CircIFNGR2 enhances proliferation and migration of CRC and induces cetuximab resistance by indirectly targeting KRAS via sponging to MiR-30b.

Currently the clinical efficacy of colorectal cancer (CRC) which is the most common malignant tumors over the world has not reached an ideal level. Cetuximab, the monoclonal antibody targeting the extracellular domain of EGFR, has shown its great efficacy in the promotion of apoptosis and the inhibition of tumor cells-like characteristics in numerous cancers. However certain KRAS wild-type CRC patients unexpectedly show cetuximab resistance and the specific mechanism remains unclear.

Dynamic effects of eifferent nail placement strategies on LenkeC scoliosis.

Background: Although the internal fixation technique for scoliosis is effective, there is a great risk of nail placement in actual operation.

Objective: To compare the effects of three different nail placement strategies on LenkeC patients with scoliosis under cyclic loading.

Methods: Firstly, the finite element model was established by using CT scanning images and X-ray images of patients with LenkeC scoliosis.

Discrepancy of QF-PCR, CMA and karyotyping on a de novo case of mosaic isodicentric Y chromosomes.

Background: Isodicentric chromosomes are the most frequent structural aberrations of human Y chromosome, and usually present in mosaicism with a 45, X cell line. Several cytogenetic techniques have been used for diagnosing of uncommon abnormal sex chromosome abnormalities in prenatal cases.

Case Presentation: A 26-year-old healthy woman was referred to our centre at 24 weeks of gestation age.

MiR-452 promotes an aggressive colorectal cancer phenotype by regulating a Wnt/β-catenin positive feedback loop.

Background: Aberrant activation of Wnt/β-catenin signaling pathway is considered to be an important issue in progression and metastasis of various human cancers, especially in colorectal cancer (CRC). MiR-452 could activate of Wnt/β-catenin signaling. But the mechanism remains unclear.

MicroRNA-137, an HMGA1 target, suppresses colorectal cancer cell invasion and metastasis in mice by directly targeting FMNL2.

Background & Aims: Formin-like (FMNL)2 is up-regulated in colorectal tumors and has been associated with tumor progression, but little is known about regulatory mechanisms. We investigated whether microRNAs regulate levels of FMNL2 in colorectal cancer (CRC) cells.

Methods: We used real-time polymerase chain reaction and immunoblot analyses to measure levels of miR-137, high-mobility group AT-hook (HMGA)1, and FMNL2 in CRC cells and tissue samples from patients (n = 50).

Low expression of DAB2IP contributes to malignant development and poor prognosis in hepatocellular carcinoma.

Background And Aim: DOC-2/DAB2 interactive protein gene (DAB2IP) is a novel member of the Ras GTPase-activating protein family and plays a tumor suppressive role in cancer progression, but its function in hepatocellular carcinoma (HCC) remains unclear. This aims of this study were to analyze the clinicopathological features of DAB2IP expression in HCC, and to determine the effect of DAB2IP on HCC cell behaviors in vitro.

Methods: The expression of DAB2IP was detected in hepatocyte cell line and HCC cell lines by real-time reverse transcription-polymerase chain reaction and western blot.

Overexpression of RhoGDI2 correlates with tumor progression and poor prognosis in colorectal carcinoma.

Background: RhoGDI2 has been identified as a regulator of tumor metastasis but its role in cancer remains controversial. The aims of this study were to analyze the function of RhoGDI2 in colorectal carcinoma (CRC), and to determine its possible signaling pathway in CRC.

Methods: The expression of RhoGDI2 was detected in CRC cell lines, and 20 matched pairs of fresh CRC tissues, and 120 cases of clinical paraffin-embedded CRC tissues by real-time RT-PCR, Western blot, RT-PCR, or immunohistochemistry.

Down-regulation of formin-like 2 predicts poor prognosis in hepatocellular carcinoma.

The formin-like 2 protein is a member of the diaphanous-related formin family that controls actin-dependent processes such as cell motility and invasion. This study aimed at clarifying formin-like 2 expression in hepatocellular carcinoma and its correlation with clinicopathologic features and also exploring the effects of formin-like 2 transfection on cell motility and invasion in vitro. Real-time polymerase chain reaction and Western blotting showed that expression of formin-like 2 was lower in 4 hepatocellular carcinoma cell lines than those in normal hepatic epithelial cells (P < .