Unveiling the Differences in Biological Properties of Dental Pulp Stem Cells from Normal and Inflamed Pulp: A Comprehensive Comparative Study.

BACKGROUND The aims of the study were to comprehensively compare the morphology, immunophenotype, proliferation, migration, and regeneration potential of normal dental pulp stem cells (DPSCs) versus inflammatory dental pulp stem cells (iDPSCs). MATERIAL AND METHODS Healthy pulp or inflamed pulp tissue was used to isolate and culture DPSCs and iDPSCs, respectively. These cell populations were characterized by flow cytometry, colony formation assay, transwell assay, and multi-directional differentiation in vitro.

Effect of acidic culture conditions on the proliferation, apoptosis, and migration ability of human tongue squamous cell carcinoma cells and its related mechanism.

Objectives: This study aims to explore the effect of acidic culture conditions on the proliferation, apoptosis, and migration ability of human tongue squamous cell carcinoma SCC15 and CAL27 cells and its potential molecular mechanism.

Methods: After acidic culture for different periods, methyl thiazolyl tetrazolium (MTT) method was adop-ted to detect the cell proliferation of SCC15 and CAL27. Flow cytometry was employed to detect the apoptosis level of SCC15 and CAL27 cells.

FKBP11 promotes cell proliferation and tumorigenesis via p53-related pathways in oral squamous cell carcinoma.

Oral squamous cell carcinoma (OSCC) is one of the causes of cancer-related death worldwide. The abnormal proliferation ability of OSCC has become one of the major reasons for its poor prognosis. FK-506 binding protein 11 (FKBP11) is abnormally expressed in malignant tumors and affects many biological processes.

[Ex vivo assessment of the potency of optical coherence tomography for the detection of early occlusal caries].

Objective: This study aimed to evaluate the potency of optical coherence tomography (OCT) to detect early occlusal caries compared with clinical visual examination.

Methods: Approximately 97 sites of occlusal fissures on 77 extracted accessional human teeth were scored by three examiners using conventional visual examination and OCT. Results of histological examination on these sites obtained by polarimicroscope served as a gold standard to analyze the sensitivity (SE), specificity (SP), positive predictive value (PPV), and negative predictive value (NPV).

The correlation between pancreatic steatosis and metabolic syndrome in a Chinese population.

Background: Type 2 diabetes mellitus, obesity and hepatic steatosis showed a strong correlation with metabolic syndrome. However, data on the influence of pancreatic steatosis on metabolic syndrome are lacking.

Objective: Our aim is to perform the prevalence of pancreatic steatosis in adults and its association with metabolic syndrome in a Chinese population.

Effects of roads on Castanopsis carlesii seedlings and their leaf herbivory in a subtropical forest in China.

The effects of a forest road on Castanopsis carlesii (Hemsley) Hayata (Fagales: Fagaceae) seedlings and their leaf herbivory were investigated in a subtropical forest at Jiulianshan National Nature Reserve, Jiangxi, China. A total of 1124 seedlings, 33949 leaves, 468 leaf mines, and 205 leaf galls were found. Generally, individual numbers, tree heights, and leaf numbers of C.

[Effect of yanggan yishui granule on collagen I, III, and IV, and FN in spontaneously hypertensive rats].

Objective: To observe the effect of Yanggan Yishui Granule (YGYSG) on collagen protein I, III, and IV, as well as fibronection (EN) in spontaneously hypertensive rats (SHR), and to explore its possible renal protective mechanisms.

Methods: Fourty SHR were randomly divided into four groups, i.e.

Circular distribution pattern of plant modulars and endophagous herbivory within tree crowns: the impact of roadside light conditions.

The circular distributions of plant modulars (branches, leaves) and endophagous herbivory (mines, galls) were investigated within the crowns of four dominant Fagaceae trees in a subtropical evergreen broadleaf forest at Jiulianshan National Nature Reserve, Jiangxi, China. The hypothesis is that more plant modulars and more endophagous herbivory should occur in the crown area perpendicular to the roads. Circular statistical techniques were used to verify new patterns of the impact of roads on plants and insects.

Contribution of growth differentiation factor 6-dependent cell survival to early-onset retinal dystrophies.

Retinal dystrophies are predominantly caused by mutations affecting the visual phototransduction system and cilia, with few genes identified that function to maintain photoreceptor survival. We reasoned that growth factors involved with early embryonic retinal development would represent excellent candidates for such diseases. Here we show that mutations in the transforming growth factor-β (TGF-β) ligand Growth Differentiation Factor 6, which specifies the dorso-ventral retinal axis, contribute to Leber congenital amaurosis.

