SNCG shRNA suppressed breast cancer cell xenograft formation and growth in nude mice.

Background: Overexpression of breast cancer-specific gene 1 (SNCG) is associated with poor prognosis in advanced breast cancer patients. This study aimed to determine the effects of SNCG knockdown in breast cancer cells by using small hairpin RNA (shRNA).

Methods: Four different SNCG shRNA oligonucleotides were designed and chemically synthesized to construct mammalian expression vectors.