Impact of the Coronavirus disease pandemic on early breast cancer.

Objective: To assess the impact of the COVID-19 pandemic on the presentation, treatment, and survival of patients with early breast cancer (Stage I-III).

Methods: This study utilized data from the Surveillance, Epidemiology, and End Results database from January 1, 2018, to December 31, 2020. Patients diagnosed with primary breast cancer in 2020 were compared to those diagnosed in 2018 or 2019.

Electrosprayed Ultra-Thin Coating of Ethyl Cellulose on Drug Nanoparticles for Improved Sustained Release.

In nanopharmaceutics, polymeric coating is a popular strategy for modifying the drug release kinetics and, thus, new methods for implementing the nanocoating processes are highly desired. In the present study, a modified coaxial electrospraying process was developed to formulate an ultra-thin layer of ethyl cellulose (EC) on a medicated composite core consisting of tamoxifen citrate (TAM) and EC. A traditional single-fluid blending electrospraying and its monolithic EC-TAM nanoparticles (NPs) were exploited to compare.

[Pathogen Diagnosis of a Febrile HIV Case by the Metagenomic Next-generation Sequencing].

This study is aimed to explore the value of metagenomic next-generation sequencing (mNGS) in diagnosing pathogen in fever patients. It is often a challenge to identify the pathogen that caused the infection in the HIV patients with fever. How could the mNGS be helpful for pathogen diagnosis is unclear.

Rapid and visual detection for hypochlorite of an AIE enhanced fluorescence probe.

In this paper, a new 'turn-on' fluorescence probe for the rapid, sensitive, and visual detection of hypochlorite is reported. The push-pull type trianiline-tricyanofuran-based fluorescent probe was prepared using a condensation reaction between tricyanofuran and the thiophene-trianiline derivative that had high quantum yields and showed aggregation-induced emission enhanced properties. Upon exposure to hypochlorite, prominent fluorescence enhancement of the probe was observed via the release of the fluorophore from the probe.

Two-channel near-infrared fluorescence Ag ion sensing of a new star-shaped dendrimer.

A new near-infrared fluorescence sensor PDI-PD for Ag ions was successfully prepared and its structure characterized by H nuclear magnetic resonance (NMR), C NMR and high-resolution mass spectrometry; matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (HRMS MALDI-TOF). The probe exhibited rapid, sensitive, and selective two-channel fluorescence responses towards Ag ions and protons. The probe has a marked high binding affinity and high sensitivity for Ag , with a detection limit of 1.

The Process⁻Property⁻Performance Relationship of Medicated Nanoparticles Prepared by Modified Coaxial Electrospraying.

In pharmaceutical nanotechnology, the intentional manipulation of working processes to fabricate nanoproducts with suitable properties for achieving the desired functional performances is highly sought after. The following paper aims to detail how a modified coaxial electrospraying has been developed to create ibuprofen-loaded hydroxypropyl methylcellulose nanoparticles for improving the drug dissolution rate. During the working processes, a key parameter, i.

[Analysis of Pathogens and Clinical Characteristics in 157 Cases with Infective Endocarditis].

Objective: To analyze the clinical characteristics of infective endocarditis (IE) in culture-positive patients,so as to provide the evidences for reasonable diagnosis and treatment of IE.

Methods: We performed a retrospective study of 157 culture-positive IE cases,which were diagnosed according to modified Duke criteria for IE from Jan. 2008 to Aug.

Gracilibacillus eburneus sp.nov., a moderately halophilic bacterium isolated from Xinjiang province, China.

A novel Gram-staining positive, moderately halophilic, endospore-forming, motile, rod-shaped and strictly aerobic strain, designated YIM 93565, was isolated from a salt lake in Xinjiang province of China and subjected to a polyphasic taxonomic study. Strain YIM 93565 grew in the range of pH 6.0-9.

Skermanella rubra sp. nov., a bacterium isolated from the desert of Xinjiang, China.

A Gram-negative, pink-coloured, rod-shaped, motile bacterium, designated YIM 93097(T), was isolated from the desert soil collected from Xinjiang province of China. Strain YIM 93097(T) was found to grow at 20-45 °C (optimum 28-37 °C), pH 5.0-7.

[Analysis of resistance tendency of bloodstream-infecting pathogens in China].

Objective: To investigate the resistance profiles and the trend of bloodstream-infecting pathogens isolated from hospitalized patients during 2004-2010.

Methods: The bloodstream isolates were collected from 18 hospitals in 17 cities. Minimum inhibition concentrations (MIC) were determined using the agar dilution method recommended by CLSI (Clinical and Laboratory Standards Institute), and susceptibility results were analyzed according to the 2011 CLSI guideline.

[Clonal relatedness of bla(OXA-58)--carrying Acinetobacter baumannii clinical isolates].

Objective: To investigate the clonal relatedness of local bla(OXA-58)-carrying Acinetobacter baumannii clinical isolates.

Methods: Non-duplicated isolates of Acinetobacter baumannii were collected in West China Hospital and verified by recA sequencing. Acquired bla(OXA-58) gene and natural bla(OXA51/66) genes were detected by PCR.

[Effects of bone morphogenetic protein-7 therapy on E3 ubiquitin ligase expression in mouse liver with experimentally induced fibrosis].