Velocity vector imaging of longitudinal mechanical properties of upstream and downstream shoulders and fibrous cap tops of human carotid atherosclerotic plaque.

Aims: Atherosclerotic plaque rupture is closely related to high regional mechanical stress in the plaque itself. We aimed to explore the longitudinal mechanical properties of upstream and downstream shoulders and fibrous cap tops of human atherosclerotic plaques in vivo by velocity vector imaging (VVI) combined with acoustic densitometry (AD) imaging.

Methods And Results: We included 135 patients with carotid atherosclerotic plaque.

[Effect of yanggan yishui granule on plasma Ang II, TGF-beta1, CTGF content, and renal cortical Ang II , TGF-beta1, and CTGF mRNA expressions in spontaneously hypertensive rats].

Objective: To study the effect of Yanggan Yishui Granule (YGYSG) on 12-week-old spontaneously hypertensive rats (SHR) on plasma angiotensin II (Ang II), transforming growth factor beta1 (TGF-beta1), connective tissue growth factor (CTGF) content and renal cortical Ang II, TGF-beta1, CTGF mRNA expressions, and to further explore the mechanism of YGYSG in the kidney protection.

Methods: Forty 12-week-old male SHR were randomized into the model group, the Bella Plymouth group (at the daily dose of 1.8 mg/kg), the YGYSG low dose group (at the daily dose of 5.

[Effects of acid and heating treatments on the structure of sepiolite and its adsorption of lead and cadmium].

The effects of acid and heating treatment on the structure of sepiolite and its adsorption capacities for Pb2+ and Cd2+ were investigated using X-ray powder diffraction, infrared spectroscopy, surface area analyses and batch sorption experiments in this paper. The results showed that the BET surface area of the sepiolite samples grew with increase concentrations of HCl and prolong treatment time and no obvious structural changes were observed. CaCO3 as the impurity in the natural sepiolite can not be removed completely in the treatment process with 0.

[Effect of calmodulin antagonist O-4-ethoxyl- butyl-berbamine on the proliferation of human breast cancer cell line MCF-7 and its relevant mechanism].

Objective: To investigate the effect of calmodulin antagonist O-(4-ethoxyl-butyl)-berbamine (EBB) on proliferation of human breast cancer cell MCF-7 and its possible mechanism.

Methods: MTT assay was used to analyze the effect of EBB on tumor cells growth. Flow cytometry was used to detect its impact on the cell cycle of MCF-7 cells.

[The safety and outcome of patients with acute myocardial infarction transferred for primary angioplasty].

Objective: To evaluate the safety and outcome of patients with acute myocardial infarction (AMI) transferred for primary percutaneous coronary intervention (PCI).

Methods: Data from patients with ST elevation AMI urgently transferred from first admitted hospitals to our cath-lab to receive primary PCI were analyzed. According to time intervals from symptom onset to transfer, the patients were divided into early transfer (< 6 h, n = 26), delayed transfer (6 - 24 h, n = 39) and late transfer (24 h to 1 week, n = 18) group.

A novel genetic locus for familial febrile seizures and epilepsy on chromosome 3q26.2-q26.33.

Febrile seizures (FS) are common in children, and the incidence is 2-5% before the age of 5 years. A four-generation Chinese family with autosomal dominant febrile seizure and epilepsy was studied by genome-wide linkage analysis. Significant linkage was identified with markers on chromosome 3q26.

[Cytogenetic analysis of partial trisomy 9p resulting from a reciprocal balanced 9/21 translocation].

In this study, a four-generation Chinese family in Nanyang, Henan Province was identified with partial trisomy 9p syndrome. Of the 23 family members studied, six were characterized with mental retardation and mild facial and little finger anomalies. All affected family members demonstrated significant intrafamilial homogeneous phenotype except concomitance epilepsy in proband.

[Multicentric randomized double blinded clinical study of Yiqi Tongmai Oral Liquid against angina pectoris in patients with coronary heart disease].

Objective: To study the efficacy and safety of Yiqi Tongmai Oral Liquid (YQTM), a traditional compound Chinese herbal medicine, in treating angina pectoris in patients with coronary heart disease.

Methods: A multicentric, randomized, double blinded and paralleled controlled trial was conducted on 110 patients in trial group treated with YQTM, and 109 patients in control group treated with Shuxin Oral Liquid (SX). Cure and effective rates in both groups were evaluated.

[The relationship between abnormity of synaptonemal complex and male fertility impairment in human].

It was said that 11% of all men showed their infertility. The genetic origins of male infertility may be classified into three main groups: chromosome abnormalities, microdeletions and gene mutations. Growing literature has shown that spermatogenesis failure or reproductive failure (pregnancy wastages) occurred in male carriers of chromosomal distortion/aberration.