Farnesane sesquiterpenoids from and their anti- activities.

Two new farnesane sesquiterpenoid glycosides, rubusdelosides A and B ( and ), together with five known farnesane sesquiterpenoids (, , and -), and two known meroterpenoids ( and ), were isolated from water extract of the herbs of . Their structures were identified by the comprehensive analyses of physicochemical properties and spectroscopic data (NMR and HR-ESI-MS). Compounds - showed moderate anti- activities.

A new prenylated coumestan from the roots of .

A new prenylated coumestan, campylohirtin A (), along with fifteen phenolic known compounds () and four other known compounds (), was obtained from the 95% ethanol extract of roots of . Their structures were elucidated based on extensive spectroscopic analysis (1 D and 2 D-NMR, MS, UV and IR). antimalarial activities of compounds -, - and were evaluated by -hematin formation inhibition assay.

A New Antimalarial Noreudesmane Sesquiterpenoid from Dobinea delavayi.

One previously undescribed angeloylated noreudesmane sesquiterpenoid, dobinin O (1), along with four known eudesmane sesquiterpenoids (2-5) were isolated from the peeled roots of Dobinea delavayi. Their structures were elucidated by extensive spectroscopic data analyses. In addition, compound 1 exhibited moderate antimalarial activity against Plasmodium yoelii BY265RFP with the inhibition ratio of 17.

[The construction of recombinant mouse interleukin 4 prokaryotic expressing plasmid and the expression and purification of target protein].

Objective: To construct Recombinant Mouse Interleukin 4 prokaryotic expressing plasmid, express it in E. coli strain BL21 (DE3), purify and identify the expressed cytokine.

Methods: The optimized mIL-4 cDNA fragment was cloned into the prokaryotic expressing vector pET-32a (+) to generate pET32/rmIL-4 and transformed into BL21 (DE3) cells.