The effect of previous eradication failure on antibiotic resistance of Helicobacter pylori: A retrospective study over 8 years in Beijing.

Background: Antibiotic resistance is the main cause of Helicobacter pylori (H. pylori) treatment failure. This study aimed to explore the characteristics of antibiotic resistance of H.

A novel electrochemical biosensor for exosomal microRNA-181 detection based on a catalytic hairpin assembly circuit.

Exosomal microRNAs (miRNAs) derived from different cells are proposed to be important noninvasive biomarkers for the diagnosis of cardiovascular disease. Recently, sensitive and reliable sensing of exosomal miRNAs has been garnered significant attention. Herein, a novel electrochemical biosensor based on a step polymerization catalytic hairpin assembly (SP-CHA) circuit is designed for exosomal miR-181 detection.

Disordered intestinal microbes are associated with the activity of Systemic Lupus Erythematosus.

Intestinal dysbiosis is implicated in Systemic Lupus Erythematosus (SLE). However, the evidence of gut microbiome changes in SLE is limited, and the association of changed gut microbiome with the activity of SLE, as well as its functional relevance with SLE still remains unknown. Here, we sequenced 16S rRNA amplicon on fecal samples from 40 SLE patients (19 active patients, 21 remissive patients), 20 disease controls (Rheumatoid Arthritis (RA) patients), and 22 healthy controls (HCs), and investigated the association of functional categories with taxonomic composition by Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt).

[Identification of Trypanosoma Species in Rattus flavipectus and R. losea using Microscopy and PCR Method].

Objective: To identify two suspected Trypanosoma species in Rattus found in Zhejiang Province using microscopy and PCR method.

Methods: Blood samples were collected from Rattus losea and R. flavipectus.

[Epidemiological Analysis of Malaria in Wenzhou City during 2007-2014].

A total of 258 malaria cases with 2 deaths were reported during 2007-2014, including 148 vivax malaria cases, 106 falciparum malaria cases, and 4 ovale malaria cases. During 2007-2009, 86.0% (135/157) were vivax malaria cases with 3 indigenous cases.

[Epidemiological analysis of malaria in Wenzhou City in 2013].

Objective: To understand the characteristics of malaria prevalence in Wenzhou City in 2013, so as to provide the evidence for improving the elimination program of malaria.

Methods: The epidemiological data of malaria in Wenzhou City in 2013 were collected from the Chinese information system for disease control and prevention, and analyzed with the descriptive epidemiological methods for epidemiological characteristics of malaria.

Results: Totally 34 imported malaria cases were reported in Wenzhou City in 2013 with the incidence of 0.

Isolation and characterization of a novel arenavirus harbored by Rodents and Shrews in Zhejiang province, China.

To determine the biodiversity of arenaviruses in China, we captured and screened rodents and shrews in Wenzhou city, Zhejiang province, a locality where hemorrhagic fever diseases are endemic in humans. Accordingly, arenaviruses were detected in 42 of 351 rodents from eight species, and in 12 of 272 Asian house shrews (Suncus murinus), by RT-PCR targeting the L segment. From these, a single arenavirus was successfully isolated in cell culture.

[Diagnosis and analysis of the first imported ovale malaria case in Wenzhou City].

The first imported case of Plasmodium ovale infection in Wenzhou City was confirmed by microscopy and PCR test. The patient returned from the People's Republic of Congo to Wenzhou for three and a half months presented a history of fever with chills and rigors on April 30, 2012. The results from peripheral blood smear examination and PCR analysis confirmed a diagnosis of P.

Cross-species transmission in the speciation of the currently known murinae-associated hantaviruses.

To gain more insight into the phylogeny of Dabieshan virus (DBSV), carried by Niviventer confucianus and other Murinae-associated hantaviruses, genome sequences of novel variants of DBSV were recovered from Niviventer rats trapped in the mountainous areas of Wenzhou, China. Genetic analyses show that all known genetic variants of DBSV, including the ones identified in this study, are distinct from other Murinae-associated hantaviruses. DBSV variants show geographic clustering and high intraspecies diversity.

A new subtype of Thottapalayam virus carried by the Asian house shrew (Suncus murinus) in China.

Recently, many novel hantavirus species carried by insectivore had been found. In order to know whether the insectivore-associated hantaviruses exist in China, Asian house shrew (Suncus murinus) were analyzed for the presence of hantaviruses. 362 Asian house shrews were captured in Wenzhou of Zhejiang province.

[Correlation analysis on normalized difference vegetation index (NDVI) of different vegetations and climatic factors in Southwest China].

Based on the 1982-2006 NDVI remote sensing data and meteorological data of Southwest China, and by using GIS technology, this paper interpolated and extracted the mean annual temperature, annual precipitation, and drought index in the region, and analyzed the correlations of the annual variation of NDVI in different vegetation types (marsh, shrub, bush, grassland, meadow, coniferous forest, broad-leaved forest, alpine vegetation, and cultural vegetation) with corresponding climatic factors. In 1982-2006, the NDVI, mean annual temperature, and annual precipitation had an overall increasing trend, and the drought index decreased. Particularly, the upward trend of mean annual temperature was statistically significant.

Preparation of seeding type immobilized microorganisms and their degradation characteristics on Di-n-butyl phthalate.

Objective: To study the preparation of seeding type immobilized microorganisms and their degradation characteristics on di-n-butyl phthalate (DBP).

Methods: Diatomite, clinoptilolite, silk zeolite, and coal fly ash were chosen as reserved materials and modified. Their adsorption capacity and intensity in the bacteria were determined and the best carrier was picked out.

[A correlative study on heterotrophic bacteria and the main pollutant in city lakes' water].

Objective: To provide scientific basis for bioremediation of city lake, the distribution of heterotrophic bacteria and its correlation with major pollutions condition were studied.

Methods: Puping Lake and Moshui Lake of Wu Han City were choosen as the objects of our study. COD(cr) TOC, TP and TN were determined in sampled freshwater and sediment via the standard methods.