[Structural Analysis of Salivary Bacterial and Fungal Microbiome in Oral Lichen Planus Patients].

Objective: To explore the differences of oral mycobiome and bacteriome between the healthy controls (H) and oral lichen planus (OLP) patients, and the co-occurrence patterns of the salivary mycobiome and bacteriome and the association with host immunity.

Methods: Saliva samples were collected from clinical OLP patients (=35) and healthy volunteers (=18). Microbiome DNA was extracted for bacterial 16S rRNA genes sequencing and fungal internal transcribed spacer 2 (ITS2) sequencing.

Analysis of Soluble Sugar Content in Minute Quantities of Rice Tissues by GC-MS.

Soluble sugars play key roles in plant growth, development, and adaption to the environment. Characterizing sugar content profiling of plant tissues promotes our understanding of the mechanisms underlying these plant processes. Several technologies have been developed to quantitate soluble sugar content in plant tissues; however, it is difficult with only minute quantities of plant tissues available.

Characterization of the complete mitochondrial genome of (Orthoptera: Tetrigidae) from China and its phylogenetic analysis.

The mitochondrial genome (mitogenome) of the (Orthoptera: Tetrigidae: Tetriginae) was sequenced and annotated. The assembled mitochondrial genome was 14,947 bp, containing 45.8% of A, 15.

Muc-1 promotes migration and invasion of oral squamous cell carcinoma cells via PI3K-Akt signaling.

Muc-1 is a member of the carbohydrate-binding protein family that contributes to neoplastic transformation, tumor survival, angiogenesis, and metastasis. The aim of this study is to investigate the role of muc-1 in human oral squamous cell carcinoma progression. In this study, we tested our hypothesis that muc-1 regulate oral squamous cell carcinoma cells (SCC-9) malignant biological behaviors, and silencing muc-1 reduced SCC-9 cellular colony forming ability, migration and invasion.

TCAB1: a potential target for diagnosis and therapy of head and neck carcinomas.

Background: WRAP53, including α, β and γ isoforms, plays an important role not only in the stability of p53 mRNA, but also in the assembly and trafficking of the telomerase holoenzyme. It has been considered an oncogene and is thought to promote the survival of cancer cells. The aim of this study was to detect the role of TCAB1 (except WRAP53α) in the occurrence and development of head and neck carcinomas.

Elevated levels of triglyceride and triglyceride-rich lipoprotein triglyceride induced by a high-carbohydrate diet is associated with polymorphisms of APOA5-1131T>C and APOC3-482C>T in Chinese healthy young adults.

Background/aims: Changes in lipid profiles have been shown to be associated with diet and apolipoprotein (APO) polymorphisms. Therefore, 2 polymorphisms, i.e.

Associations of the SREBP-1c gene polymorphism with gender-specific changes in serum lipids induced by a high-carbohydrate diet in healthy Chinese youth.

We investigated the possible association between the sterol regulatory element-binding protein-1c gene (SREBP-1c) rs2297508 polymorphism and the changes in lipid profiles in a high-carbohydrate and low-fat (high-CHO/LF) diet in a Chinese population well characterized by a lower incidence of coronary heart disease and a diet featuring higher carbohydrate and lower fat. Fifty-six healthy youth (aged 22.89 ± 1.

A high-carbohydrate diet enhances the adverse effect of the S2 allele of APOC3 SstI polymorphism on the TG/HDL-C ratio only in young Chinese females.

Both genetic background and diet have profound effects on plasma lipid profiles. We hypothesized that a high-carbohydrate (high-CHO) diet may affect the ratios of serum lipids and apolipoproteins (apo) differently in subjects with different genotypes of the SstI polymorphism in the apoCIII gene (APOC3). Fifty-six healthy university students (27 males and 29 females, 22.

[The latest progress in studies of human oral microbiome].

With the successful implementation of Human Genome Project, more and more scientists started to pay attention on the second genome of human body: Microbiome. This paper will briefly introduce the latest developments of the Human Microbiome Project, the human oral microbiome research, and new technologies of microbial gene research.

TaqIB polymorphism in the CETP gene modulates the impact of HC/LF diet on the HDL profile in healthy Chinese young adults.

The aim of this study was to investigate the interactions of genetic variants in the genes of cholesterol ester transfer protein (CETP) and low-density lipoprotein receptor (LDLR) with high carbohydrate and low fat (HC/LF) diet on lipid profiles in a young and healthy Chinese Han population. Fifty-six healthy subjects (22.89±1.

[Discrimination of common oral streptococcus with metabonomics method].

Objective: To evaluate the feasibility of identifying oral streptococcus by comparing their metabolic profiling, and to find a convenient and rapid way to discriminate oral microorganisms.

Methods: The pure cultivation of Streptococcus sanguis ATCC 10556 and Streptococcus sobrinus 6715 (reference strain) from solid culture were respectively inoculated in TPY liquid medium. Then the growth quantity was measured periodically by turbidimetry and the growth curves of the inoculated bacteria were completed.

