Effect of Rac1 downregulation mediated by shRNA on the biological behaviour of human cervical cancer cells.

Objective: The function of Ras-related C3 botulinum toxin substrate1 (Rac1) in the progression of cervical cancer is unclear. This study used RNA interference technology to explore the involvement of Rac1 in the regulation of cervical cancer cells.

Methods: A short hairpin (sh) RNA plasmid targeting Rac1 was constructed and transfected into HeLa cells.

[Follow-up of pediatric inflammatory bowel disease].

Objective: To analyze the clinical characteristics and prognosis of pediatric inflammatory bowel disease (IBD) through a long-term follow-up so as to improve the diagnosis and management of IBD in children.

Methods: Seventy-three IBD patients admitted into our hospital from May 2000 to September 2010 were re-evaluated with the uniform diagnostic criteria proposed by the 2010 consensus diagnostic criteria for pediatric IBD. All patients were followed up by questionnaire, telephone and face-to-face interview.

Tapering duodenoplasty and gastrojejunostomy in the management of idiopathic megaduodenum in children.

Purpose: The purpose of this study is to present the management of idiopathic megaduodenum in children.

Methods: A retrospective analysis of 4 cases of megaduodenum admitted from 2005 to 2011 was performed evaluating clinical features, radiologic data, treatment, pathologic findings, and prognosis. The corresponding literature was reviewed.