[Preliminary study on molecular detection of polysaccharide from and its intervention to hepatic fibrosis in rats infected with ].

Objective: To detect the molecular characterization of polysaccharide purified from , so as to investigate its role of intervention to the formation of hepatic fibrosis caused by infection.

Methods: The crude polysaccharide from was extracted and further purified, and the molecular weight and monosaccharide composition were determined by the high pressure size exclusion chromatography and PMP pre-column derivatization method, respectively. A total of 50 female BALB/c mice were randomly divided into five groups：A (normal group), B (experimental group), C (polysaccharide group), D (praziquantel), and E (polysaccharide + praziquantel group).

[Experimental study on determination of viability of Schistosoma japonicum cercariae by staining].

Objective: To determine the viability of Schistosoma japonicum cercariae by staining.

Methods: Schistosoma japonicum cercariae were stained by 0.4% trypan blue, 0.

[Preliminary studies on effect of polysaccharides from Tegillarca granosa as an adjuvant on DNA vaccine against Schistosoma japonicum infection].

Objective: To discuss the effect of polysaccharides from Tegillarca granosa as an adjuvant on pcDNA3.1-Sj26GST vaccine against Schistosoma japonicum infection.

Methods: Sixty SPF BALB/c mice were divided into four groups randomly (15 mice each group), including a control group, a polysaccharides group, a vaccine group, and a vaccine plus polysaccharides group.

[Killing effect of exogenous NO on cercariae of Schistosoma japonicum in vitro].

Objective: To explore the killing effects of exogenous NO on the cercariae of Schistosoma japonicum in vitro and the blocking effects of NO inhibitors.

Methods: The cercariae of S. japonicum were collected from naturally infected snails, and then formulated into a 1000 cercariae/ml suspension with RPMI 1640 medium.

[Preliminary studies on protective immunity induced by recombinant protein of Schistosoma japonicum P14 gene and its mechanism against schistosome infection in mice].

Objective: To explore the immune protective effect of the basic calponin-like protein (SjP14) in mice response to the infection of Schistosoma japonicum.

Methods: The recombinant plasmid pET28a(+)-SjP14 was built, and transferred into E. coli BL21 cell line.

[Reform and practice on the experiment teaching of medical parasitology].

A new model of education is investigated to meet the new idea of experiment teaching in university. Therefore the establishment of experiment teaching model of medical parasitology needs to be correspondently reformed. A variety of new management measures are taken to raise the efficiency of experiment teaching in training the students in the College.