More than sleep problems? Testing five key health behaviors as reasons for quality of life issues among shift workers.

Background: The shift work schedule is a common work arrangement that can disrupt typical sleep-wake rhythms and lead to negative health consequences. The present study aims to examine the effect of shift work on health-related quality of life (QoL) and explore potential behaviorial mediators (i.e.

Clinical, epidemiological, and drug resistance insights into HIV-positive patients in Meizhou, China.

The acquired immunodeficiency syndrome (AIDS) epidemic, resulting from human immunodeficiency virus (HIV) infection, exhibits distinct regional characteristics. This study undertakes a retrospective analysis of the epidemiological and clinical features of 195 HIV-positive cases in Meizhou, China, from May 1, 2018 to December 31, 2019. Western blotting (WB) confirmed and assessed these cases.

Three new LmbU targets outside lmb cluster inhibit lincomycin biosynthesis in Streptomyces lincolnensis.

Background: Antibiotics biosynthesis is usually regulated by the cluster-situated regulatory gene(s) (CSRG(s)), which directly regulate the genes within the corresponding biosynthetic gene cluster (BGC). Previously, we have demonstrated that LmbU functions as a cluster-situated regulator (CSR) of lincomycin. And it has been found that LmbU regulates twenty non-lmb genes through comparative transcriptomic analysis.

Association of Apolipoprotein E Gene Polymorphism with Type 2 Diabetic Nephropathy in the Southern Chinese Population.

Background: Common polymorphisms within the apolipoprotein E (APOE) gene are rs429358 and rs7412, which result in three major alleles (ɛ2, ɛ3, and ɛ4) and six genotypes (E2/E2, E2/E3, E3/E3, E3/E4, E4/E4, and E2/E4). Although gene polymorphisms have been suggested to be associated with the development of diabetic nephropathy (DN), their potential association remains unclear in different regions. This study aims to unveil the genetic effects of gene polymorphisms on DN susceptibility and serum lipid profiles in southern Chinese population.

Spatial Pattern Characteristics and Factors for the Present Status of Rural Settlements in the Lijiang River Basin Based on ArcGIS.

In China, rural settlements have undergone significant changes in response to dramatic socioeconomic shifts. However, there has not been any report on rural settlements in the Lijiang River Basin. In this study, ArcGIS 10.

Exploring the effect of use contexts on user engagement toward tourism short video platforms.

With tourism short video platforms' increasingly fierce competition, retaining existing users and improving engagement has taken on greater theoretical and practical significance. Based on the self-system model of motivational development, this study, involving an empirical analysis of 252 user data samples, establishes a research model to determine how the use contexts affect users' psychological process and finally lead to behavioral engagement. In particular, four use contexts of tourism short video platforms are proposed-namely, information acquisition, leisure and entertainment, attention obtainment, and social interaction.

Trends in Incidence and Mortality of Stroke in China From 1990 to 2019.

Stroke is a leading cause of mortality and morbidity globally. This study aimed to analyze the burden and 30-year trends of ischemic stroke, intracerebral hemorrhage (ICH), and subarachnoid hemorrhage (SAH) in China. Data that include incidence and mortality of stroke in China from January 1, 1990 to December 31, 2019 were derived from the Global Burden of Disease (GBD) study 2019.

Heterologous expression and metabolic engineering tools for improving terpenoids production.

Terpenoids, also referred to as isoprenoids, are the largest group of natural compounds which have contributed significantly to the pharmaceutical industry. The challenges in producing bioactive terpenoids from their original host or by organic synthesis methods spurred the endeavors of producing terpenoids in heterologous host. Modern advances utilizing synthetic biology and biological engineering tools have provided a variety of pharmaceutical terpenoids in large-scale and with diversified structures.

Overexpression of Global Regulator PbrlaeA Leads to the Discovery of New Polyketide in Fungus HDN-12-143.

Overexpression of the PbrlaeA gene of the fungus HDN-12-143 resulted in the isolation of four compounds including fumigatin chlorohydrin (), whose configuration has not been reported before, and one new compound iso-fumigatin chlorohydrin (). All structures including absolute configurations were elucidated on the basis of comprehensive spectroscopic data, C NMR calculations, and ECD calculations. Compounds and exhibited cytotoxic activity against HL-60 with IC of 18.

Identification of and solution for false D-dimer results.

Background: Clinically, D-dimer (DD) levels are mainly used to exclude diseases such as deep venous thrombosis (DVT). In clinical testing, DD assays can be subjected to interference that may cause false results, which directly affect the clinical diagnosis. Our hypothesis was that the 95% confidence intervals (CIs) of the fibrin degradation product (FDP)/DD and fibrinogen (Fib)/DD ratios were used to identify these false results and corrected via multiple dilutions.

Discovery of Two New Sorbicillinoids by Overexpression of the Global Regulator LaeA in a Marine-Derived Fungus YJ-11.

Overexpression of the global regulator LaeA in a marine-derived fungal strain of YJ-11 induced obvious morphological changes and metabolic variations. Further chemical investigation of the mutant strain afforded a series of sorbicillinoids including two new ones named 10,11-dihydrobislongiquinolide () and 10,11,16,17-tetrahydrobislongiquinolide (), as well as four known analogues, bislongiquinolide (), 16,17-dihydrobislongiquinolide (), sohirnone A (), and 2',3'-dihydrosorbicillin (). The results support that the global regulator LaeA is a useful tool in activating silent gene clusters in strains to obtain previously undiscovered compounds.

What makes D-dimer assays suspicious-heterophilic antibodies?

Background: Heterophilic antibodies are still an important source of interference in immunoassays, but reports of interference with D-dimers are rare. Are D-dimer level abnormalities, found in the clinic, caused by heterophilic antibodies as well, or are other mechanisms involved? We will elaborate on this issue through two different examples in this article.

Methods: Serum from two patients with significantly elevated levels of D-dimers were measured and compared by different methods, diluted, and dealt with heterophilic antibody blockers.

[Quantitative Analysis of Mn in Soil Samples Using LIBS].

The trace element of Manganese element in the agricultural farm (Anhui Huaiyuan Nongkang) soil was quantitatively analyzed by Laser-induced breakdown spectroscopy. The line of 403.1 nm was selected as the analysis line of Mn.