Genotypic and phenotypic characteristics of ADGRV1 mutations in four children and functional validation in a zebrafish model.

Mutations in ADGRV1 can cause seizures, but the mechanism remains unclear. The zebrafish model can be used to assess the functions of human ADGRV1 and its variant alleles during embryonic development. In this study, we summarized the phenotypic and genotypic characteristics of four children with ADGRV1 variation and based on this, we validated the ADGRV1 loss phenotype in an adgrv1-knockout zebrafish model.

A colorectal liver metastasis prediction model based on the combination of lipoprotein-associated phospholipase A2 and serum biomarker levels.

Objective: This study aims to assess the predictive value of serum lipoprotein-associated phospholipase A2 (Lp-PLA2) in colorectal liver metastasis (CRLM) patients.

Methods: A total of 507 participants were recruited for this study, comprising 162 healthy controls (HCs), 186 non-CRLM patients, and 159 CRLM patients. Serum Lp-PLA2 levels were measured across these three groups, and a CRLM prediction model was developed using machine learning (ML) algorithms in conjunction with traditional serological markers.

[Clinical characteristics and long-term follow-up study of basal ganglia infarction after minor head trauma in infants and young children].

Objectives: To investigate the clinical characteristics and prognosis of infants and young children with basal ganglia infarction after minor head trauma (BGIMHT).

Methods: A retrospective analysis was conducted on the clinical data and follow-up results of children aged 28 days to 3 years with BGIMHT who were hospitalized at Children's Hospital of Soochow University from January 2011 to January 2022.

Results: A total of 45 cases of BGIMHT were included, with the most common symptom being limb movement disorders (96%, 43/45), followed by facioplegia (56%, 25/45).

Differential impacts of water diversion and environmental factors on bacterial, archaeal, and fungal communities in the eastern route of the South-to-North water diversion project.

Water diversion projects effectively mitigate the uneven distribution of water resources but can also influence aquatic biodiversity and ecosystem functions. Despite their importance, the impacts of such projects on multi-domain microbial community dynamics and the underlying mechanisms remain poorly understood. Utilizing high-throughput sequencing, we investigated bacterial, archaeal, and fungal community dynamics along the eastern route of the South-to-North water diversion project during both non-water diversion period (NWDP) and water diversion period (WDP).

Effect of nirmatrelvir/ritonavir (Paxlovid) on hospitalization among adults with COVID-19: An electronic health record-based target trial emulation from N3C.

Background: Nirmatrelvir with ritonavir (Paxlovid) is indicated for patients with Coronavirus Disease 2019 (COVID-19) who are at risk for progression to severe disease due to the presence of one or more risk factors. Millions of treatment courses have been prescribed in the United States alone. Paxlovid was highly effective at preventing hospitalization and death in clinical trials.

Characterization of the genomic landscape in liver oligometastatic NSCLC.

Objectives: Emerging data have shown that local treatment could provide clinical benefit for non-small cell lung cancer (NSCLC) patients with oligometastasis. Liver metastases have the worst prognosis in advanced NSCLC, but the genomic characteristics of liver oligometastasis remain unclear. The aim of our study was to elucidate the molecular features of liver oligometastatic NSCLC.

Ligation of the intersphincteric fistula tract (LIFT) for high transsphincteric fistulas: a double-center retrospective study with long-term follow-up.

Purpose: This study aimed to evaluate the long-term efficacy of the ligation of the intersphincteric fistula tract (LIFT) procedure in treating high transsphincteric fistulas.

Methods: We conducted a retrospective study to evaluate the success rate of LIFT treatment in 82 patients with high transsphincteric fistulas involving at least one-third of the external sphincter. This study was carried out across 2 centers from November 2009 to February 2023.

Validation of the Chinese version of the Epilepsy Anxiety Survey Instrument (EASI) and its brief version (brEASI) in Western China.

Objective: To translate and validate the Chinese version of the Epilepsy Anxiety Survey Instrument (EASI) and its brief version (brEASI) among Chinese people with epilepsy.

