward neural network (8-5-1) is employed for the prediction of logBB.