Publications by authors named "Xiao Qun Cao"

Reactive oxygen species (ROS) are pivotal signaling molecules that control a variety of physiological functions. As a member of the ROS family, peroxynitrite (ONOO) possesses strong oxidation and nitrification abilities. Abnormally elevated levels of ONOO can lead to cellular oxidative stress, which may cause several diseases.

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Cells of most eukaryotic species contain mitochondria, which play a role in physiological processes such as cellular senescence, metabolism, and autophagy. Viscosity is considered a key marker for many illnesses and is involved in several crucial physiological processes. Cyanide (CN) can target cytochrome-c oxidase, disrupting the mitochondrial electron transport chain and causing cell death through asphyxiation.

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Mitochondria are the powerhouses in cells, providing the energy needed for cellular activities. However, the abnormalities in the mitochondrial microenvironment (e.g.

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Ulcerative colitis, a kind of inflammatory bowel disease (IBD), is caused by dysregulated immune response of intestinal bacteria. This chronic disorder can lead to a deficiency of O (hypoxia) in the colon microenvironment. Nitroreductase (NTR) is a highly expressed endogenous enzyme under hypoxia, so the detection of NTR can provide diagnostic information about ulcerative colitis.

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Ulcerative colitis is a prevalent inflammatory disease caused by the intestinal bacterial infection. And it is related to the hypoxic degrees in the colon microenvironment. Hypoxia, a condition of imbalance in O supply and consumption, is accompanied by the overexpressed level of nitroreductase (NTR).

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Mitophagy, a mitochondria-selective autophagy process, plays critical roles in maintaining intracellular homeostasis by removing the damaged mitochondria and recycling the nutrients in a lysosome-dependent manner. Mitophagy process could result in the changes of mitochondrial pH. So fluorescent probes for detecting mitochondrial pH during mitophagy are highly needed for exploring the functions of mitochondria.

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Cyanide (CN) is a highly toxic compound that exists in many substances and is harmful to the environment and human health. Therefore, it is of great significance to develop excellent CN ion probes, especially solvent-induced on-off fluorescent probes. Based on the condensation reaction of indolo[2,1-b][1,3]oxazine molecules with aldehydes, probes (E)-13a-(2-(9-ethyl-9H-carbazol-3-yl)vinyl)-14,14-dimethyl-10-nitro-13a,14-dihydro-8H-benzo[e]benzo[5,6][1,3]oxazino[3,2-a]indole (NCO) and (E)-13a-(2-(9-benzyl-9H-carbazol-3-yl)vinyl)-14,14-dimethyl-10-nitro-13a,14-dihydro-8H-benzo[e]benzo[5,6][1,3]oxazino[3,2-a]indole (NBO) were synthesized to detect CN.

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As a member of the reactive oxygen species, hydrogen peroxide (HO) plays critical roles in oxidative stress and cell signaling. Intracellular abnormal levels of HO production are closely related to many diseases. Therefore, the real-time monitoring of HO in the cells is important.

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Hypochlorite (OCl) has long been recognized as an effective microbicidal agent in immune system. Herein, we report the design, preparation and spectral characteristics of a OCl fluorescent probe (FI-Mito). The probe exhibited remarkable fluorescence turn-on signal in the red region upon OCl titration with the detection limit as low as 0.

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Heat shock is a potentially fatal condition characterized by high body temperature (>40 °C), which may lead to physical discomfort and dysfunctions of organ systems. Acidic pH environment in lysosomes can activate enzymes, thus facilitating the degradation of proteins in cellular metabolism. Owing to the lack of a practical research tool, it remains difficult to exploit relationship between heat shock and lysosome.

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Article Synopsis
  • - The paper discusses a new molecular probe called CR-Ly that uses fluorescence resonance energy transfer to measure levels of lysosomal HOCl (hypochlorous acid) effectively.
  • - CR-Ly demonstrates high sensitivity, quick response, and excellent selectivity for HOCl, distinguishing it from other similar biological compounds.
  • - This probe allows researchers to image endogenous HOCl with low toxicity and is specifically designed to target lysosomes, making it a valuable tool for studying diseases related to HOCl in these organelles.
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In this paper, we synthesized a ratiometric fluorescence probe (IRh-Ly) for lysosomal hypochlorous acid (HOCl) by adopting a through-bond energy transfer (TBET) strategy on rhodamine-imidazo[1,5-a]pyridine platform. IRh-Ly showed brilliant selectivity, rapid response for HOCl. The probe also exhibited high sensitivity with the detection limit calculated to be 10.

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A rhodamine B-based derivative (RL1) was developed as a specific fluorescent probe for HOCl. Meanwhile, morpholine moiety was introduced into the probe. It was found that the probe could display highly selective, sensitive and naked-eye detection upon the addition of HOCl.

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A new ratiometric fluorescent probe based on Förster resonance energy transfer (FRET) for sensing lysosomal pH has been developed. The probe (RMPM) was composed of imidazo[1,5-α]pyridine quaternary ammonium salt fluorophore as the FRET donor and the rhodamine moiety as the FRET acceptor. It's the first time to report that imidazo[1,5-α]pyridine quaternary ammonium salt acts as the FRET donor.

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A series of novel 2-acyl-6-aryl substituted indolizine derivatives was synthesized by a novel tandem reaction between 4-acyl-pyrrole-2-carbaldehyde derivatives and ethyl 4-bromo-3-arylbut-2-enoate under mild conditions. The compounds were characterized using IR, (1)H NMR (13)C NMR and HRMS. The crystal structure of 7a was determined using single crystal X-ray crystallography.

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A series of novel imidazo[1,5-a]pyridine-hydrazone derivatives were synthesized and characterized by infrared spectroscopy (IR), 1H NMR, 13C NMR and high resolution mass spectrometer (HRMS). Typically, the spatial structure of compound 3j was determined using X-ray diffraction analysis. The UV-vis absorption and fluorescence spectral characteristics of the compounds in dichloromethane and acetonitrile were investigated.

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A series of novel 5-aryl-3-benzimidazolyl-1-phenyl-pyrazoline derivatives were synthesized by the reaction of benzimidazolyl chalcone and phenylhydrazine in 41-72% yields. The compounds were characterized using IR, (1)H NMR and HRMS. Absorption and fluorescence spectra were measured in different organic solvent.

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Novel imidazo[1,5-a]pyridinyl 1,3,4-Oxadiazole derivatives were synthesized and characterised by IR, (1)H NMR and HRMS.UV-vis absorption and fluorescence properties of these compounds in different solutions showed that the maximal emission wavelength was not significantly changed in different solvents; however, maximum absorption wavelength was blue-shifted with the increase of solvent polarity. Absorption λ(max) and emission λ(max) was less correlated with substituent groups on benzene rings.

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The title compound, C(21)H(18)N(2)O(2), was synthesized using a novel tandem annulation reaction between (1H-benzimidazol-2-yl)(phen-yl)methanone and (E)-ethyl 4-bromo-but-2-enoate under mild conditions. The dihedral angles between the mean planes of the five-membered imidazole ring and the pyridine, benzene and phenyl rings are 0.45 (6), 1.

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