[Spatial and Temporal Distribution and Source Variation of Heavy Metals in Cultivated Land Soil of Xiangzhou District Based on EBK Interpolation Prediction and GDM Model].

In order to explore the spatial and temporal changes in spatial patterns and source changes in heavy metals in Xiangzhou District, 395 and 326 soil samples were collected from cultivated soil in Xiangzhou District in November 2009 and November 2019, respectively. The contents of Cr, Pb, As, Hg, and Cd during these two years were measured. The spatial pattern and variation distribution of five types of heavy metals during these two years were obtained by using the empirical Bayesian Kriging (EBK) method.

1,25-dihydroxyvitamin D₃ pretreatment enhances the efficacy of allergen immunotherapy in a mouse allergic asthma model.

Background: Allergen-specific immunotherapy can induce immune tolerance to specific allergens by regulating immune status of individuals. However, its clinical application is limited due to individual differences in efficacy among patients and un-confirmed safety. 1,25 Dihydroxyvitamin D(3) (1,25(OH)(2)D(3)) has been shown to be involved in a variety of physiological processes, including immune response regulation.

QSAR study on MHC class I a alleles based on the novel parameters of amino acids.

MHC-epitope binding plays a key role in the cellular immune response. Accurate prediction of MHC-epitope binding affinity can greatly expedite epitope screening by reducing costs and experimental effort. In this paper, 13 T descriptors， which derived from 544 physicochemical properties of the natural amino acids, were used to characterize 4 MHC class I alleles epitope peptide sequences, the optimal QSAR models were constructed by using stepwise regression combines with multiple linear regression (STR-MLR).

Quantitative sequence-kinetics relationship in antigen-antibody interaction kinetics based on a set of descriptors [corrected].

The interaction between recombinant Fab57P and the coat protein of tobacco mosaic virus was studied using quantitative structure-activity relationship (QSAR) method. The development of quantitative multivariate model has shown to be a promising approach for unraveling protein-protein interactions by designed mutations in peptide sequence. This approach makes it possible to stereo-chemically determine which residue properties contribute most to the interaction.

[Insertion of TC motif into hepatitis B virus core protein c/e1 site does not affect the expression of S and e antigen].

Objective: To investigate whether insertion of TC motif into hepatitis B virus (HBV) core protein c/e1 site affects the expression of S and e antigen.

Methods: Different oligonucleotides encoding TC motif were inserted into the c/e1 site of the core gene of a 1.3 copy wild-type HBV genome vector.

Effects of two novel nucleoside analogues on different hepatitis B virus promoters.

Aim: To explore the effects of the nucleoside analogues beta-L-D4A and beta-LPA on hepatitis B virus (HBV) promoters.

Methods: Four HBV promoters were amplified by polymerase chain reaction (PCR) and subcloned into the expression vector pEGFP-1. The four recombinants controlled by HBV promoters were confirmed by restriction analysis and sequencing.

[Effects of immunotherapy on the expression of costimulatory molecules on dendritic cells in a mouse model of asthma].

Objective: To establish a mouse model of ovalbium in (OVA) specific immunotherapy, and to explore the effects of specific immunotherapy on the expression of CD(80) and CD(86) on spleen-derived dendritic cells (DC) in a mouse asthmatic model.

Methods: A hundred and twenty BALB/c mice were randomly divided into three groups, with 40 mice each. The asthma model (group A) was established by intraperitoneal injection of 10 microg OVA and 1% OVA aerosol challenges.

[Immunological efficiency of DNA vaccine targeting dendritic cells against tumor].

Aim: To construct the DNA vaccine containing ovalbumin (OVA) and Fc fusion gene targeting dentritic cells (DCs) and evaluate its anti-tumor efficiency in an E.G7-OVA-bearing tumor model.

Methods: Constructed OVA-Fc-pcDNA3.

[Exploration of mechanisms underlying T-cell anergy induced by immunotherapy in allergic murine asthma model].

Aim: To explore the mechanisms underlying the T cell anergy induced by immunotherapy for the treatment of allergic asthma.

Methods: Murine model of allergic asthma was established through the OVA sensitization and challenge. Three injections of 1 mg OVA were used as immunotherapy.

[In vitro induction of immune response by dendritic cells pulsed with TRAG-3-derived cytotoxic T lymphocyte epitope].

Objective: To explore the in vitro immune response to taxol resistance associated antigen 3 (TRAG-3)-derived cytotoxic T lymphocyte (CTL) epitope-pulsed dendritic cell (DC).

Methods: The HLA-A2.1 restricted CTL epitope of TRAG-3 was previously identified to be a nonameric peptide sequence from 58 to 66 amino acid residues (TRAG-3(58-66)).

[The mechanism and implication of costimulatory molecules in dendritic cells in the development of asthma].

Objective: To exploring the role of costimulatory molecules in the development of asthma and its mechanisms.

Methods: Murine asthma model was established with ovalbumin (OVA) sensitization and challenge, and the model was confirmed by histological analysis of lung tissues, cell numbers and differentiations of bronchoalveolar lavage, serum OVA-specific IgE level and interleukin (IL)-4 and IL-5 production by splenic T cells. The purity of spleen-derived dendritic cells was assayed with fluorescein-actived cell sorter (FACS) by analyzing CD(11c) molecule.