Publications by authors named "Xianjun Chen"

RNA performs a remarkable range of functions, such as RNA processing, chromosome maintenance and dosage compensation. Technologies that robustly and specifically image RNA in its native state are highly desirable, as these technologies can help researchers clarify the localization and functionality of diverse RNAs. Here, we describe the development of a sequence-activated fluorescent RNA (SaFR) technique.

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Fluorescent RNAs (FRs), which are RNA aptamers that bind and activate their cognate small fluorogenic dyes, have provided a particularly useful approach for imaging RNAs in live cells. Although the color palette of FRs is greatly expanded, a bright and stable cyan FR with good biocompatibility and biorthogonality with currently available FRs remains desirable but is not yet developed. Herein, the development of Myosotis is described, an RNA aptamer that emits bright cyan fluorescence upon binding a novel GFP chromophore-like fluorophore called DBT.

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  • * The researchers developed high-performance fluorescent biosensors called sensitive to arginine (STAR) that allow for real-time monitoring of arginine levels in cells, mice, and clinical samples.
  • * Using STAR, they discovered the influence of different amino acids on arginine levels and found that serum arginine levels tend to rise with age, which could help in assessing the progression of conditions like vitiligo.
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  • Telomeres are protective DNA sequences at the ends of chromosomes that shorten during cell division, requiring the shelterin protein complex to maintain their stability and prevent genomic issues.
  • TERRA, a noncoding RNA derived from telomeres, is crucial for regulating telomere stability, and the hnRNPs, particularly hnRNPA1, interact with both TERRA and telomeric DNA, playing a role in telomere maintenance.
  • The study identifies how the UP1 domain of hnRNPA1 interacts with TERRA, revealing key residues involved and showing that this interaction is essential for hnRNPA1's function and droplet formation, which is important for maintaining genomic stability.
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  • Magnesium-based scaffolds are becoming popular for bone repair due to their biodegradability and similarity to natural bone, but their clinical use is limited by weak bonding and unclear coating mechanisms.
  • This study introduces a new composite coating called polydopamine-microarc oxidation (PDA-MHA), which significantly increases bonding strength and enhances hydrophilicity while controlling degradation rates of the scaffolds.
  • The PDA-MHA-coated scaffolds promote bone regeneration by influencing key osteogenic markers and activating pathways that support bone healing, suggesting their potential in clinical applications for bone tissue engineering.
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Background: Osteomyelitis (OM) is recognized as a significant challenge in orthopedics due to its complex immune and inflammatory responses. The prognosis heavily depends on timely diagnosis, accurate classification, and assessment of severity. Thus, the identification of diagnostic and classification-related genes from an immunological standpoint is crucial for the early detection and tailored treatment of OM.

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Reduced glutathione (γ-glutamyl-cysteinyl-glycine, GSH), the primary non-protein sulfhydryl group in organisms, plays a pivotal role in the plant salt stress response. This study aimed to explore the impact of GSH on the photosynthetic apparatus, and carbon assimilation in tomato plants under salt stress, and then investigate the role of nitric oxide (NO) in this process. The investigation involved foliar application of 5 mM GSH, 0.

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In this study, processing tomato ( L.) 'Ligeer 87-5' was hydroponically cultivated under 100 mM NaCl to simulate salt stress. To investigate the impacts on ion homeostasis, osmotic regulation, and redox status in tomato seedlings, different endogenous levels of ascorbic acid (AsA) were established through the foliar application of 0.

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  • - RNA-based fluorogenic modules, like the newly identified red-emitting fluorophore NBSI and its aptamer Clivia, have advanced our ability to localize RNA molecules in living cells using unique fluorescence properties.
  • - Researchers determined the Clivia-NBSI structure, which features a compact arrangement with the fluorophore at its center, enabling the potential for dual-emission in imaging applications.
  • - A new multivalent Clivia aptamer was created, enhancing fluorophore recognition sensitivity significantly, which could lead to improved techniques in biomedical research and other applications.
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  • - Researchers developed a new fluorogenic RNA aptamer called Okra that significantly enhances the visibility of RNA in live cells by binding to a ligand and producing bright green fluorescence.
  • - Okra offers improved cellular brightness compared to existing green fluorescent RNAs, making it effective for imaging messenger RNA in both bacterial and mammalian cells.
  • - The study demonstrates Okra's potential for advanced imaging techniques, such as tracking ACTB mRNA in stress granules and dual-color superresolution imaging, highlighting its capability for studying RNA dynamics and localization.
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Introduction: In rare occasions, coxsackievirus infections can cause serious illness, such as encephalitis and myocarditis. The immunotherapies of cancer could increase the risk of myocarditis, especially when applying immune checkpoint inhibitors. Herein, we report a rare case of Coxsackie B virus-induced myocarditis in a patient with a history of lymphoma.

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Purpose: The purpose of this study was to investigate the risk factors for umbilical artery thrombosis (UAT) and the relationship between umbilical artery thrombosis and perinatal outcomes.

Methods: This was a retrospective study that enrolled singleton pregnant women who were diagnosed with umbilical artery thrombosis. The control group recruited pregnant woman with three umbilical vessels or those with isolated single umbilical artery (iSUA) who were matched with umbilical artery thrombosis group.

