Integration of single-nuclei transcriptome and bulk RNA-seq to unravel the role of AhWRKY70 in regulating stem cell development in Arachis hypogaea L.

Peanut stem is a vital organ to provide mechanical support and energy for aerial tissue development. However, the transcriptional regulatory mechanisms underlying stem development at a single-cell resolution remain unclear. Herein, single-nuclei isolation coupled with fluorescent-activated cell sorting was employed to construct a cell atlas of peanut seedling stems using microdroplets-based single-nuclei RNA-sequencing.

The Management Modality of Aseptic Loosening for Acetabular Components Along With Femur Prostheses Following Primary Hip Arthroplasty: A Reports of Four Cases.

Purpose: Aseptic loosening (AL) of prostheses is a complex and multi-factorial consequences characterized by nonspecific hip start-up pain, impaired gait, or ambulation. The AL of acetabular components associated with femur prostheses can lead to challenges in accurate diagnosis and suitable therapy, potentially resulting in disaster consequence. This study reported revision of in four cases with AL of acetabular components associated with or without femur prostheses after underwent primary total hip arthroplasty.

The membrane may be a key factor influencing browning: a mini review on browning mechanisms of fresh-cut fruit and vegetables from a multi-omics perspective.

Fresh-cut fruit and vegetables are susceptible to browning during storage and subsequent consumption. The cell membrane acts as a vital structural barrier, compartmentalizing various substances within living organisms. The fresh-cutting process induces mechanical injuries, disrupting these membranes and resulting in the leakage of cellular contents.

Mechanical compression induces chondrocyte hypertrophy by regulating Runx2 O-GlcNAcylation during temporomandibular joint condyle degeneration.

Aims: Excessive chondrocyte hypertrophy is a common feature in cartilage degeneration which is susceptible to joint overloading, but the relationship between mechanical overloading and chondrocyte hypertrophy still remains elusive. The aim of our study was to explore the mechanism of mechanical compression-induced chondrocyte hypertrophy.

Methods: In this study, the temporomandibular joint (TMJ) degeneration model was built through forced mandibular retrusion (FMR)-induced compression in TMJ.

Morphological alterations of the thymus gland in individuals with schizophrenia.

Despite its critical function in the immune system and accumulating evidence of immunological abnormalities in schizophrenia, the thymus has long been overlooked. We aimed to investigate thymic morphological alterations and their corresponding heterogeneity in patients with schizophrenia. Imaging-derived thymic morphology was assessed and compared between 419 patients with schizophrenia and 460 age- and sex-matched control participants aged 16-40 years who underwent chest computed tomography (CT) scanning.

Specific cognitive impairment predicts the neuropsychiatric symptoms in patient with mild cognitive impairment.

Background: Neuropsychiatric symptoms (NPS) are common in mild cognitive impairment (MCI). However, knowledge is limited about the relationship of NPS, clinical factors, and cognition in MCI.

Methods: A total of 1099 dementia, 1323 MCI and 377 cognitively normal (CN) were selected from the Tongji Cohort Study of Aging.

Comparison of key floral components between two Tilia species and among different processing technologies as revealed by widely targeted metabolomic profiling.

Background: Tilia has a long history of cultivation and holds high ornamental and economic value. The volatile aroma compounds of Tilia flowers have significant characteristics that contribute to their ornamental appeal, and affect the flavor of floral tea. Here, widely targeted metabolomic analyses were conducted to investigate the aroma active compounds in the fresh inflorescences of Tilia cordata Mill.

[Optimization of centrifugal artificial heart pump blade parameters based on back propagation neural network and grey wolf optimization algorithm].

The impeller, as a key component of artificial heart pumps, experiences high shear stress due to its rapid rotation, which may lead to hemolysis. To enhance the hemolytic performance of artificial heart pumps and identify the optimal combination of blade parameters, an optimization design for existing pump blades is conducted. The number of blades, outlet angle, and blade thickness were selected as design variables, with the maximum shear stress within the pump serving as the optimization objective.

Experimental investigation on flexural fatigue performance of recycled aggregate concrete hybrid with basalt-polyacrylonitrile fiber.

To reduce the construction industry's reliance on raw natural resources and alleviate the environmental pressures caused by waste concrete, recycling waste concrete materials as recycled coarse aggregate in the production of new concrete presents a viable solution. This study aims to expand the application of recycled aggregate concrete (RAC) in road and bridge engineering. In this experiment, twelve concrete mixtures were designed to investigate the effects of basalt fiber (BF) and polyacrylonitrile fiber (PANF) on the performance of RAC.

[Application of colloidal gold method and chemiluminescence method for detecting gonadotropins in morning urine to assess pubertal development status in children].

Objectives: To explore the application of the colloidal gold method and chemiluminescence method in detecting gonadotropin (Gn) in morning urine for assessing pubertal development status in children.

Methods: A total of 132 children diagnosed with central precocious puberty (CPP), early and fast puberty (EFP), and premature thelarche (PT) at Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine from November 2021 to December 2022 were included, along with 685 healthy children who underwent routine health examinations at the hospital's pediatric health care department during the same period. All 132 patients underwent a gonadotropin-releasing hormone (GnRH) stimulation test.