Methods: Adult outpatients from Sichuan Provincial People's Hospital were recruited. The type of anxiety disorder was determined via the Mini International Neuropsychiatric Interview (MINI).

Mapping the spatial atlas of the human bone tissue integrating spatial and single-cell transcriptomics.

Bone is a multifaceted tissue requiring orchestrated interplays of diverse cells within specialized microenvironments. Although significant progress has been made in understanding cellular and molecular mechanisms of component cells of bone, revealing their spatial organization and interactions in native bone tissue microenvironment is crucial for advancing precision medicine, as they govern fundamental signaling pathways and functional dependencies among various bone cells. In this study, we present the first integrative high-resolution map of human bone and bone marrow, using spatial and single-cell transcriptomics profiling from femoral tissue.

Revealing the Limitations of the Thermocapacitive Cycle.

While thermoelectric conversion by a thermocapacitive cycle has been considered a promising green technology for low-grade heat recovery, our study finds that its practical feasibility is overestimated. During thermal charging, the coexistence and dynamic competition between thermal-induced voltage rise and self-discharge lead to the limitations of the thermocapacitive cycle. Therefore, the operational conditions in the charge-heat-discharge steps seriously restrict the thermal charging performance.

NR1D1 Inhibition Enhances Autophagy and Mitophagy in Alzheimer's Disease Models.

Alzheimer's disease (AD) is marked by extracellular beta-amyloid (Aβ) plaques and intracellular Tau tangles, leading to progressive cognitive decline and neuronal dysfunction. Impaired autophagy, a process by which a cell breaks down and destroys damaged or abnormal proteins and other substances, contributes to AD progression. This study investigated Nuclear Receptor Subfamily 1 Group D Member 1 (NR1D1) as a potential therapeutic target for modulating autophagy.

Neutrophil Membrane Nanovesicles Alleviate the Renal Function Indicators in Acute Kidney Injury Caused by Septic Rats.

This study aims to explore the efficacy of neutrophil membrane nanovesicles (NMNVs) in the treatment of acute kidney injury caused by sepsis (S-AKI). Moreover, its effects on renal function indicators in plasma [creatinine (CREA), urea (UREA)], oxidative stress factor [malondialdehyde (MDA)], inflammatory factor [myeloperoxidase (MPO), histone H4 (H4), and macrophage inflammatory protein-2 (MIP-2)] are studied. Sixty SPF grade adult male Wistar rats in a healthy state under natural infection were randomly divided into blank, LSP, and experimental groups, with 20 rats in each group.

Efficient and Scalable Direct Regeneration of Spent Layered Cathode Materials via Advanced Oxidation.

Among direct recycling methods for spent lithium-ion batteries, solid-state regeneration is the route with minimal bottlenecks for industrial application and is highly compatible with the current industrial cathode materials production processes. However, surface structure degradation and interfacial impurities of spent cathodes significantly hinder Li replenishment during restoration. Herein, we propose a unique advanced oxidation strategy that leverages the inherent catalytic activity of spent layered cathode materials to address these challenges.

Twisted Cucurbit[14]uril-Based Supramolecular Self-Assembly Induces Fluorescence Emission of Dye Molecules for Multi-Channel Cell Imaging.

In this study, a supramolecular fluorescent material was constructed by using double-cavity twisted cucurbit[14]uril (tQ[14]) and positively charged Astrazon Pink FG (APFG) based on the non-covalent host-guest interaction for the first time. The thermodynamic parameters of the APFG@tQ[14] in aqueous solution were determined by isothermal titration calorimetry (ITC), the results indicated that the spontaneous assembly of APFG@tQ[14] is mainly driven by enthalpy. The intramolecular charge transfer (ICT) effect induced the APFG@tQ[14] probe to emit a strong orange-red fluorescence.

The comparison of uniportal versus multiportal video-assisted thoracic surgery for esophageal cancer: a propensity-weighted analysis.

