Oncogenic and microenvironmental signals drive cell type specific apoptosis resistance in juvenile myelomonocytic leukemia.

Juvenile myelomonocytic leukemia (JMML) is caused by constitutively activated RAS signaling and characterized by increased proliferation and predominant myelomonocytic differentiation of hematopoietic cells. Using MxCre;Ptpn11 mice, which model human JMML, we show that RAS pathway activation affects apoptosis signaling through cell type-dependent regulation of BCL-2 family members. Apoptosis resistance observed in monocytes and granulocytes was mediated by overexpression of the anti-apoptotic and down-regulation of the pro-apoptotic members of the BCL-2 family.

Organizational Leaders' Views on Digital Health Competencies in Medical Education: Qualitative Semistructured Interview Study.

Background: Digital technologies (DTs) have profoundly impacted health care delivery globally and are increasingly used in clinical practice. Despite this, there is a scarcity of guidelines for implementing training in digital health competencies (DHC) in medical schools, especially for clinical practice. A lack of sustained integration of DHC risks creating knowledge gaps due to a limited understanding of how DT should be used in health care.

The Landscape of Supportive Care Needs Among Prostate Cancer Patients in New Zealand: A Cross-Ethnic Analysis.

Background: Prostate cancer (PCa) is a significant health burden within New Zealand (NZ). Survival gains from prostate cancer have created a shift in focus from survival towards quality of life (QoL) and supportive care during extended survivorship.

Method: We launched a nation-wide cross-sectional survey and recruited three cohorts of 1000 men with prostate cancer (men diagnosed with prostate cancer within 1 year, between 1 and 3 years, and between 3 and 5 years) as well as an additional Māori men group (N = 4000 in total).

Tailoring the special hole transfer layer between BiVO and oxygen evolution co-catalysts interfaces for boosting photoelectrochemical water splitting.

Tailoring the hole transport layer (HTL) between BiVO (BVO) and oxygen evolution co-catalysts (OECs) interfaces is a leading strategy to improve the performance of photoelectrochemical (PEC) water splitting. Nevertheless, the limited driving force at the BVO/OECs interfaces severely hinders the transport of charge carriers. In this study, we designed a specialized defective transition metal oxide (Vo-MnO) as the HTL.

Construction and validation of a risk prediction model for postoperative frailty in older adults:a multicenter study.

Background: Postoperative frailty is an important determinant of postoperative recovery and survival outcomes. Predicting the onset of postoperative frailty is significant importance for the rehabilitation of the elderly people after surgery. Our study aims to develop and evaluate a predictive model for postoperative frailty on the 30 day in elderly patients.

Detecting Glaucoma in Highly Myopic Eyes From Fundus Photographs Using Deep Convolutional Neural Networks.

Background: High myopia (HM) is a major risk factor for glaucoma. However, glaucomatous optic neuropathy is often undiagnosed owing to atypical structural alterations with axial elongation. Moreover, an algorithm to detect glaucoma in highly myopic eyes has not yet been reported.

Short IL-18 generated by caspase-3 cleavage mobilizes NK cells to suppress tumor growth.

Interleukin (IL)-18 functions primarily through its 18-kDa mature form produced from caspase-1 cleavage. However, IL-18 can also be processed by other proteases, leading to the generation of different fragments with less recognized functions. Here, we discover that, in cancer cells, caspase-3 cleavage of IL-18 generates a 15-kDa form of IL-18, referred to as short IL-18.

SAA3 deficiency exacerbates intestinal fibrosis in DSS-induced IBD mouse model.

Intestinal fibrosis, as a late-stage complication of inflammatory bowel disease (IBD), leads to bowel obstruction and requires surgical intervention, significantly lowering the quality of life of affected patients. SAA3, a highly conserved member of the serum amyloid A (SAA) apolipoprotein family in mice, is synthesized primarily as an acute phase reactant in response to infection, inflammation and trauma. An increasing number of evidence suggests that SAA3 exerts a vital role in the fibrotic process, even though the underlying mechanisms are not yet fully comprehended.

