Chemoenzymatic Synthesis of Core-Fucosylated Asymmetrical N-Glycans with Different-Length Oligo-N-Acetyllactosamine Motifs and Their Sialylated Extensions.

An efficient chemoenzymatic approach for the diversity-oriented synthesis of core-fucosylated asymmetrical N-glycans bearing different lengths of oligo-N-acetyllactosamine (LacNAc) and their sialylated extensions was described. Two oligosaccharide precursors were chemically synthesized by length-controlled introduction of oligo-LacNAc motifs through stereoselectively iterative glycosylation of a common hexasaccharide intermediate. Both oligosaccharide precursors can be well recognized by α1,6-fucosyltransferase FUT8 to generate core-fucosylated N-glycans, which was subjected to divergent enzymatic extension using a galactosyltransferase module and two sialyltransferase modules to provide a wide array of core-fucosylated asymmetrical biantennary N-glycans having different-length oligo-LacNAc motifs capped by various sialic acid-linkages.

Thrombus rooting in the pulmonary arteriovenous fistula in a patient with cryptogenic stroke, a case report.

Background: Cryptogenic stroke constitutes approximately 40% of ischemic strokes in young adults, imposing a significant socioeconomic burden. However, the source of embolus has been specifically investigated in a few number of studies. In this report, We document the first case of a cryptogenic stroke associated with a pulmonary arteriovenous fistula (PAVF), providing evidence of mural thrombus formation within the PAVF vessel.

Novel three-dimensional porous polyethyleneimine-functionalized graphene-sodium alginate-based aerogel for rapid extraction and sensitive detection of F-LCMs in human serum.

Liquid crystal monomers (LCMs), as essential components of liquid crystal displays, have been identified as potentially hazardous to human health. However, research on highly sensitive methods for assessing internal exposure levels within populations remains limited. This gap in research hinders effective monitoring, early intervention, and the mitigation of health risks associated with LCMs exposure.

Radiomic phenotype of peri-coronary adipose tissue as a potential non-invasive imaging tool for detecting atrial fibrillation.

Objectives：: Epicardial adipose tissue (EAT) contribute to atrial fibrillation (AF). We sought to explore the role of FAI (fat attention index), volume, fat radiomic profile (FRP) from peri-coronary artery adipose tissue (PCAT) on coronary computed tomography angiography (CCTA) in determining the presence of AF and differentiating AF types.

Methods: This study enrolled 300 patients underwent CCTA retrospectively and divided them into AF (n = 137) and non-AF groups (n = 163).

Elevated CD10 neutrophils correlate with non-response and poor prognosis of CD19 CAR T-cell therapy for B-cell acute lymphoblastic leukemia.

Background: The primary challenges in CD19-specific chimeric antigen receptor T-cell (CD19 CAR T) therapy for patients with refractory/relapsed B-cell acute lymphoblastic leukemia (r/r B-ALL) are non-response and relapse; it is urgent to reveal these mechanisms. Neutrophils play a critical role in the immunosuppressive tumor microenvironment (TME), which can hinder CAR T efficacy. Our previous research identified a subset of immunosuppressive neutrophils with a special phenotype (CD14CD10CD45HLA-DRSSC, termed CD10 neuts), which suppress T cell function.

Effect of herpes zoster vaccine on patients after hematopoietic stem cell transplantation: a systematic review and meta-analysis.

Background: Herpes zoster(HZ), a severe complication following hematopoietic stem cell transplantation (HSCT), is associated with significant morbidity. The effect of herpes zoster vaccine(HZV) for preventing HZ on patients after HSCT is unclear. We conducted a systematic review and meta-analysis investigating the efficacy and safety of HZV in HSCT recipients.

CdtB Triggers Colonic Mucosal Barrier Disruption in Mice via Epithelial Tight Junction Impairment Mediated by MLCK/pMLC2 Signaling Pathway.

() has been demonstrated to have clinical relevance to the development of colitis in rodents. produces cytolethal distending toxins (CDTs), which are identified as the most important virulence factors to the pathogenicity of CDT-producing bacteria in animals. However, the precise relationship between CDTs of and intestinal barrier dysfunction remains unclear.

After-School Behaviors, Self-Management, and Parental Involvement as Predictors of Academic Achievement in Adolescents.