[Initial study on the discrimination of oral common Actinomycetes with metabonomics method].

Objective: The method of metabonomics based on 1H-nuclear magnetic resonance (1H-NMR) was preliminarily applied to discriminate the oral common Actinomycetes, Actinomyces naeslundii ATCC12104 and Actinomyces israelii ATCC12102.

Methods: Solutions of Actinomyces naeslundii and Actinomyces israelii with same density were made and cultured respectively at BHI liquid culture medium. The concentration of bacteria was determined periodically, and then the growth curves were drawn.

[Preliminary study on the discrimination of putative periodontal pathogens with a metabonomics method].

Objective: To evaluate the feasibility of identifying oral pathogenic bacteria by comparing the metabolic profiling of putative periodontal pathogens and try to find a convenient and rapid way to discriminate oral microorganisms.

Methods: Suspensions of Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum with same density were prepared and cultured respectively at liquid BHI medium. Then the growth quantity was measured periodically through turbidimetry and the growth curves of the inoculated bacteria were completed.

Identification of a novel gene, MSAG, regulated by high levels of glucose and insulin.

Identification and characterization of novel genes involved in derangement of metabolisms of glucose and triglycerides are important in understanding the development of metabolic syndrome (MS) and atherosclerosis. Model rats with certain phenotypes of MS were fed a high-carbohydrate diet. The rat hepatic subtracted cDNA libraries were constructed and screened.

[Therapeutic effects of hTR-siRNA adenovirus on human cervical cancer xenografts in nude mice].

Objective: To study the anti-tumor effects and its mechanism of hTR-siRNA adenovirus on human cervical cancer in vivo.

Methods: The in vivo model of human cervical cancer was established by the subcutaneous inoculation of HeLa cells at the right armpit of BALB/c nu/nu mice. After successful implantation, the mice were randomized into four groups, in which the mice were intratumorally injected with 0.

[Initial study on the discrimination of oral microorganisms with a metabonomics method].

Objective: To establish the spectra of metabolites that coued be employed in identification of oral pathogenic bacteria, and try to find a convenient and rapid way to discriminate oral microorganisms.

Methods: Suspensions of Streptococcus mutans ATCC 25175, Streptococcus sanguis ATCC 10556 and Lactobacillus acidophilus ATCC 4356 with same density were preparecd and cultured respectively at improved TPY liquid culture medium. The growth quantity were measured periodically by a turbidimeter.

[Construction of adenovirus expressing siRNA against hTR and its anti-tumor activity in vitro].

Aim: To study the effect of the hTR-siRNA adenovirus on hTR mRNA gene silence, telomerase activity inhibition and anti-tumor in vitro.

Methods: RNAi adenovirus vector, Ad-hTR-siRNA, and negative control Ad-NT-siRNA were constructed by an improved ligation method. Different tumor cells and liver cell line, HL-7702, were infected with 100 MOI of the recombinant adenoviruses.

[Initial study on discrimination of oral microorganisms with the metabonomics technique].

Objective: To evaluate the feasibility of employing metabonomics method in identification of oral pathogenic bacteria.

Methods: The Streptococcus mutans ATCC25175 and Actinomyces viscosus ATCC15987 were respectively inoculated in same certain culture medium. The growth curves of the inoculated bacteria were drown by turbidimetry.

[Cloning of ureI gene from Helicobacter pylori and its expression in E. coli].

Objective: To clone the urea membrane channel gene (ureI) from Helicobacter pylori (Hp) for its expression in E. coli, and evaluate the expression conditions and immunological features of the fusion protein.

Methods: ureI gene cloned by PCR from Hp was inserted into the plasmid pET32a (+) to construct the recombinant plasmid pET32a/ureI, followed by identification by BglII and HindIII digestion and sequencing.

[Evaluation on cytotoxicity of a new nano-hydroxyapatite as root canal filling sealer].

Objective: To evaluated the cytotoxicity of a new nano-hydroxyapatite (n-HA) root canal sealer.

Methods: In this study, the cytotoxicity was evaluated by the method of MTT assay in vitro, and culture medium F12 as control, three concentrations of the soaking material cultured with mouse osteoblast separately, to test the cell relative growth rate (RGR) of every group.

Results: The toxicity graduation of the n-HA root canal sealer tends to 0 with the culture time increasing.

[Dynamic release of tinidazole from the degradable PLLA-tinidazole blend gel].

Objective: To determine the dynamic release of tinidazole from the degradable poly-lactic acid (PLLA)-tinidazole blend gel (a periodontal local drug delivery) by high-performance liquid chromatography (HPLC) in vitro and to observe the polylactic acid (as a vector of tinidazole) how to affect the release of tinidazole.

Methods: HPLC analysis was conducted. The operating conditions were C18-ODS-A column, water-methanol-acetic acid as mobile phase at a flow rate of 1.