Prevention of supercritical carbon dioxide fluid extract from Linnén on cutaneous squamous cell carcinomas progression following UV irradiation in mice.

Linnén (), a medicinal and food herb with various bioactive components, may be of beneficial use in cosmetics and the treatment of skin-related diseases. However, to date, few studies have been reported on its potential preventive and therapeutic effects on skin cancer. Therefore, the present study aimed to investigate the effect and potential mechanism of action of supercritical carbon dioxide extract from (CI) on UV-induced skin cancer in a mouse model.

Asperuloside suppressing oxidative stress and inflammation in DSS-induced chronic colitis and RAW 264.7 macrophages via Nrf2/HO-1 and NF-κB pathways.

Background: Inflammatory bowel diseases (IBDs), which mainly include Crohn's disease (CD) and ulcerative colitis (UC), are chronic idiopathic inflammatory disease of the gastrointestinal tract for which effective pharmacological treatments are lacking or options are very limited.

Purpose: Here, we aim to investigate the therapeutic effects of an iridoid glycoside, asperuloside (ASP) on mice experimental chronic colitis induced by dextran sulfate sodium (DSS) and further explore underlying mechanisms in vitro and in vivo.

Methods: LPS-treated RAW 264.

Chemical Constituents from Sargassum fusiforme (Harv.) Setch.

Phytochemical investigation of Sargassum fusiforme (Harv.) Setch. led to the discovery of fifteen secondary metabolites, including three sterols, three monoterpenes, five nitrogenous compounds, two fatty acids, and two others.

Vitexin protects against ethanol-induced liver injury through Sirt1/p53 signaling pathway.

In the present study, we aimed to investigate the therapeutic effect of Vitexin on inhibiting ethanol-induced liver damage and explore the underling mechanism. In vitro, the injury was induced in LO2 cell by 100 mM ethanol. Cell viability, AST, oxidative stress, inflammation, apoptosis rate, and related gene and protein expressions were assessed.

Effect of vitexin on alleviating liver inflammation in a dextran sulfate sodium (DSS)-induced colitis model.

As one of commonly used herbs with dual-purpose of drug and food, it has been reported that vitexin has hepatoprotective effects. However, the protective effects of vitexin on colitis-induced liver injury as well as the underlying molecular mechanisms remain unclear. The purpose of the current study was to investigate the effects and mechanisms of vitexin on liver injury induced by acute ulcerative colitis in mice.

Dendrobium officinale polysaccharides alleviate colon tumorigenesis via restoring intestinal barrier function and enhancing anti-tumor immune response.

Intact epithelial barrier and mucosal immune system are crucial for maintaining intestinal homeostasis. Previous study indicated that Dendrobium officinale polysaccharides (DOPS) can regulate immune responses and inflammation to alleviate experimental colitis. However, it remains largely unknown whether DOPS can suppress AOM/DSS-induced colorectal cancer (CRC) model through its direct impact on intestinal barrier function and intestinal mucosal immunity.

[Inhibition of sanguinarine on S180 subcutaneously implanted tumors in mice].

Objective: To investigate the inhibition of sanguinarine on S180 sarcoma in mice and the effect of angiogenesis.

Methods: S180 subcutaneous implanted tumor model mice were randomly divided into six groups: control group, cyclophosphamide (CTX) group, sanguinarine (10, 20 and 40 mg/kg) groups and combination group. The mice were sacrificed on the 10th day to measure the tumor weight and volumes, and caculate the tumor growth inhibition.

[Study on absorption mechanism of genistein self-microemulsifying system in rat intestines].

Objective: To investigate the absorption mechanism of genistein self-microemulsifying system in rat intestines.

Method: The concentrations of phenol red and genistein by in situ perfusion in rats were determined by UV and HPLC, respectively. The effects of drug concentrations, pH, various intestinal segments and P-glycoprotein (P-gp) inhibitor verapamil on the absorption had been studied.

[Effects of Salviae miltiorrhizae on H2O2-induced apoptosis of rat vascular smooth muscle cells].

Objective: To investigate the effects of Salviae miltiorrhizae on H2O2-induced apoptosis in rat vascular smooth muscle cells (VSMC(s)) and its related mechanism.

Methods: The 3-5 passages of VSMC(s) were used, H2O2 was added to the medium of cultured rat aortic VSMC(s) as extraneous reactive oxygen species (ROS). The viability percentage of VSMC(s) was evaluated by MTT colorimetric assay.

[The mechanism of Radix Salviae Miltiorrhizae Injection on the inhibition of smooth muscle cell proliferation induced by H2O2].

Objective: To observe the effect of Radix Salviae Miltiorrhizae Injection (RSMI) on proliferating cell nuclear angiten (PCNA), NF-kappa B and cell cycle phase of vascular smooth muscle cell(VSMC) induced by H2O2 in vitro.

Methods: H2O2 was added to the medium for culturing rat aortic VSMC as extraneous reactive oxygen species (ROS). The protein expression of PCNA and NF-kappa B was examined with immunocytochemical technique in VSMC, and cell cycle phase analysis was used to detect proliferation of VSMC.