How adolescents' after-school behaviors, self-management, and parental involvement are associated with their academic success and personal development has drawn growing attention. This study systematically examined how these three factors predict academic achievement. Data were collected from 353 seventh-grade students through a self-designed daily behavior log sheet and self-management app, and multiple linear regression analysis was conducted to identify key predictors.

Rapid and On-Site Approaches for Determination of Polycyclic Aromatic Hydrocarbons in Water and Air by Surface-Enhanced Raman Spectroscopy.

Polycyclic aromatic hydrocarbons (PAHs) represent a class of carcinogenic, teratogenic, and mutagenic aromatic organic pollutants that are ubiquitous in the environment. The rapid and on-site detection of PAHs remains a challenge. This study proposes point-of-use (POU) surface-enhanced Raman spectroscopy (SERS)-based strategies for the qualitative and quantitative analyses of PAHs in environmental water and air.

Constructing a Homogeneous Medium Layer To Promote the Direct Regeneration of Spent Lithium Iron Phosphate.

With the massive application of lithium-ion batteries in electric vehicles, spent lithium iron phosphate (LFP) batteries have accumulated in recent years, inducing an urgent requirement in recycling technology. Direct repair technology has been considered as a promising approach for recycling spent LFP. However, the traditional solid-phase repair technology is limited by the heterogeneous contact between the lithium source and spent LFP.

Self-Manipulating Sodium Ion Gradient-Based Endogenic Electrical Stimulation Dressing for Wound Repair.

Endogenous electric field (EF) originating from differences in ionic gradients plays a decisive role in the wound healing process. Based on this understanding, a self-manipulating sodium ion gradient-based endogenic electrical stimulation dressing (smig-EESD) is developed to achieve passive, non-invasive, endogenic electrical stimulation of wounds, which avoids the side effects of electrode occupancy, electrochemical reactions, and thermal effects present in traditional exogenous electrical stimulation. smig-EESD reduced the potential at the center of the wound by specifically absorbing Na+ in the exudate, ultimately strengthening the wound endogenous EF.

Gp350-targeted CAR-T therapy in EBV-positive Burkitt lymphoma: pre-clinical development of gp350 CAR-T.

Background: Epstein-Barr virus (EBV) is an oncovirus belonging to the herpesvirus family, associated with the pathogenesis of multiple malignancies, particularly Burkitt lymphoma (BL). The virus remains latent in host cells and plays a critical role in tumor progression through various mechanisms. A key glycoprotein, gp350, expressed during the lytic phase of EBV, is instrumental in viral entry into B cells and presents a unique antigenic target, making it a promising candidate for immunotherapeutic approaches, such as chimeric antigen receptor T-cell (CAR-T) therapy.

Patent foramen ovale-induced retinal artery occlusion: Evidence for in situ Thromobosis.

Background: To report one young man suffered from retinal artery occlusion (RAO) due to patent foramen ovale (PFO).

Objective: The aim of this study was to assess the mechanism for RAO in a patient with a PFO.

Method: A 35-year-old male who developed RAO was studied.

Association of immunosuppressive CD45CD33CD14 CD10HLA-DR neutrophils with poor prognosis in patients with lymphoma and their expansion and activation through STAT3/arginase-1 pathway .

Objective: This study aimed to explore the clinical significance of CD45CD33CD14CD10HLA-DR neutrophils (Cluster of Differentiation 10 [CD10] neutrophils) in B-cell non-Hodgkin's lymphoma (B-NHL). An amplification system of CD10 neutrophils was constructed using cytokines, and the mechanisms underlying the cytokine-induced expansion and activation of the CD10 neutrophil subpopulation were investigated.

Material And Methods: We identified a novel suppressive cell population known as CD10 neutrophils in the peripheral blood of patients with B-NHL in different statuses by flow cytometry and found it to be correlated with interleukin-6 levels, T cell counts, and plasma arginase-1 (Arg-1) levels.

Janus Bound States in the Continuum with Asymmetric Topological Charges.

We propose a novel topological defect called Janus bound states in the continuum (BICs), featuring asymmetric topological charges in upward and downward radiation channels. Our approach involves a photonic crystal slab (PCS) that initially exhibits both out-of-plane and in-plane mirror symmetry, and this PCS possesses one BIC at the Γ point and two BICs off the Γ point. By introducing certain perturbations that break the out-of-plane mirror symmetry, the two off-Γ BICs decompose into four circularly polarized states (C points) with identical topological charges (each with half the topological charge of the original BIC) while the at-Γ BIC is preserved.

