Circle-seq reveals that eccDNA may be a key blood biomarker for HBV-associated liver cancer.

Introduction: Extrachromosomal circular DNA (eccDNA) regulates tumor occurrence and development. Relevant eccDNA profiles have been established for various types of cancer; however, the eccDNA expression profiles in the blood of patients with hepatocellular carcinoma (HCC) and liver cirrhosis (LC) remain unknown. The present study aimed to investigate the eccDNA expression profiles in the blood of patients with HCC and LC.

Simulation of Wire Arc Additive Manufacturing in the Reinforcement of a Half-Cylinder Shell Geometry.

Wire arc additive manufacturing (WAAM) is an additive manufacturing process based on gas metal arc welding. It allows the fabrication of large-volume metal components by the controlled deposition and stacking of weld beads. Next to the near-net-shape manufacturing of metal components, WAAM is also applied in the local reinforcement of structural parts, such as shell geometries.

Comprehensive folding variations for protein folding.

The revelation of protein folding is a challenging subject in both discovery and description. Except for acquirement of accurate 3D structure in protein stable state, another big hurdle is how to discover structural flexibility for protein innate character. Even if a huge number of flexible conformations are known, difficulty is how to represent these conformations.

A New High-throughput Real-time PCR Assay for the Screening of Multiple Antimicrobial Resistance Genes in Broiler Fecal Samples from China.

Objective: Antimicrobial resistance (AMR) has become a global concern and is especially severe in China. To effectively and reliably provide AMR data, we developed a new high-throughput real-time PCR assay based on microfluidic dynamic technology, and screened multiple AMR genes in broiler fecal samples.

Methods: A high-throughput real-time PCR system with an new designed integrated fluidic circuit assay were performed AMR gene detection.

Linc00152 promotes cancer progression in hepatitis B virus-associated hepatocellular carcinoma.

Background: The X protein (HBx) plays as a key role in hepatocarcinogenesis associated with hepatitis B virus (HBV) infections. The study aimed to figure out the role of Linc00152 in hepatocellular carcinoma (HCC) and the association between the expression levels of Linc00152 and HBx.

Methods: QRT-PCR assays were applied to analyzed the expression levels of Linc00152 and HBx.