Self-Initiated Nano-Micelles Mediated Covalent Modification of mRNA for Labeling and Treatment of Tumors.

As a promising gene therapy strategy, controllable small molecule-mRNA covalent modification in tumor cells could be initiated by singlet oxygen (O) to complete the modification process. However, in vivo generation of O is usually dependent on excitation of external light, and the limited light penetration of tissues greatly interferes the development of deep tumor photo therapy. Here, we constructed a tumor-targeting nano-micelle for the spontaneous intracellular generation of O without the need for external light, and inducing a high level of covalent modification of mRNA in tumor cells.

Gelatinase-responsive biodegradable targeted microneedle patch for abscess wound treatment of S. aureus infection.

Abscess wound caused by bacterial infection is usually difficult to heal, thus greatly affect people's quality of life. In this study, a biodegradable drug-loaded microneedle patch (MN) is designed for targeted eradication of S. aureus infection and repair of abscess wound.

Risk Factors for Thirty-Day Readmission Following Lumbar Surgery: A Meta-Analysis.

Objective: Thirty-day readmission is one of the common complications after lumbar surgery. More 30-day readmission increases the total hospitalization, economic burden, and physical pain of patients, delays the progress of postoperative rehabilitation, and even lead to die. Therefore, it is necessary to analyze the risk factors of 30-day readmission following lumbar surgery.

[Pollution Characteristics and Risk Assessment of Heavy Metals in the Bioretention Systems of Sponge Cities].

As an important water purification and seepage measure for sponge cities, biofiltration systems have been widely used in their construction in China. In order to identify the heavy metal accumulation, pollution, and its potential environmental risk in the biofiltration systems, this study examined the heavy metal contents and spatial distribution characteristics by taking the biofiltration systems of Yuelai new town, Chongqing, the first demonstration area of sponge city construction in China, as the research object, and conducted a risk evaluation of the pollution level and ecological environment in this new town using the contamination factor (CF), geo-accumulation Index (), and potential ecological risk coefficient (PERC). The results showed that, except for Mn, the average contents of Cu, Zn, Pb, Ni, and Cd in the biofiltration systems of Yuelai new town were 4.

Cardio-Cerebrovascular Disease is Associated With Severity and Mortality of COVID-19: A Systematic Review and Meta-Analysis.

At present, COVID-19 is raging all over the world. Many comorbidities, such as diabetes mellitus (OR = 2.67, 95% CI = 1.

An anti-inflammatory isoflavone from soybean inoculated with a marine fungus C23-3.

A new isoflavone derivative compound (psoralenone) was isolated from soybean inoculated with a marine fungus C23-3, together with seven known compounds including isoflavones -, butyrolactone I () and blumenol A (). Their structures were elucidated by MS, NMR, and ECD. Psoralenone displayed moderate anti-inflammatory activity in the LPS-induced RAW264.

Butyrolactone-I from Coral-Derived Fungus Attenuates Neuro-Inflammatory Response via Suppression of NF-κB Pathway in BV-2 Cells.

Butyrolactone-I (ZB5-1) from the coral-derived fungus was investigated in this study to estimate its anti-neuroinflammatory effects on lipopolysaccharide (LPS)-induced BV-2 microglia cells. MTT assay indicated that ZB5-1 in tested concentrations had no cytotoxicity on BV-2 cells, and significantly reduced the production of nitric oxide (NO), measured using Griess reagent, and interleukin-1 beta (IL-1β), detected by enzyme-linked immunosorbent assay (ELISA). ZB5-1 also down-regulated the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dose-dependent manner by Western blot analysis.

The study of perceived stress, coping strategy and self-efficacy of Chinese undergraduate nursing students in clinical practice.

The aim of the study was to explore the coping strategy and the effects of self-efficacy of Chinese undergraduate nursing students when they face the stress in clinical practice. Convenience sampling was used to recruit undergraduate nursing students in Mainland China who have practiced 3 months in hospitals in their final college year. Self-report questionnaires including demographics, Perceived Stress Scale, coping behaviour inventory and Generalized Self-Efficacy Scale were collected.

[Secondary metabolites of seaweed endophytic fungi ZJ27 in the South China Sea coast].

Objective: To study the secondary metabolites of coastal seaweed endophytic fungi ZJ27.

Methods: The compounds were isolated by chromatographic technique. The structures were identified by comprehensive physic-chemical properties and spectral methods.

[Immunoloregulation effect of polysaccharide isolated from octopus dollfusi on mouse].

Objective: To study the immunoloregulation effect of three polysaccharides OGI, OG2and OG3 extracted from Octopus dollfusi muscle, gonad, digestive gland, respectively.

Methods: Spleen cell proliferation was measured by MTT assay and index of immune organs were weighed and calculated.

Results: OGI and OG2 could increase the proliferation of mouse spleen cell of normal mice, and significantly increased the proliferation of the spleen of immunosuppression mice caused by sccyclophosphamide, while showed no cooperation with ConA; OG3 appeared suppression for the two spleens.

[Solid-phase microextraction for the analysis of drugs and biological samples].

Solid-phase microextraction (SPME) as a new sample pretreatment technique was developed in 1990s. The applications of SPME on drugs and biological samples are reviewed with 60 references. The references were classified into twelve groups according to the character of analysis.