Amino acid deletions at positions 893 and 894 of cytotoxin-associated gene A protein affect gastric epithelial cell interactions.

Background: () persistently colonizes the human gastric mucosa in more than 50% of the global population, leading to various gastroduodenal diseases ranging from chronic gastritis to gastric carcinoma. Cytotoxin-associated gene A (CagA) protein, an important oncoprotein, has highly polymorphic Glu-Pro-Ile-Tyr-Ala segments at the carboxyl terminus, which play crucial roles in pathogenesis. Our previous study revealed a significant association between amino acid deletions at positions 893 and 894 and gastric cancer.

Linezolid-Resistant Enterococcus faecalis of Chicken Origin Harbored Chromosome-Borne and Plasmid-Borne , (D), and Genes.

The aim of this study was to investigate the transferability of acquired linezolid resistance genes and associated mobile genetic elements in an Enterococcus faecalis isolate QZ076, cocarrying , , (D), and genes. MICs were determined by broth microdilution. Whole-genome sequencing (WGS) was performed using the Illumina and Nanopore platforms.

Detection of microRNAs by the combination of Exonuclease-III assisted target recycling amplification and repeated-fishing strategy.

A simple but effective method for the detection of miRNAs was proposed by integrating exonuclease-III assisted target recycling amplification and repeated-fishing strategy. In the proposed method, exonuclease-III assisted target recycling amplification reaction is adopted to produce a large amount of DNA fragments with fluorescence group at its 5' end in the presence of the target miRNA, which are then repeatedly fished out from the reaction mixture by a gold foil modified with a capture probe and transferred into a so-called 'product tube'. The amount of the target miRNA can then be determined from the fluorescence measurement of the solution in the 'product tube'.

Synthesis and antitumor activity of three novel ginsenoside M1 derivatives with 3'-ester modifications.

Ginsenoside M1 (M1) was considered to be the main antitumor component of ginsenoside metabolites in the body. In order to enhance its potency on antitumor effect, three novel M1 3'-ester derivatives (1c, 2c, 3c) were synthesized and evaluated. The yield of these derivatives was between 41% and 69%.

[Analysis of Anthropogenic Reactive Nitrogen Emissions and Its Features on a Prefecture-level City in Fujian Province].

The impacts of reactive nitrogen (Nr) on the environment significantly increase with population and rapid urbanization. In order to study gaseous Nr and Nr loads to waterbodies at the provincial scale, we established anthropogenic emission inventories in prefecture-level cities in Fujian Province and analyzed the changes in Nr emissions for the years 2000, 2005, and 2010. The total Nr emissions were calculated as 538.

Vimentin is important in the neural differentiation of PC12 cells promoted by sialylation.

Sialic acid modification is a kind of post-translational modification. To investigate the regulation effect of sialic acid on neural differentiation, we used CycloManN propanyl perac (CycloManN pro), a metabolic precursor of sialic acid, to treat PC12 cells. We noted that CycloManN pro indeed robustly promoted global sialylation detected by MAL II lectin blot in PC12 cells.

Antimicrobial susceptibility of Streptococcus sp. to quinupristin-dalfopristin in China.

This study aimed to determine the in vitro activity of quinupristin-alfopristin against Streptococcus sp. isolated in China. This agent is not yet available for clinical use, but it has been tested against a high proportion of resistant Staphylococcus aureus strains.

Molecular epidemiological characteristics of Streptococcus pyogenes strains involved in an outbreak of scarlet fever in China, 2011.

Objective: To investigate molecular characterization of streptococcus pyogenes isolates involved in an outbreak of scarlet fever in China in 2011.

Methods: Seventy-four Streptococcal pyogenes involved in an outbreak of scarlet fever were isolated from pediatric patients in the areas with high incidence in China from May to August of 2011. Emm genotyping, pulsed-field gel electrophoresis (PFGE), superantigen (SAg) genes and antimicrobial susceptibility profiling were analyzed for these isolates.

Disordered epigenetic regulation in MLL-related leukemia.

Leukemias bearing rearrangements of chromosome 11q23 are of particular interest due to their unique clinical and biological characteristics. 11q23 abnormalities occur in up to 70 % of infant leukemias, and about 10 % of adult acute myelogenous leukemias (AML). Two major rearrangements of the MLL gene are found in MLL-related leukemia.

The study of BDNF Val66Met polymorphism in Chinese schizophrenic patients.

Accumulating evidence showed that brain-derived neurotrophic factor (BDNF) may be involved in the pathophysiology of schizophrenia. Recent studies have reported that the Val66Met polymorphism of the BDNF gene may be associated with susceptibility for schizophrenia and age of onset of this disease, with mix results. In the present study, the BDNF Val66Met gene polymorphism was examined in 387 inpatients (259 men and 128 women) meeting the DSM-IV criteria for schizophrenia and unrelated 365 healthy controls (255 men and 110 women).