Tumor-associated macrophages drive heterogenetic CD10 cancer stem cells to implement tumor-associated neutrophils reprogramming in oral squamous cell carcinoma.

Tumor-associated macrophages (TAMs) in the tumor microenvironment (TME) widely participate in the malignant progression in cancer. Previously, we have demonstrated that M1-like TAMs cascaded a stem-like phenotype of oral squamous cell carcinoma (OSCC). Yet, the underlying mechanisms still need to be demonstrated for the regulation of TAMs on cancer stem cells (CSCs) in OSCC.

Systematic Analysis of E3 Ligase-Related Genes Identified UBE2L3 as a Prognostic Biomarker Associated With Drug Resistance in Acute Myeloid Leukemia.

Background: Acute Myeloid Leukemia (AML) is a prevalent neoplastic disorder. The roles of E3 ubiquitin ligases and related genes in AML drug resistance and prognosis remain unclear.

Methods: Genes were identified from GeneCards and UniProt databases, differentially expressed genes were selected based on transcriptional sequencing data from wild-type and Adriamycin-resistant HL60 (HL60/WT & HL60/ADR) cell lines, and the intersection of these three sources was taken.

The Negative Role of Proton Insertion on the Lifetime of Vanadium-Based Aqueous Zinc Batteries.

Vanadium oxides are attracted cathodes for aqueous zinc batteries owing to their high capacity. However, the limited cyclability of vanadium-based oxide cathodes, especially at low current densities, impedes their practical application. Here, it is revealed that proton insertion is responsible for the limited lifetime of vanadium oxides.

Draft Genome of Naganishia uzbekistanensis from a Clinical Pulmonary Infection.

This study presents the first high-quality assembled genome of Naganishia uzbekistanensis, derived from a clinical isolate CY11558 obtained from a patient with a postoperative pulmonary infection. This work provides an improved reference assembly for downstream research and diagnosis of infections caused by this species.

Eco-friendly synthesis of Arabinoxylan-Catechin conjugates: Enhanced functional and antioxidant properties via cold plasma and ultrasonic methods.

Arabinoxylan-catechin complexes were synthesized using two eco-friendly methods: cold plasma and ultrasonic treatments. Their physicochemical, structural and functional properties were evaluated. Cold plasma and ultrasonic treatments did not significantly alter the molecular structure of arabinoxylan, although a decrease Mw was noticed.

pH-Regulated catechol-modified sodium alginate hydrogel with anti-freezing and high toughness for wearable strain sensor.

Hydrogel-based flexible electronic devices have garnered significant attention due to their excellent mechanical properties, high electrical conductivity, and signal sensitivity. Nevertheless, internal water molecules crystallize inevitably at low temperatures, impairing the performance of hydrogels. Designing anti-freezing and tough hydrogels to meet long-term stability requirements is extremely challenging.

Identification of CDKN3 overexpression as a marker of poor prognosis and potential therapeutic target in low-grade glioma.

Low-grade glioma (LGG) is a primary, slow-growing brain tumor; however, its treatment and prognosis remain challenging. In this study, we analyzed cancer data from the TCGA database, focusing particularly on the expression of the CDKN3 gene in LGG. The results showed that high CDKN3 expression in LGG patients was significantly associated with poor survival outcomes.

LIMA1 Is a Prognostic Senescence-Inhibitory Gene in Head and Neck Squamous Carcinoma.

Background: This study aimed to investigate potential cellular senescence inhibitory genes (CSIGs) and discover novel therapeutic targets in head and neck squamous cell carcinoma.

Methods: Dysregulated CSIGs were identified based on The Cancer Genome Atlas (TCGA) and the Human Aging Genomic Resources (HAGR) database. Prognostic value and immune infiltration were assessed through bioinformatic analysis.

In vitro study of the pro-apoptotic mechanism of amino acid Schiff base copper complexes on anaplastic thyroid cancer.

In the endocrine system, anaplastic thyroid cancer (ATC) is extremely aggressive since it inhibits the majority of medications and treatments. Therefore, there is an immediate demand to identify new treatment approaches or drugs to deal with ATC. Recently, amino acid Schiff base copper complexes have received great attention due to their excellent anti-tumor activity.