Non-linear relationship between the first meal time of the day and gallstone incidence in American adults: a population-based cross-sectional study.

Background: Irregular meal time is associated with gallstones. The time-dose effect between meal time and gallstone formation remains unknown.

Objective: This study aimed to investigate the association between the first meal time (FMT) of the day and the prevalence of gallstones.

A Novel HLA-C Allele, HLA-C*12:419, Was Identified by Next-Generation Sequencing in a Chinese Individual.

HLA-C*12:419 has a single nucleotide substitution at position 445 G>T when compared to the C*12:02:02:25 allele.

Development of a multiplex real-time PCR for the simultaneous detection of monkeypox virus clades I, II, and goatpox virus.

Introduction: Monkeypox virus (MPXV) hosts are of multiple species, with a risk of cross-species transmission. This phenomenon poses a threat to unreported affected domestic animals and increases the risk to human public health. Clinical diagnostics continue to face challenges regarding specificity among poxviruses.

Quantitative analysis of dried serum FTIR spectra based on correlation Analysis-Interval random Frog-Partial least squares.

Serum biochemical markers are widely used in clinical practice but often require expensive, specific reagents, complex instruments, and prolonged result waiting times. Infrared spectroscopy offers multiple advantages for serum analysis, such as reagent-free testing and the ability to quickly and directly measure multiple parameters simultaneously. This study collected serum samples from 66 healthy subjects to explore the relationship between dried serum infrared spectra and biochemical parameters, and to investigate the feasibility of simultaneously quantifying nine major serum components using dried serum infrared spectra.

Enhanced carbonate weathering and CO release in a typical karst watershed (Southwest China): Evidence from hydrochemical and multi-isotopic data.

Carbon sinks formed by carbonate weathering are a major component of the carbon sink deficit and play a significant role in the carbon cycle. However, exogenous acids (HNO and HSO) are also involved in carbonate weathering, complicating the overestimation of the formation of carbon sinks and fluxes. In this study, we analyzed the Hongjiadu karst underground river basin in Guizhou using isotopes (δC-DIC, δS-SO, δO-SO, δO-HO, and δH-HO) and hydrochemical methods to investigate carbon cycling characteristics and its influencing factors, and quantitatively evaluate the effects of HNO + HSO on CO sink fluxes in the basin.

A splice mutation in gene in the patient with recurrent epistaxis and nasal vascular malformation.

Platelet type bleeding disorder-18 (BDPLT18) caused by mutations of Ras guanyl releasing protein 2 () is a relatively rare, new autosomal recessive disorder. Here, we reported a splice mutation in gene in the patient with recurrent epistaxis and nasal vascular malformation. The patient, an 8-year-old girl, suffered from anemia due to frequently severe recurrent epistaxis, requiring regular blood transfusions every 2-3 months.

A Novel HLA-DPA1 Allele, HLA-DPA1*01:204, Was Identified by Next-Generation Sequencing.

HLA-DPA1*01:204 has a single nucleotide substitution at position 426G > T when compared to the HLA-DPA1*01:03:01:15 allele.

Therapeutic effects of Kangxian Yanshen formula on patients with chronic kidney disease stages 3-4: a retrospective cohort study.

Background: This study retrospectively evaluated the actual efficacy of Kangxian Yanshen Formula Chinese medicine on renal function-related indicators in chronic kidney disease (CKD) stage 3-4 patients.

Methods: In this retrospective cohort study, we collected 212 adult CKD patients with baseline estimated glomerular filtration rate (eGFR) of 15-60 ml/min/1.73 m.

pH-sensitive and redox-responsive poly(tetraethylene glycol) nanoparticle-based platform for cancer treatment.

Effective drug delivery with precise tumour targeting is crucial for cancer treatment. To address the challenges posed by the specificity and complexity of the tumour microenvironment, we developed a poly(tetraethylene glycol)-based disulfide nanoparticle (NP) platform and explored its potential in cancer treatment, focusing on drug loading and controlled release performance. Poly(tetraethylene glycol) NPs were characterised using nuclear magnetic resonance spectroscopy, mass spectrometry, and ultraviolet-visible spectroscopy.

