[Inhibitory Effect of Metformin and Arsenic Trioxide on KG1a Cell Proliferation].

Objective: To investigate the effect of metformin and arsenic trioxide on KG1a cells proliferation of acute myeloid leukemia and its possible mechanism.

Methods: CCK-8 method was used to detect the killing effect of metformin, arsenic trioxide and combined application on KG1a cells. Annexin V-FITC/PI Dual Stain Flow Cytometry was used to detect the effect of combined application on apoptosis of KG1a cells.

Effects of Spermidine on Mouse Gut Morphology, Metabolites, and Microbial Diversity.

Spermidine is a class of biologically active organic small molecules that play an important role in maintaining intestinal homeostasis. The specific objective of this study was to explore the effects of spermidine on intestinal morphology, metabolites, and microbial diversity in mice. We showed that 0.

[Effect of DR4 Demethylation to the Proliferation and Apoptosis of Myeloid Leukemia K562 Cells].

Objective: To investigate the effect of tumor necrosis factor death receptor (DR) 4 demethylation to the proliferation and apoptosis of myeloid leukemia K562 cells.

Methods: The logarithmic phase of K562 cells were treated by desitabine (DCA) at 0, 0.8, 1.

[Anti-mouse CD122 antibody promotes the hematopoietic repopulating capacity of cord blood CD34⁺ cells in NOD/SCID mice].

The study was aimed to investigate the effect of anti-mouse CD122 antibody on the hematopoietic repopulating capacity of cord blood CD34⁺ cells in a humanized murine model-non obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. After sublethal irradiation with γ-ray, NOD/SCID mice were intraperitoneally injected with 200 µg mouse isotype control antibody or anti-mouse CD122 antibody. Human cord blood CD34⁺ cells or phosphate-buffered saline (PBS) were injected via the tail vein at 6-8 hours later.

The diagnostic and prognostic value of serum YKL-40 in endometrial cancer.

Purpose: To evaluate the diagnostic and prognostic value of serum YKL-40 in endometrial cancer (EC).

Methods: Serum YKL-40 levels were detected and compared in 34 of the 50 cases with EC before surgery, in 22 of the 34 with EC after surgery, in 30 cases with uterine myoma, and in 30 healthy women as normal controls. Receiver operating characteristics (ROC) curves were adopted for diagnosis and calculation of area under each ROC curve in EC.

Expression of HLA-I, CD8, and CD4 and Their Clinical Significance in Cervical Cancer.

Background: The local tissue immune status may play a role in the progression of cervical cancer. The aim of our study is to examine the expression of HLA-I, CD8 and CD4 in various cervical diseases and investigate their association with cervical cancer.

Methods: We chose the tissues of cervical cancer, cervical intraepithelial neoplasia (CIN), chronic cervicitis and peri-cancer tissues, and then detected the expression of HLA-I, CD8 and CD4 using SP immunohistochemistry.

[Effects of transforming growth factor and recombinant human bone morphogenetic protein 2 on the proliferation and differentiation of human periodontal ligament fibroblasts].

Objective: To investigate the effects of Transforming growth factor beta (TGFbeta) and recombinant human bone morphogenetic protein 2 (rhBMP2) on the proliferation and differentiation of human periodontal ligament fibroblasts (HPDLFs).

Methods: To observe the individual, simultaneous and sequential effects of TGFbeta and rhBMP2 on the proliferation, alkaline phosphatase (ALP) activity, osteocalcin (OC) synthesis and mineralized nodule formation of the cultured HPDLFs.

Results: TGFbeta significantly stimulated the proliferation of HPDLFs but had no effects on ALP activity, OC synthesis and mineralized nodule formation.