The effectiveness of dyadic interventions for health outcomes of prostate cancer patients and informal caregivers: a systematic review and meta-analysis.

Background: Prostate cancer is a worldwide health issue, and current prostate cancer care extends to the patient‒caregiver dyadic setting, where individuals are interdependent and interact with each other as well as possible negative psychological and behavioural outcomes. However, the impact of dyadic care interventions on health outcome indicators for prostate cancer patients and their informal caregivers has yet to be examined.

Aim: To describe the characteristics of dyadic interventions involving patients with prostate cancer and their informal caregivers and investigate their effects on psychosocial health, sexual health, and dyadic relationships.

Gastrointestinal complications of hepatic glycogen storage disease: a national survey questionnaire study in China.

Background: Hepatic glycogen storage diseases (GSD) are inborn errors of metabolism with abnormal storage or utilization of glycogen, a complex disease with significant genetic heterogeneity and similar clinical manifestations. This study aimed to describe the gastrointestinal symptoms and endoscopic features of hepatic GSD, including types Ia, Ib, III, VI, and IX, to provide evidence for etiology and treatment.

Methods: A national cohort survey questionnaire was distributed to patients diagnosed with GSD type Ia, Ib, III, VI, and IX through genetic testing or their parents in mainland China in May 2022.

Effects of dietary Lactococcus lactis Z-2 on growth, host health and resistance to Aeromonas hydrophila in juvenile common carp (Cyprinus carpio L.).

Background: Lactococcus lactis Z-2 was previously isolated from common carp intestine. In order to investigate the effects of optimal dose of L. lactis Z-2 on growth, host health and disease resistance to Aeromonas hydrophila in common carp, five experimental diets, including without (CK and CK+ groups) or with 5 × 10 (group A), 5 × 10 (group B) and 5 × 10 CFU g (group C) L.

Neurobiological fingerprints of negative symptoms in schizophrenia identified by connectome-based modeling.

Aim: As a central component of schizophrenia psychopathology, negative symptoms result in detrimental effects on long-term functional prognosis. However, the neurobiological mechanism underlying negative symptoms remains poorly understood, which limits the development of novel treatment interventions. This study aimed to identify the specific neural fingerprints of negative symptoms in schizophrenia.

Femoral neck fracture in a senile patient with primary hyperparathyroidism: A case report.

Rationale: As a rare cause of femoral neck fracture, usually, hyperparathyroidism is missed diagnosed by orthopedist. Patient can present with various disappearance of clinical manifestations. Primary hyperparathyroidism in senile male population is commonly an asymptomatic disorder discovered incidentally through routine lab testing.

[Clinicopathological Analysis of 14 Cases of Primary Pulmonary Lymphoepithelial Carcinoma].

Background: Primary pulmonary lymphoepithelial carcinoma (PPLEC) is a rare form of lung malignancy, accounting for only 0.7% of all lung cancers. It is currently classified as a distinct subtype within squamous cell carcinomas.

Salidroside Improves Oocyte Competence of Reproductively Old Mice by Enhancing Mitophagy.

The decline of oocyte quality with advanced maternal age has a detrimental effect on female fertility. However, there is limited knowledge of therapeutic options and their mechanisms to improve oocyte quality in reproductively older women. In this study, we demonstrated that supplementation of salidroside improves the oocyte quality of reproductively old mice.

Chemical profile changes in Peanut seeds infected with aspergillus flavus via widely targeted metabolomics.

Peanut seeds are enriched with protein and fatty acids, making them susceptible to infection by Aspergillus flavus (A. flavus). The infected seeds are harmful to human health due to the aflatoxin contamination.

Electroacupuncture ameliorates inflammatory pain through CB2 receptor-dependent activation of the AMPK signaling pathway.

Background: Chronic inflammatory pain is a pervasive condition, and electroacupuncture (EA) is an effective treatment, but its mechanisms are not fully understood. AMP-activated protein kinase (AMPK), a key energy sensor, is involved in pain relief and EA's effects. EA may work by increasing endocannabinoids, upregulating CB2 receptors (CB2R), and stimulating β-endorphin (β-END).

HM13 is a predictive biomarker of metastasis and neutrophil infiltration in colorectal cancer.

Background: High levels of histocompatibility minor 13 (HM13) have been related to the progression of several cancers. Here, we investigated the function of HM13 in colorectal cancer (CRC).

Methods: HM13 expression, clinicopathology analysis, and its influence on survival were analyzed in multiple public databases (TCGA, TIMER2.

[Research progress on "homologous different effects" of Aurantii Fructus Immaturus and Aurantii Fructus].

Aurantii Fructus Immaturus and Aurantii Fructus are the dried young and immature fruits of Rutaceae, which mainly originate from the same part of lime. They are harvested from the same part with different maturity levels, which leads to different medicinal materials. It has been reported that Aurantii Fructus Immaturus and Aurantii Fructus contain flavonoids, alkaloids, volatile oils, coumarins and other components, which have a variety of pharmacological effects such as gastrointestinal disorder-alleviating, anti-anxiety, lipid metabolism-modulating, anti-inflammatory, antimicrobial, and antioxidant effects.