Weight Status and Myopia in Children and Adolescents: A Nationwide Cross-Sectional Study of China.

Background/objectives: The prevalence of both myopia and obesity is increasing among children and adolescents around the world. We aimed to examine the association between weight status and myopia in Chinese children and adolescents.

Methods: The analysis included 35,108 participants aged 6-17 from a nationwide cross-sectional survey.

Quantitative Analysis of Phosphorothioate Isomers in CRISPR sgRNA at Single-Residue Resolution Using Endonuclease Digestion Coupled with Liquid Chromatography Cyclic Ion Mobility Mass Spectrometry (LC/cIMS).

Phosphorothioate (PS) modifications in single-guided RNA (sgRNA) are crucial for genome editing applications using the CRISPR/Cas9 system. These modifications may enhance sgRNA stability, pharmacokinetics, and binding to targets, thereby facilitating the desired genetic alterations. Incorporating multiple PS groups at varying positions may introduce chiral centers into the sgRNA backbone, resulting in a complex mixture of constitutional- and stereoisomers that challenges current analytical capabilities for reliable identification and quantification.

Exploring the mechanisms influencing psychological adaptation in athletes in high-risk sports: a moderated mediation model.

This study explores the impact of state and trait anxiety on perceived stress in athletes in high-risk sports, focusing on the mediating role of cognitive reappraisal and the moderating effect of sports psychological skills. Data were collected from athletes in sports like Boxing, Sanda, Mixed Martial Arts, Freestyle Skiing, and Ski Jumping. The analysis, conducted using the PROCESS macro revealed that both state and trait anxiety significantly predict perceived stress (β = 0.

Infrared Electrochemiluminescence from A Water-Soluble Anion-π Emitter for Sensitive Perfluorooctanoic Acid Sensing.

Electrochemiluminescence (ECL) analysis stands out among various analytical methods due to its exceptional sensitivity and accuracy. However, the poor solubility of most ECL probes limits their effectiveness in aqueous environments. To address this challenge, we developed a water-soluble anion-π ECL luminophore, DPBC-OTS.

CMPK2 promotes NLRP3 inflammasome activation via mtDNA-STING pathway in house dust mite-induced allergic rhinitis.

Background: House dust mite (HDM) is the leading allergen for allergic rhinitis (AR). Although allergic sensitisation by inhaled allergens renders susceptible individuals prone to developing AR, the molecular mechanisms driving this process remain incompletely elucidated.

Objective: This study aimed to elucidate the molecular mechanisms underlying HDM-induced AR.

Simultaneous determination of twelve microcystins in algal supplements on the Chinese market and possible risks to exposed populations.

Algae supplements are widely recognized for their nutritional benefits and are commonly marketed as natural health products. However, concerns regarding contamination with cyanobacterial toxins have been raised. Moreover, there is very little data regarding the potential contamination of algal supplements on the Chinese market by these toxins.

Self-assembled materials with an ordered hydrophilic bilayer for high performance inverted Perovskite solar cells.

While self-assembled material based inverted perovskite solar cells have surpassed power conversion efficiencies of 26%, enhancing their performance in large-area configurations remains a significant challenge. In this work, we report a self-assembled material based hole-selective layer 4-(7H-dibenzo[c,g]carbazol-7-yl)phenyl)phosphonic acid, with a π-expanded conjugation. The enhanced intermolecular π-π interactions facilitate the self-assembly of 4-(7H-dibenzo[c,g]carbazol-7-yl)phenyl)phosphonic acid molecules to form an ordered bilayer with a hydrophilic surface, which passivates the buried perovskite interface defect and enables high-quality and large-area perovskite preparation, while simultaneously enhancing interfacial charge extraction and transport.

Predicting Source Rock Distribution of the Ninth Member of the Upper Triassic Yanchang Formation Based on Well-Logging Parameters and TOC Values in the Longdong Area, Ordos Basin, China.

