Effects of different anesthesia methods on labor process and postpartum serum estrogen and progesterone levels in primiparas with painless labor.

Objective: To look into the effects of different anesthesia methods on the labor process and the expression of serum estrogen and progesterone in primiparas with painless labor.

Methods: 60 primiparas receiving painless labor were selected as the research objects, and they were divided into either a Spinal & Continuous epidural anesthesia group (n = 30) or a continuous epidural anesthesia group (n = 30), anesthesia is administered using the corresponding anesthesia method. The authors compared serum estrogen and progesterone, inflammatory index expression, pain degree and neonatal health status in different periods.

mAexpress-enet: Predicting the regulatory expression mA sites by an enet-regularization negative binomial regression model.

As the most abundant mRNA modification, mA controls and influences many aspects of mRNA metabolism including the mRNA stability and degradation. However, the role of specific mA sites in regulating gene expression still remains unclear. In additional, the multicollinearity problem caused by the correlation of methylation level of multiple mA sites in each gene could influence the prediction performance.

Formulation of a Self-Nanoemulsifying Drug Delivery System of Buckwheat Flavonoids and Evaluation of Its Antimicrobial Activity.

This study was aimed at constructing a self-nanoemulsifying drug delivery system of buckwheat flavonoids and evaluating its antimicrobial activity. The construction of the nanoemulsion followed a pseudo-ternary phase diagram, and its particle properties (particle size, zeta potential, and surface morphology) and physicochemical parameters (turbidity, surface tension, pH value, conductivity, encapsulation efficiency, and stability) were evaluated. The antimicrobial potential of buckwheat flavonoids nanoemulsion was determined against Staphylococcus aureus, Escherichia coli, and Candida albicans and compared to the buckwheat flavonoids suspension.

[Effects of endothelial progenitor cells on proliferation and biological function of hepatic stellate cells under shear stress].

Objective: To investigate the effects of endothelial progenitor cells (EPCs) under shear stress on the biological function such as proliferation, adhesion, migration, apoptosis and expression of α-smooth muscle actin (α-SMA), collagen-I and collagen-Ⅲ of hepatic stellate cells (HSCs).

Methods: HSCs and EPCs were inoculated into the upper and lower layers of the co-culture chamber respectively and co-incubated for 24 hours. Then, 12 dyne/cm shear stress was applied to EPCs cells for another 24 hours.

Function of Krüppel‑like factor 2 in the shear stress‑induced cell differentiation of endothelial progenitor cells to endothelial cells.

The present study aimed to evaluate the effects of Krüppel‑like factor 2 (KLF2) on the differentiation of endothelial progenitor cells (EPCs) to endothelial cells (ECs) induced by shear stress, and to investigate the corresponding mechanisms. Cultured rat late EPCs were exposed to shear stress (12 dyn/cm2) for different lengths of time. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to measure the initial KLF2 mRNA levels in each group.

[Effects of inward rectifier potassium channel blockers on EPCs function].

Objective: To investigate the effects of inward rectifier potassium channel blockers (BaCl2, CsCl) on the functions of endothelial progenitor cells (EPCs).

Methods: Density gradient centrifugation-isolated rat hone marrow mononuclear cells were cultured in vitro. EPCs were harvested and seeded on six culture dish when cells grew to 3-5 passages.

A scheme for a single molecule phase-shift gate in a solid matrix.

We propose a feasible scheme to implement a phase-shift gate ((1 0) (0 eiγ)) based on a two-state single molecule in a solid matrix, where γ is a geometric phase controlled through a fast on-resonant laser field and a slow off-resonant radio-frequency field. In our scheme, a non-Hermitian quantum model is employed to characterize the single molecule in a solid matrix including the spontaneous decay effect. By the coupling between the radio-frequency field and the two-state permanent dipole difference resulting from the solid matrix, the spontaneous decay fatal to the preservation of geometric phase can be effectively suppressed for a considerably long waiting time.