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Fluorescent RNA is a kind of emerging RNA labeling technique that can be used for labeling and imaging of RNA in live cells, which plays an important role in understanding the function and regulation mechanism of RNA. Biosensing technology based on fluorescent RNA can be applied in dynamic detection of small molecule metabolites and proteins in real time, offering valuable tools for basic life science research and biomedical sensing technology development. In this review, we introduce the development of genetically encoded fluorescent RNA, and the application of fluorescent RNA in RNA imaging and biosensing technology based on fluorescent RNA in biosensing in live cell.

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Background: Alveolar hypercoagulation and fibrinolytic inhibition are mainly responsible for massive alveolar fibrin deposition, which are closely related with refractory hypoxemia in acute respiratory distress syndrome (ARDS). Our previous study testified runt-related transcription factor (RUNX1) participated in the regulation of this pathophysiology in this syndrome, but the mechanism is unknown. We speculate that screening the downstream genes associated with RUNX1 will presumably help uncover the mechanism of RUNX1.

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Background: Alveolar hypercoagulation and fibrinolytic inhibition play a central role in refractory hypoxemia in acute respiratory distress syndrome (ARDS), but it lacks effective drugs for prevention and treatment of this pathophysiology. Our previous experiment confirmed that RUNX1 promoted alveolar hypercoagulation and fibrinolytic inhibition through NF-κB pathway. Other studies demonstrated that 6-gingerol regulated inflammation and metabolism by inhibiting the NF-κB signaling pathway.

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  • Self-healing coatings are being developed to prevent degradation and detachment issues in magnesium (Mg) scaffolds used in medical applications, especially in infection-prone areas.* -
  • The researchers created a specific self-healing coating, named OD-MHA/Mg, using oxidized dextran, APTES, and nano-hydroxyapatite, which effectively prevents coating issues and controls Mg degradation.* -
  • This innovative coating not only offers antibacterial and antioxidant properties but also supports bone repair by enhancing gene expression related to osteogenesis, making it a promising solution for treating infectious bone defects.*
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  • The objective of the study was to investigate the diagnosis and treatment of acute cerebral infarction in patients experiencing cardiogenic shock after receiving extracorporeal membrane oxygenation (ECMO) therapy.
  • Two cases were analyzed from patients treated in an ICU, providing insights into their clinical progression and complications related to ECMO.
  • In Case 1, a 46-year-old male experienced severe heart issues leading to acute cerebral infarction and ultimately died after ECMO support; while Case 2 involved a 43-year-old male with cardiac complications, indicating the serious risks associated with ECMO in such patients.
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RNA molecules perform various crucial roles in diverse cellular processes, from translating genetic information to decoding the genome, regulating gene expression and catalyzing chemical reactions. RNA-binding proteins (RBPs) play an essential role in regulating the diverse behaviors and functions of RNA in live cells, but techniques for the spatiotemporal control of RBP activities and RNA functions are rarely reported yet highly desirable. We recently reported the development of LicV, a synthetic photoswitchable RBP that can bind to a specific RNA sequence in response to blue light irradiation.

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The increased risk of breast cancer metastasis is closely linked to the effects of platelets. Our previously light-switchable diphtheria toxin A fragment (DTA) gene system, known as the LightOn system, has demonstrated significant therapeutic potential; it lacks antimetastatic capabilities. In this study, we devised an innovative system by combining cell membrane fusion liposomes (CML) loaded with the light-switchable transgene DTA (pDTA) and a ticagrelor (Tig) prodrug.

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There are still many unresolved problems in the treatment and prognosis of nondisplaced femoral neck fractures, such as nonunion and avascular necrosis of the caput femoris .In order to reduce the risk of various complications after non-displaced femoral neck fractures, the caput femoris posterior tilt of femoral neck fractures and its impact on prognosis have attracted more and more attention. A large number of scholars' studies have found that when the posterior tilt exceeds 20°, the risk of internal fixation failure increases significantly.

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  • Fluorescent RNAs, particularly Clivias, are innovative tools for visualizing RNA in live cells, overcoming challenges related to imaging multiple RNAs simultaneously.
  • Clivias are small, stable, and have large Stokes shifts, allowing for effective RNA imaging with minimal disruption to RNA function.
  • They enable advanced techniques, like dual-color imaging and RNA-protein interaction detection, which are valuable for studying various biological processes.
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Objective: The present study was designed to explore the association between serum sodium and mortality in patients with sepsis by using a large sample, multicenter MIMIC-IV database.

Methods: We extracted the data of 34 925 sepsis patients from the retrospective cohort mimicIV database. After adjusting the confounders, we explored the independent effects of serum sodium on 28-day mortality.

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Objective: Alveolar type II (ATII) cells produce pulmonary surfactant (PS) essential for maintaining lung function. The aberration or depletion of PS can cause alveolar collapse, a hallmark of acute respiratory distress syndrome (ARDS). However, the intricacies underlying these changes remain unclear.

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The tumor microenvironment is a barrier to breast cancer therapy. Cancer-associated fibroblast cells (CAFs) can support tumor proliferation, metastasis, and drug resistance by secreting various cytokines and growth factors. Abnormal angiogenesis provides sufficient nutrients for tumor proliferation.

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