Substantial Epigenetic Variation Causing Flower Color Chimerism in the Ornamental Tree Revealed by Single Base Resolution Methylome Detection and Transcriptome Sequencing.

Epigenetic changes caused by methylcytosine modification participate in gene regulation and transposable element (TE) repression, resulting in phenotypic variation. Although the effects of DNA methylation and TE repression on flower, fruit, seed coat, and leaf pigmentation have been investigated, little is known about the relationship between methylation and flower color chimerism. In this study, we used a comparative methylomic⁻transcriptomic approach to explore the molecular mechanism responsible for chimeric flowers in "Danban Tiaozhi".

[Risk Assessment of Snail Output Via the Mud Balls of Transplanted Seedlings in a Nursery Stock Park in Middle Region of Zhejiang Province].

Objective: To investigate the distribution of snails in a nursery stock park in the middle region of Zhejiang Province and assess the risk of snail output via the mud balls of transplanted seedlings, to provide scientific data for making strategies for snail control.

Methods: We selected three species of seedlings including Osmanthus fragrans (a large tree), Camellia sasanqua (a small tree), and Purpus privet (a type of shrub) in a nursery stock park in a snail-positive middle region of Zhejiang Province during 2014-2016 to calculate the areas of regions with snails and the density of living snails. In 30 trees of each species, the distribution of snails within the seedlings ground diameter (radius of investigation, 100 cm for Osmanthus fragrans; 30 cm for Camellia Sasanqua and Purpus Privet) and in different soil layers (surface and superficial layers, 0-3 cm; deep layer, 3-10 cm) was assessed.

[Interpretation of Criteria for Diagnosis of Toxoplasmosis].

The criteria for Diagnosis of Toxoplasmosis (WS/T 486-2015)（referred to as the Criteria） was compiled following the Management Measures for Health Criteria and GBT 1.1-2009 Standardization Working Guidelines. The Criteria is composed of six chapters, including the range of application, terms and definitions, diagnostic principle, diagnostic standard, and differential diagnosis.

[Evaluation on molluscicidal effect of chlorosalicylicamide on Oncomelania hupensis in schistosomiaisis endemic areas of eight provinces in China].

Objective: To evaluate the effects of a novel molluscicide, the salt quinoid-2', 5-dichloro-4'-nitrosalicylanilide from niclosamide (LDS), with 10% wettable powder, in main schistosomiasis epidemic areas of China, including Hunan, Jiangxi, Hubei, Anhui, Jiangsu, Sichuan, Yunnan and Zhejiang Province.

Methods: In the immersion test, 6 effective concentrations of 10% LDS were tested respectively: 0.1, 0.

[Quality control assessments of feces examination for schistosomiasis in province-level laboratories of Zhejiang Province].

Objective: To understand and evaluate the quality of feces examination for schistosomiasis in province-level laboratories of Zhejiang Province.

Method: With the single-blind method, the stool samples were detected by the stool hatching method and sediment detection method.

Results: In the 3 quality control assessments in 2006, 2008 and 2009, most laboratories finished the examinations on time.

[Strategy of solidification and surveillance for schistosomiasis in transmission-interrupted provinces in China].

In schistosomiasis transmission-interrupted provinces in China, the endogenous source of infection has been basically cleared, and the exogenous source of infection is considered as a major threat. The residual snail area has been increasing in different degrees, and imported snails are regarded as a potential risk. So the possibility of schistosomiasis recrudescence still remains.

Development of a rapid dipstick with latex immunochromatographic assay (DLIA) for diagnosis of schistosomiasis japonica.

Background: Schistosomiasis japonica (schistosomiasis) is a zoonosis that can seriously affect human health. At present, the immunodiagnostic assays for schistosomiasis detection are time-consuming and require well-trained personnel and special instruments, which can limit their use in the field. Thus, there is a pressing need for a simple and rapid immunoassay to screen patients on a large scale.

[Recent investigation and treatment of 1 060 advanced schistosomiasis cases in Zhejiang Province].

Objective: To understand the current status of advanced schistosomiasis patients, deliver medical treatment, and improve the case management in Zhejiang Province.

Methods: The cases previously diagnosed or suspected as advanced schistosomiasis in the province were the subjects of the investigation. Questionnairing (demographical information, disease history, etc.

[Establishment and application of rapid dipstick of latex immunochromatographic assay for the diagnosis of schistosomiasis].

Objective: To establish a simple and fast diagnostic assay for schistosomiasis.

Methods: Based on the immunochromatographic technique and the principle of indirect assay of ELISA, using soluble egg antigen (SEA) of Schistosoma japonicum and mouse anti-human monoclonal antibody labelled with red latex as color developing agents, a latex immunochromatographic assay (DLIA) was developed. Serum samples from 69 schistosomiasis patients were detected by DLIA.

A randomized, double-blind, multicenter clinical trial on the efficacy of ivermectin against intestinal nematode infections in China.

To assess the efficacy of ivermectin against intestinal nematode infections, a randomized, double-blind, multicenter clinical trial was carried out in a total of 816 human individuals infected with different nematodes from three counties in China. The subjects were randomly assigned into experimental and control groups and orally given a single dose of 0.1, 0.

[Observation on the change of anti-S. japonicum antibody level in population migrated from outside embankment to new town].

Objective: To detect the change of the anti-S. japonicum antibody level after people migrated from outside embankment to newly established town.

Methods: Three pilot spots were established for the investigation: one spot that both inhabitancy and cultivation disuse (A), one spot that only inhabitancy disuse but farming continued (B) and the third one served as control (C).

[Prophylactic effect of artesunate against experimental infection of Schistosoma mansoni].

Objective: To study the prophylactic effect of artesunate against the infection of Schistosoma mansoni in mice and its optimal scheme for preventing schistosomiasis mansoni.

Methods: BALB/c mice were infected by tail dipping method with S. mansoni cercariae.

[Clinical observation on the efficacy of ivermectin in the treatment of intestinal nematode infections].

Objective: To observe the efficacy and side effects of ivermectin in the treatment of intestinal nematode infections.

Methods: A single dose of ivermectin 0.1, 0.