Small Molecule-Inducible and Photoactivatable Cellular RNA N1-Methyladenosine Editing.

N1-methyladenosine (mA) modification is one of the most prevalent epigenetic modifications on RNA. Given the vital role of mA modification in RNA processing such as splicing, stability and translation, developing a precise and controllable mA editing tool is pivotal for in-depth investigating the biological functions of mA. In this study, we developed an abscisic acid (ABA)-inducible and reversible mA demethylation tool (termed AI-dmA), which targets specific transcripts by combining the chemical proximity-induction techniques with the CRISPR/dCas13b system and ALKBH3.

Isothermal Amplification-Based Detection of Single-Base RNA N6-Methyladenosine.

N6-methyladenosine (mA) has recently gained much attention due to its diverse biological functions. Currently, the commonly used detection methods for locus-specific mA marks are complicated to operate, it is difficult to quantify the methylation level, and they have high false-positive levels. Here, we report a new method for locus-specific mA detection based on the methylate-sensitive endonuclease activity of MazF and the simultaneous amplification and testing (SAT) method, termed "mA-MazF-SAT".

Rolling circle extension-assisted loop-mediated isothermal amplification (Rol-LAMP) method for locus-specific and visible detection of RNA N6-methyladenosine.

N6-methyladenosine (m6A) is the most prevalent RNA modification in eukaryotic mRNAs. Currently available detection methods for locus-specific m6A marks rely on RT-qPCR, radioactive methods, or high-throughput sequencing. Here, we develop a non-qPCR, ultrasensitive, isothermal, and naked-eye visible method for m6A detection based on rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP), named m6A-Rol-LAMP, to verify putative m6A sites in transcripts obtained from the high-throughput data.

A Fenton-Like Nanocatalyst Based on Easily Separated Magnetic Nanorings for Oxidation and Degradation of Dye Pollutant.

In this study, uniform FeO magnetic nanorings (FeO-MNRs) were prepared through a simple hydrothermal method. The morphology, magnetic properties, and structure of the product were characterized by transmission electron microscope (TEM), scanning electron microscope (SEM), high resolution transmission electron microscopy (HRTEM), vibrating sample magnetometer (VSM), X-ray powder diffraction (XRD), and X-ray photoelectron spectroscopy (XPS), respectively. The FeO-MNRs were used as Fenton-like catalysts in the presence of hydrogen peroxide (HO) and showed excellent Fenton-catalytic activity for degradation of organic dyes such as Methylene blue (MB), Rhodamine B (RhB), and Bromophenol blue (BPB).