[Multilocus sequence typing of methicillin-resistant Staphylococcus aureus].

Objective: To analyze multilocus sequence typing (MLST) of methicillin-resistant Staphylococcus aureus (MRSA) strains in 2000 and 2005, and get a primary knowledge of MLST Characterization of MRSA.

Methods: Sequence analysis was conducted on seven allelic genes of 29 methicillin-resistant Staphylococcus aureus strains and 2 methicillin-sensitive Staphylococcus aureus (MSSA) strains and the allelic profiles were gained from internet database.

Results: All 12 MRSA strains in 2000 were sequence type (ST) 239 and 10 MRSA strains in 2005 were ST239, while 7 MRSA strains in 2005 were new types, ST5 (41.

Genetic analysis of group A streptococcus isolates recovered during acute glomerulonephritis outbreaks in Guizhou Province of China.

In this study, 68 group A streptococcus (GAS) isolates associated with two outbreaks of acute glomerulonephritis (AGN) in China were analyzed by emm typing. A total of 11 different emm types were identified. Analysis of emm type distribution suggested that AGN outbreaks in two counties were caused by emm60.

[Monosaccharide composition analysis and its content determination of polysaccharides from Rhaponticum uniforum].

Objective: To analyze the monosaccharide composition in the polysaccharides from Rhaponticum uniforum, determine the content of monosaccharide, and provide some references for further research.

Method: The monosaccharide composition was determined by high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Phenol-sulfuric acid method was used for the determination of the content of polysaccharide.

Cross-reactivity of anti-H pylori antibodies with membrane antigens of human erythrocytes.

Aim: To investigate whether anti-H pylori antibodies have cross-reaction with antigens of erythrocyte membrane.

Methods: Blood samples were collected from 14 volunteers (8 positive and 6 negative for H pylori detected by (13)C-urea breath test) of the general population. Erythrocyte membrane proteins of the subjects were examined by Western blot using anti-H pylori serum.

A novel binuclear palladium complex with benzothiazole-2-thiolate: synthesis, crystal structure and interaction with DNA.

The novel Pd(II) complex, [Pd(2)(micro-bzta)(4)].1.5DMSO (where bzta=benzothiazole-2-thiolate) has been synthesized and structurally characterized by element analysis, IR and single-crystal X-ray diffractometry.

Proteome analysis of sorbitol fermentation specific protein in Vibrio cholerae by 2-DE and MS.

Vibrio cholerae can be differentiated into epidemic and non-epidemic strains by sorbitol fermentation speed, but little research has been done on its mechanisms. In this study, we investigated differential protein expression of the two strains in response to sorbitol metabolism. V.

[Sequence analysis on sorbitol fermentation related genes in Vibrio cholerae].

Objective: To Investigate the differences of sorbitol fermentation related genes and optimize molecular analysis method for distinguishing an epidemic with nonepidemic strains of Vibrio cholerae.

Methods: Sequence analysis on four genes of sugar fermentation stimulation protein, periplasmic maltose-binding protein, periplasmic phosphate-binding protein and periplasmic amino acid-binding protein.

Results: In this study, the following data was noticed: for O1 serogroup El Tor biotype V.

[In-vitro activity of rabeprazole, lansoprazole, and esomeprazole against Helicobacter pylori].

Objective: To investigate the antimicrobial activity of Pariet, Tekpron, Nexium, respectively, against Helicobacter pylori (H. pylori) in vitro.

Methods: Antimicrobial effects of these medicines were evaluated through detection of MICs for 3 H.

[Primary analysis on protein maps of Helicobacter pylori strains associated with gastric carcinoma].

Objective: To identify specific proteins of Helicobacter pylori (H. pylori) that associated with gastric carcinoma.

Methods: The whole-cell proteins of H.

[Study on optimization of expression, purification, properties and biological function of recombinant human sBLyS].

The prokaryotic expression plasmid pET-30a(+)/sBLyS was constructed and transformed into E. coli BL21 (lambda DE3). The recombinant protein was found to be highly expressed by the plight of soluble part and inclusion body.

Cloning, Expression, Purification and Activity of the hsBLyS.

The full length cDNA of human B lymphocyte stimulator (hBLyS) was amplified by using PCR method from cDNA library of human placenta. After purifying and sequencing, the DNA fragment of functional domain of hBLyS (hsDNA fragment)was amplified by using nested PCR method from the PCR product. The prokaryotic expression plasmid pET-30a( )/ hBLyS was constructed with recombinant DNA techniques after purifying and identifying the hsDNA fragment.