Protective effects of the active fraction from the tuber of Scirpus yagara in mouse endotoxin shock model.

Ethnopharmacological Relevance: Scirpus yagara Ohwi is a perennial, aquatic plant, whose dry tubers have long been used as Traditional Chinese Medicine (TCM) "Sanleng" for the treatment of postpartum abdominal pain, hyperemesis gravidarum, amenorrhea, dyspepsia and several inflammatory related diseases. Although it is known to have anti-inflammatory activities, its mechanism of action on lipopolysaccharide (LPS)-induced inflammation has not yet been identified in detail.This study was designed to investigate the anti-inflammatory activity of the active fraction (AF) from the tuber of Scirpusyagara both in vitro and in vivo.

Unification of the family of Garrison-Wright's phases.

Inspired by Garrison and Wight's seminal work on complex-valued geometric phases, we generalize the concept of Pancharatnam's "in-phase" in interferometry and further develop a theoretical framework for unification of the abelian geometric phases for a biorthogonal quantum system modeled by a parameterized or time-dependent nonhermitian hamiltonian with a finite and nondegenerate instantaneous spectrum, that is, the family of Garrison-Wright's phases, which will no longer be confined in the adiabatic and nonadiabatic cyclic cases. Besides, we employ a typical example, Bethe-Lamb model, to illustrate how to apply our theory to obtain an explicit result for the Garrison-Wright's noncyclic geometric phase, and also to present its potential applications in quantum computation and information.

[Effects of extracellular matrix on biological characteristics of late endothelial progenitor cells].

The present study was designed to investigate the effects of various extracellular matrix (ECM) proteins on the biological characteristics of late endothelial progenitor cells (EPCs). Density gradient centrifugation-isolated rat bone marrow mononuclear cells were cultured in complete M199 medium, which contained 15% fetal calf serum, 10 μg/L vascular endothelial growth factor (VEGF) and 5 μg/L basic fibroblast growth factor (bFGF). EPCs were plated on substrates containing fibronectin (Fn), laminin (Ln) or rat tail tendon collagen (Col), and the corresponding cells were defined as Fn, Ln and Col groups.

[Anticancer activity of Crocodylus siamensis blood in vitro and in vivo].

Objective: To study the anticancer effects of the blood of Crocodylus siamensis in vitro and in vivo.

Methods: The inhibitory effects of serum and plasma of Crocodylus siamensis on proliferation of HepG2, BGC823, HeLa and SKOV3 cell were measured by MTT assay. The mouse S180 tumor model was used to evaluate the anti-tumor effect in vivo.

Preparation and properties of rutin-hydrolyzing enzyme from tartary buckwheat seeds.

A rutin hydrolyzing enzyme (RHE) was isolated from Fagopyrum tataricum Moench seeds by using ammonium sulphate fractionation, anion exchange and size exclusion chromatography. The purified RHE has an apparent molecular weight of about 70kDa determined by SDS-PAGE, with an isoelectric point (pI) (determined by isoelectric focusing) of 6.7.

[Preparation and identification of the rat antibody against recombinant buckwheat trypsin inhibitor].

Aim: To prepare the rat antibody against the recombinant buckwheat trypsin inhibitor.

Methods: The recombinant buckwheat trypsin inhibitor was expressed in E.coli.

Molecular cloning, recombinant expression, and immunological characterization of a novel allergen from tartary buckwheat.

Buckwheat is generally regarded as a nutritionally rich food source. However, earlier studies prove that it also causes allergies to subjects. Allergenic proteins with a strong IgE-binding activity have been identified in common buckwheat (CB) and a 24 kDa allergen (rTBa) in tartary buckwheat (TB).

[Effects of centipede extract on life span of drosophila].

Objective: To evaluate effects of centipede on life span of drosophila and on the appearance of the filial generation.

Methods: Drosophila melanogaster (male and female) were cultured in tubes with centipede extraction at concentrations of 0, 0.2%, 1%, 2.