Publications by authors named "Xianyi Su"
Article Synopsis
- - The study presents a new method for separating extracellular vesicles (EVs) from plasma, focusing on their surface charges to improve the purity of EVs and reduce contamination by free plasma proteins.
- - This charge-based fractionation technique was optimized through various analyses, including proteomics and electron microscopy, ensuring the effective isolation of EVs from healthy donors.
- - The method was tested on clinical prostate cancer samples, showing promise for enhancing EV-based diagnostics and research, while also being user-friendly and accessible without specialized equipment.
Extracellular vesicles (EVs) play a pivotal role in various biological pathways, such as immune responses and the progression of diseases, including cancer. However, it is challenging to isolate EVs at high purity from blood plasma and other biofluids due to their low abundance compared to more predominant biomolecular species such as lipoprotein particles and free protein complexes. Ultracentrifugation-based EV isolation, the current gold standard technique, cannot overcome this challenge due to the similar biophysical characteristics of such species.
View Article and Find Full Text PDFExtracellular vesicles (EVs) are phospholipid bilayer membrane-enclosed structures containing RNAs, proteins, lipids, metabolites, and other molecules, secreted by various cells into physiological fluids. EV-mediated transfer of biomolecules is a critical component of a variety of physiological and pathological processes. Potential applications of EVs in novel diagnostic and therapeutic strategies have brought increasing attention.
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