Development of a double-antibody sandwich ELISA for quantification of mutated EPSPS gene expression in rice.

Glyphosate resistance is a critically important trait for genetically modified (GM) crops. Mutation of the rice EPSPS gene results in a high level of glyphosate resistance, presenting significant potential for the development of glyphosate-tolerant crops. The resistance of rice to glyphosate is correlated with the expression levels of resistance genes.

Insight into the transcriptional regulation of key genes involved in proline metabolism in plants under osmotic stress.

Proline biosynthesis and catabolism pathways are executed by powerful action of specific enzymes that are subjected to environmental fluctuations at the transcriptional level. Previous researches have demonstrated that osmotic stress-induced upstream events can affect the expression of proline metabolism-related genes, which results in adjustable free proline accumulation to protect plant cells from severe damage. Here, we mainly describe the mechanisms for how some key factors, such as transcription factors, ABA (abscisic acid), Ca, MAPK cascades, CK (cytokinin) and phospholipase, in a phosphorylated manner, vividly function in the transcriptional regulation of proline metabolism under osmotic stress.

Challenges and innovations in CAR-T cell therapy: a comprehensive analysis.

Recent years have seen a marked increase in research on chimeric antigen receptor T (CAR-T) cells, with specific relevance to the treatment of hematological malignancies. Here, the structural principles, iterative processes, and target selection of CAR-T cells for therapeutic applications are described in detail, as well as the challenges faced in the treatment of solid tumors and hematological malignancies. These challenges include insufficient infiltration of cells, off-target effects, cytokine release syndrome, and tumor lysis syndrome.

The long-term efficacy of eltrombopag in children with immune thrombocytopenia.

Immune thrombocytopenia (ITP) is the most common autoimmune disorder characterized by decreased platelet counts and impaired platelet production. Eltrombopag has been demonstrated to be safe and effective for children with ITP. It is reported eltrombopag can achieve a sustained response off treatment.

A Coupled System of NiS and Rh Complex with Biomimetic Function for Electrocatalytic 1,4-NAD(P)H Regeneration.

NAD(P)H cofactor is a critical energy and electron carrier in biocatalysis and photosynthesis, but the artificial reduction of NAD(P) to regenerate bioactive 1,4-NAD(P)H with both high activity and selectivity is challenging. Herein, we found that a coupled system of a NiS electrode and a Rh complex in an electrolyte (denoted as NiS-) can catalyze the reduction of NAD(P) to 1,4-NAD(P)H with superior activity and selectivity. The optimized selectivity in 1,4-NADH can be up to 99.

Identification of α-Glucosidase-Inhibitors in Edgeworthia gardneri (Wall.) Meisn. Using UPLC-Q-TOF-MS/MS Analysis.

Edgeworthia gardneri (Wall.) Meisn., a member of the genus Edgeworthia in the family Thymelaeaceae, has long been applied as an edible and medicinal plant in China.

Thioredoxin-1 decreases alpha-synuclein induced by MPTP through promoting autophagy-lysosome pathway.

Parkinson's disease (PD) is characterized by the formation of Lewy body in dopaminergic neurons in the substantia nigra pars compacta (SNpc). Alpha-synuclein (α-syn) is a major component of Lewy body. Autophagy eliminates damaged organelles and abnormal aggregated proteins.

Effects of sodium-glucose cotransporter-2 (SGLT-2) inhibitors on serum uric acid levels in patients with chronic kidney disease: a systematic review and network meta-analysis.

Elevated serum uric acid levels are an independent predictor of occurrence and development of chronic kidney disease (CKD) and are strongly associated with prognosis. Several clinical trials have demonstrated the benefits of sodium-glucose cotransporter-2 (SGLT-2) inhibitors. To evaluate and rank the effects and safety of various SGLT-2 for serum uric acid levels in patients with CKD.