Objectives: The utilization of uniportal video-assisted thoracoscopic surgery (VATS) has become prevalent, notwithstanding, there are few studies exploring its application specifically in esophagectomy.

Methods: A retrospective analysis was conducted on data collected from patients diagnosed with clinical stage T1-3/N0/M0 thoracic esophageal cancer, who underwent surgery between January 2017 and December 2020. To evaluate the outcomes, an analysis was conducted utilizing the inverse probability of treatment weighting (IPTW) method.

Synthesis of Mn-doped hollow octahedron ZnInS to enhance photocatalytic hydrogen production performance utilizing metal-organic framework as template.

Designing semiconductor photocatalysts with unique structures can improve the transfer efficiency of solar energy to hydrogen (H). In this study, a dual modification method of element doping and morphological control was used. The Mn-doped hollow octahedron ZnInS (ZHO-Mn) was synthesized by a simple one-pot solvothermal method using the octahedral Mn-based metal-organic framework (Mn-MOF) as a template.

Analysis of Immunometabolic Profiles in Patients With Chronic Drug-Induced Liver Injury and Validation in Mice to Reveal Potential Mechanisms.

Background: The mechanism underlying chronic drug-induced liver injury (DILI) remains unclear. Immune activation is a common feature of DILI progression and is closely associated with metabolism. We explored the immunometabolic profile of chronic DILI and the potential mechanism of chronic DILI progression.

Multi-Band Scattering Characteristics of Miniature Masson Pine Canopy Based on Microwave Anechoic Chamber Measurement.

Using microwave remote sensing to invert forest parameters requires clear canopy scattering characteristics, which can be intuitively investigated through scattering measurements. However, there are very few ground-based measurements on forest branches, needles, and canopies. In this study, a quantitative analysis of the canopy branches, needles, and ground contribution of Masson pine scenes in C-, X-, and Ku-bands was conducted based on a microwave anechoic chamber measurement platform.

Computer Vision-Based Fire-Ice Ion Algorithm for Rapid and Nondestructive Authentication of Ziziphi Spinosae Semen and Its Counterfeits.

The authentication of Ziziphi Spinosae Semen (ZSS), Ziziphi Mauritianae Semen (ZMS), and Hovenia Acerba Semen (HAS) has become challenging. The chromatic and textural properties of ZSS, ZMS, and HAS are analyzed in this study. Color features were extracted via RGB, CIELAB, and HSI spaces, whereas texture information was analyzed via the gray-level co-occurrence matrix (GLCM) and Law's texture feature analysis.

Prediction of Mechanical Properties and Fracture Behavior of TC17 Linear Friction Welded Joint Based on Finite Element Simulation.

TC17 titanium alloy is widely used in the aviation industry for dual-performance blades, and linear friction welding (LFW) is a key technology for its manufacturing and repair. However, accurate evaluation of the mechanical properties of TC17-LFW joints and research on their joint fracture behavior are still not clear. Therefore, this paper used the finite element numerical simulation method (FEM) to investigate the mechanical behavior of the TC17-LFW joint with a complex micro-structure during the tensile processing, and predicted its mechanical properties and fracture behavior.

The Effects of Nitrogen Alloying on the Microstructure and Properties of Cu-Bearing Antimicrobial Stainless Steel.

In this study, a novel Cu-bearing 304 stainless steel doped with 4.0 wt.% Cu (304-Cu SS) was developed, and the effects of nitrogen microalloying (304N-Cu SS) and heat treatment on mechanical, antibacterial, and corrosion properties were investigated.

Elucidating the Underlying Allelopathy Effects of on Using Metabolomics Profiling.

dominates the subalpine meadows in Shangri-La (Southwest China) owing to its potent allelopathic effects. However, the effects underlying its allelopathy require further characterization at the physiological and molecular levels. In this study, the physiological, biochemical, and metabolic mechanisms underlying allelopathy were investigated using as a receptor plant.