PCSK9 induces endothelial cell autophagy by regulating the PI3K/ATK pathway in atherosclerotic coronary heart disease.

Objective: Atherosclerosis is a chronic inflammatory disease of the arteries, and its pathogenesis is related to endothelial dysfunction. It has been found that the protein convertase subtilin/kexin9 type (PCSK9) plays an important role in AS, but its specific mechanism is still unclear.

Methods: In this study, we first cultured human umbilical vein endothelial cells (HUVECs) with 50 or 100μg/ml oxidized low-density lipoprotein (ox-LDL) for 24 hours to establish a coronary atherosclerosis cell model.

The perilous consequences of bowel preparation: a case study with literature review of Boerhaave syndrome.

Colonoscopy is widely acknowledged as a prevalent and efficacious approach for the diagnosis and treatment of gastrointestinal disorders. In order to guarantee an effective colonoscopy, it is imperative for patients to undergo an optimal bowel preparation regimen. This entails the consumption of a substantial volume of a non-absorbable solution to comprehensively purge the colon of any fecal residue.

Machine-learning-assisted SERS nanosensor platform toward chemical fingerprinting of Baijiu flavors.

A novel fingerprinting platform for multiplex detection of flavor molecules in Baijiu was developed by using a surface-enhanced Raman scattering (SERS) nanosensor array in combination with machine learning. The SERS sensors were constructed by core-shell FeO@Ag nanoparticles modified with molecules carrying end-groups of hydroxyl, pyridyl, methyl, and amino, respectively, which interacted with flavors and led to changes in the sensors' spectra. All the Raman spectra acquired from the nanosensor array contacting with the sample were concatenated into a single SERS super-spectrum, representing the flavor fingerprint which was recognized through machine learning.

Diagnosis of Schizophrenia Based on Deep Learning Using fMRI.

Schizophrenia is a brain disease that frequently occurs in young people. Early diagnosis and treatment can reduce family burdens and reduce social costs. There is no objective evaluation index for schizophrenia.

An all-in-one magnetic SERS nanosensor for ratiometric detection of Escherichia coli in foods.

An all-in-one nanosensor was developed for the magnetic enrichment and ratiometric surface-enhanced Raman scattering (SERS) detection of Escherichia coli (E. coli). The all-in-one nanosensor was constructed through the chemical integration of four components into a single nanoparticle, which include a manganese ferrite nanoparticle serving as the magnetic core, a thin silver shell as the SERS substrate, a self-assembled layer of 4-mercaptobenzoic acid (MBA) molecules as the SERS internal standard, and a MBA-conjugated layer of aptamer sequences as the capture probe of E.

Higher quantum efficiency and moisture resistance of all-inorganic halide perovskite nanocrystal films in situ fabricated with cyclodextrin.

Supermolecule β-cyclodextrin was used to assist CsPbBr3 film fabrication. In situ growth of nanocrystals was effectively confined through strong interactions between perovskite Pb2+ ions and β-CD hydroxyl groups, producing a compact and smooth film. The quantum efficiency achieved was 85.

Curcumin Acetate Nanocrystals for sustained Pulmonary Delivery: Preparation, Characterization and In Vivo Evaluation.

The main objective of this study was to test the hypothesis that inhaled nanocrystals of a highly lipophilic drug could be used as a novel approach for producing sustained pulmonary delivery. Curcumin acetate, an ester prodrug of curcumin, was utilized as a highly lipophilic model drug. Curcumin acetate was subjected to wet ball milling to produce different particle sizes of nanocrystals and microparticles, and the milled curcumin acetate was spray-dried to yield similar inhalable microparticles.

Electrodeposition of polyaniline onto TiO2 nanoparticles/multiwalled carbon nanotubes for visible light photoelectrocatalysis.

In this study, polyaniline (PANI) was coated onto TiO2 nanoparticles/multiwalled carbon nanotubes (TiO2/MWCNTs) hybrid by electrochemical polymerization. Modification of TiO2/MWCNTs with PANI endows the resulted hybrid with visible light activity. The PANI/TiO2/MWCNTs hybrid shows remarkable photoelectrocatalytic activity for the degradation of RhB under visible light irradiation.

Acoustic sensing of the initial adhesion of chemokine-stimulated cancer cells.

Chemokines together with their receptors play important roles in tumor metastasis. Intracellular signals stimulated by chemokines regulate the initial adhesion of cancer cells, which controls the subsequent cell spreading and migration. Until now, the nature of initial cell adhesion has been understood very poorly, since conventional assays are static and could not provide dynamic information.

Oily nanosuspension for long-acting intramuscular delivery of curcumin didecanoate prodrug: preparation, characterization and in vivo evaluation.

The objective of this study was to prepare the nanocrystals of curcumin didecanoate (CurDD) by wet ball milling and to investigate the comparative pharmacokinetics of oily nano- and micro-suspensions after intramuscular (i.m.) administration to rats.

Stabilization of zeylenone in rat plasma by the presence of esterase inhibitors and its LC-MS/MS assay for pharmacokinetic study.

Six esterase inhibitors, namely EDTA·2Na(+), NaF, phenylmethanesulfonyl fluoride, dichlorvos, bis-nitrophenyl phosphate (BNPP) and thenoyltrifluoroacetone, and the mixture of NaF and BNPP, were evaluated for the stabilization of labile benzoate containing zeylenone in rat plasma. The mixture appeared to exhibit the most effectively stabilizing effect with the degraded content of zeylenone decreasing from >60% (in the absence of inhibitors) to <6%. Following the stabilization by the addition of NaF (5 mM) and BNPP (5 mM), the analytes in rat plasma were acidified by formic acid and extracted into ethyl acetate at 0°C.

Disruption of HepG2 cell adhesion by gold nanoparticle and Paclitaxel disclosed by in situ QCM measurement.

Cell adhesion is a crucial issue for cytotoxicity or anticancer effectiveness for tumor cells. However, how both nanoparticles and drugs affect cell adhesion has not yet been defined. Herein, we report for the first time that gold nanoparticles and Paclitaxel can disrupt adhesion, as well as enhance apoptosis of HepG2 cell individually and synergistically, as observed by in situ measurement using quartz crystal microbalance (QCM).