Full-Length Transcriptome Profile of Revealed by Nanopore Sequencing.

The Asian honey bee () plays a crucial role in providing abundant bee products and in maintaining ecological balance. Despite the availability of the genomic sequence of the Asian honey bee, its transcriptomic information remains largely incomplete. To address this issue, here we constructed three pooled RNA samples from the queen, drone, and worker bees of and performed full-length RNA sequencing using Nanopore single-molecule sequencing technology.

Transcriptome changes of Apis mellifera female embryos with fem gene knockout by CRISPR/Cas9.

The sex of honey bees is decided by a regulatory cascade comprising of csd, fem and Amdsx. In order to further identify other genes involved in sex determination and differentiation of honey bees in the early stages of embryo development, the CRISPR/Cas9 method was used to knock out fem gene in the embryonic stage of diploid western honey bees, and RNA-seq was used to analyze gene expression changes in the embryo after fem knockout. Finally, we found that the bees had undergone gender changes due to fem knockout.

Apelin/APJ-Manipulated CaMKK/AMPK/GSK3 Signaling Works as an Endogenous Counterinjury Mechanism in Promoting the Vitality of Random-Pattern Skin Flaps.

A random-pattern skin flap plays an important role in the field of wound repair; the mechanisms that influence the survival of random-pattern skin flaps have been extensively studied but little attention has been paid to endogenous counterinjury substances and mechanism. Previous reports reveal that the apelin-APJ axis is an endogenous counterinjury mechanism that has considerable function in protecting against infection, inflammation, oxidative stress, necrosis, and apoptosis in various organs. As an in vivo study, our study proved that the apelin/APJ axis protected the skin flap by alleviating vascular oxidative stress and the apelin/APJ axis works as an antioxidant stress factor dependent on CaMKK/AMPK/GSK3 signaling.

Effect of hydraulic retention time, ZVI concentration, and Fe concentration on autotrophic denitrification efficiency with iron cycle bacterium strain CC76.

The immobilized reactor of iron-reducing bacteria and zero-valent iron (ZVI) integrated system was established. This study has shown that the effects of hydraulic retention times (9, 11, 13 h), ZVI concentrations (2, 4, 6, 8 mg/L), and Fe concentrations (5, 10, 15 mg/L) on the denitrification characteristics of iron cycle bacterium strain CC76. The results show that the longer the HRT is, the stronger ability of bacteria to remove nitrate.

The performance and mechanism of simultaneous removal of fluoride, calcium, and nitrate by calcium precipitating strain Acinetobacter sp. H12.

A calcium precipitating and denitrifying bacterium H12 was used to investigate the F removal performance and mechanism. The results showed that the strain H12 reduced 85.24% (0.

High-Efficiency CRISPR/Cas9-Mediated Gene Editing in Honeybee () Embryos.

The honeybee () is an important insect pollinator of wild flowers and crops, playing critical roles in the global ecosystem. Additionally, the honeybee serves as an ideal social insect model. Therefore, functional studies on honeybee genes are of great interest.

[The differential expression of the human lung carcinoma cells infected with high pathogenic avian influenza virus A/Anhui/1/2005 (H5N1)].

Objective: To identify genes in human cells infected with high pathogenic avian influenza viruses H5N1.

Methods: The lung carcinoma cells line A549 was infected with H5N1 and H1N1, respectively. We harvested the infected cells at the different time points after infection and screened the genes with differential expression via microarray technology.

[Generation of neutralizing recombinant human antibodies for targeting highly pathogenic avian influenza A (H5N1) virus].

Two human Fab antibodies against avian influenza A (H5N1) virus were obtained by panning a H5N1 patient-derived antibody phage library using purified virions of the H5N1 patient isolate A/Anhui/1/2005 and HA protein of the H5N1 reference viruse A/Viet Nam/1203/2004. After testing the binding properties and antiviral function to H5N1 virus, the selected Fab antibodies were converted to full human IgG antibodies with recombinant baculovirus/insect cell system. Both mAbs, AVFluIgG01 and AVFluIgG03, bound to HA in immunofluorescence assay (IFA) without cross-reaction with the other substypes of influenza A viruses (H1N1, H3N2).

[Generation of Chicken Germ-line Chimeras by Transferring PGCs and Their Identification by AFLP.].

PGCs (Primordial germ cells) were isolated from the blood of 51~56 h hatching Shiqiza chicken embryos by Ficoll density gradient centrifugation. The PGCs were injected into 2.5 d hatching embryos of H breed chicken to produce germ-line chimeras.