In recent years, new oil reservoirs have been discovered and exploited in the ninth member (Chang 9 Member, Ty) of the Upper Triassic Yanchang Formation (Ty) in the Longdong area, Ordos Basin. Some studies have shown that the crude oils of the Chang 9 Member may originate from the Chang 9 source rock in some areas, which may be related to the distribution of the source rock. However, the distribution of the Chang 9 source rock in the Longdong area is still unclear, which hinders further exploration and development of petroleum.

Association between plasma trimethylamine N-oxide and cerebral white matter hyperintensity: a cross-sectional study.

Background: Cerebral white matter hyperintensity (WMH) is a pivotal imaging feature of cerebral small vessel disease (CSVD), closely correlated with an elevated risk of ischemic stroke (IS). Trimethylamine N-oxide (TMAO), a metabolite of gut microbiota, is increasingly associated with IS and atherosclerosis. However, the intricate relationship between TMAO and WMH remains ambiguous.

Energy restriction inhibits β-catenin ubiquitination to improve ischemic stroke injury via USP18/SKP2 axis.

Ischemic stroke (IS) remains a global health issue because of its great disability and mortality. Energy restriction (ER) has been justified to perform an inhibitory role in cerebral injury caused by IS. This research was purposed to inquire the potential molecular mechanism of ER in IS.

The effect of integrated medical care on the daily life of patients with coronary heart disease.

This study aims to explore the impact of comprehensive medical care on the daily life of patients with coronary heart disease (CHD) and to evaluate its effectiveness in improving quality of life, alleviating symptoms, and reducing the risk of cardiac events. A new comprehensive medical care scheme combining Traditional Chinese Medicine nursing differentiation, collaborative nursing interventions, and specialized community care was proposed. Patients with CHD were recruited as study subjects.

Predictive value of ig Mycoplasma pneumoniae-DNA, high-density lipoprotein, natural killer cell, and platelet levels for diagnosing severe M. pneumoniae pneumonia in children.

Objective: The present study aimed to assess the predictive value of Ig Mycoplasma pneumoniae (MP)-DNA, high-density lipoprotein (HDL), natural killer (NK) cell, and platelet (PLT) levels for the diagnosis of severe MP pneumonia (SMPP) in children with MP pneumonia (MPP).

Methods: Children with MPP admitted to our hospital from August 2022 to February 2024 were selected and assigned to the non-SMPP (NSMPP) and SMPP groups according to whether they had severe pneumonia. The following parameters were analyzed and compared between the two groups by the rank-sum test: age; Ig MP-DNA level; white blood cell, neutrophil (N), and monocyte counts; platelet (PLT), C-reactive protein (CRP), lactate dehydrogenase (LDH), triglycerides, high-density lipoprotein (HDL), low-density lipoprotein, and procalcitonin levels; and levels of T cells, CD4 T cells, CD8 T cells, B cells, and NK cells.

Circulating Tumor cells and multiple indicators combined to identify the risk of poorer prognosis in patients with resected non-small cell lung cancer.

Background: Surgical resection is an important treatment option for patients with non-small cell lung cancer (NSCLC). However, recurrence and survival rates remain a cause of concern. To further improve prognosis, more studies have focused on liquid biopsy, which has significant value as a prognostic factor for defining the risk stratification of postoperative NSCLC patients.

Efficacy and safety of minocycline in retinitis pigmentosa: a prospective, open-label, single-arm trial.

Retinitis pigmentosa (RP) is characterized by progressive photoreceptor cells death accelerated by the proliferation and activation of microglia pathologically. No consensus exists on the treatment. Minocycline is recognized as a microglia inhibitor with great anti-inflammatory and neuro-protective functions.

Molecular characteristics and prognostic role of EPHA2 in human tumors via pan-cancer analysis.

The tyrosine kinase ephrin type-A receptor 2 (EPHA2) was remarkably elevated expressed in various tumors and plays a crucial role in cancer tumorigenesis and progression, while pan-cancer analyses are currently lacking. This study was designed to analyze the expression status and prognostic significance of EPHA2 in pan-cancer. By mining The Cancer Genome Atlas data, we performed a comprehensive and systematic characterization of EPHA2 across >10,000 samples of 33 types of cancer.