Objective: This study explored the dynamic expression of the E3 ubiquitin-protein ligase gene, Arkadia, in response to carbon tetrachloride (CCl4)-induced liver fibrosis in a mouse model and investigated the differential expression that occurs following treatment with the anti-fibrotic bone morphogenetic protein-7 (BMP-7).

Methods: Thirty healthy male imprinting control region (ICR) mice were randomly assigned to three groups: normal (control; n = 6), CCl4-induced model group (model; n = 18), and CCl4-induced model with BMP-7 treatment group (treatment; n = 6). The model group was further divided into three subgroups (n = 6 each) for analysis at 4, 8 and 12 weeks after fibrosis induction.

Bone morphogenetic protein 7 suppresses the progression of hepatic fibrosis and regulates the expression of gremlin and transforming growth factor β1.

Bone morphogenetic protein 7 (BMP-7), a member of the transforming growth factor (TGF)-β superfamily, counteracts the effect of TGF-β through different signaling pathways, and gremlin is considered as one of the antagonists of BMP-7. The aim of this study was to investigate the antifibrotic effect of BMP-7, and to clarify the expression patterns of gremlin and TGF-β1 in the progression of hepatic fibrosis after treatment with BMP-7. A mouse liver fibrosis model was induced by hypodermic injection of CCL4 and all the liver and blood samples were preserved for further study.

[Dynamic expression of tropomyosin 1 in rat model of hepatic fibrosis and hepatic stellate cells].

Objective: To investigate the dynamic expression of TPM1 in rat model of hepatic fibrosis and hepatic stellate cells induced by TGFβ1.

Method: Thirty male SD rats were divided into control group (n = 6) and model group (n = 24). The rat model of hepatic fibrosis was established by intraperitoneal injection of dimethylnitrosamine(DMN).

[Diagnosis of infective endocarditis by Duke criteria and a new national criteria in a cohort of 205 patients].

Objective: To compare the value of the new national criteria (2 major or one major plus 3 minor criteria) with the Duke criteria for diagnosis of infective endocarditis (IE).

Methods: A total of 205 patients with clinical diagnosis of IE admitted at West China Hospital of Sichuan University were included in this study. Among them, IE was pathologically confirmed in 97 patients.

Clinical evaluation of oral levofloxacin 500 mg once-daily dosage for treatment of lower respiratory tract infections and urinary tract infections: a prospective multicenter study in China.

Levofloxacin (LVFX), a fluoroquinolone agent, has a broad spectrum that covers Gram-positive and -negative bacteria and atypical pathogens. It demonstrates good clinical efficacy in the treatment of various infections, including lower respiratory tract infections (LRTIs) and urinary tract infections (UTIs). To evaluate the efficacy and safety of oral LVFX 500 mg once daily, a large open-label clinical trial was conducted in 1266 patients (899 with LRTIs and 367 with UTIs) at 32 centers in China.

[Study on the correlation between resistance phenotype and expression level of femA of Staphylococcus aureus].

Objective: To detect the expression level of femA of Staphylococcus aureus strains with different phenotype.

Methods: 15 strains of the non-beta-lactamase-producing clinical isolates with different phenotype by agar dilution and by nitrocephin paper strip method were chosen as the object of test, in addition to 4 donative strains (BB270, BB308, BB586, COL). Total RNA were extracted and analysed by agarose gel electrophoresis.

[A study on the mechanism of drug resistance in Staphylococcus aureus].

Objective: To inquire into the mechanism of drug resistance in Staphylococcus aureus.

Methods: A total of 198 strains of Staphylococcus aureus were isolated from the samples sent to the Clinical Laboratory of Microbiology,West China Hospital. The resistance of Staphylococcus aureus to Methicillin was assayed with agar dilution.

[Investigating the fluoroquinolone molecular resistant mechanism of Stenotrophomonas maltophilia].

Objective: To research DNA gyrase and topoisomerase IV quinolone resistance-determining regions (QRDRs) in Stenotrophomonas maltophilia clinical isolates with different resistant levels of quinolone susceptibility.

Methods: We selected five strains for which the MIC of ciprofloxacin were higher than 2 mg/L and were negative for efflux mechanism; then we amplified their QRDRs of gyrA and parE, purified the fragment, and analyzed the nucleotide sequences.

Results: In Stenotrophomonas maltophilia DNA gyrA, the changes at positions 83 and 87 commonly involved in quinolones resistance in gram-negative bacteria were absent, and there was Gln but not Ser or Thr.

[The adverse reactions of parenteral norvancomycin in 1031 patients].

Objective: To investigate the safety of norvancomycin, and provide basis for its rational use in clinical practice.

Methods: We documented all adverse events occurred in inpatients who receive intravenous infusion of norvancomycin, then we evaluated the relationship between adverse events and norvancomycin and calculated the rates of adverse reaction.

Results: 1031 patients were enrolled in this study from March 2002 to June 2003 and 965 of them could be evaluated.

[Surveillance on antibiotic resistance of Stenotrophomonas maltophilia in Chengdu and Chongqing area].

Objective: To survey the antibiotic resistance of Stenotrophomonas maltophilia in Chengdu and Chongqing area and guide the rational antibiotics usage in the treatment of Stenotrophomonas maltophilia infection.

Methods: Minimal inhibitory concentrations (MICs) of 9 antibiotics against Stenotrophomonas maltophilia were measured using two-fold agar dilution method.

Results: A total of 154 strains of Stenotrophomonas maltophilia are multi-drug resistant.