Directed differentiation of human embryonic stem cells into conjunctival epithelial cells.

Severe conjunctival damage can lead to extensive ocular cicatrisation, fornix shortening, and even ocular surface failure, resulting in significant vision impairment. Conjunctival reconstruction is the primary therapeutic strategy for these clinical conjunctival diseases. However, there have been limited studies on induced differentiation of conjunctival epithelial cells derived from stem cells.

Acoustic vibration promotes in vitro expansion of human embryonic stem cells.

Objectives: This study aimed to investigate the effect of acoustic vibration on the pluripotency of human embryonic stem cells (hESCs) and evaluate cell proliferation and self-renewal ability post-treatment.

Methods: The human ES cell line H1 was used for the experiments. hESCs were treated with an acoustic vibration device.

Nicotinamide promotes the differentiation of functional corneal endothelial cells from human embryonic stem cells.

Corneal transplantation represents the primary therapeutic approach for managing corneal endothelial dysfunction, but corneal donors remain scarce. Anterior chamber cell injection emerges as a highly promising alternative strategy for corneal transplantation, with pluripotent stem cells (PSC) demonstrating considerable potential as an optimal cell source. Nevertheless, only a few studies have explored the differentiation of functional corneal endothelial-like cells originating from PSC.

[Geostatistical analysis on temporal patterns of Chilo suppressalis population].

The geostatistical analysis on the temporal patterns of Chilo suppressalis population in the Dingcheng District of Changde City, Hunan Province from 1960 to 2001 indicated that the data series of the total number and the numbers of 1st generation, 2nd generation, and over-wintering larvae from year to year displayed better autocorrelation and prediction, especially for the total number, whose autocorrelation range and degree were 10.3 years and 91.1%, respectively.

[Krigle estimation and its simulated sampling of Chilo suppressalis population density].

In order to draw up a rational sampling plan for the larvae population of Chilo suppressalis, an original population and its two derivative populations, random population and sequence population, were sampled and compared with random sampling, gap-range-random sampling, and a new systematic sampling integrated Krigle interpolation and random original position. As for the original population whose distribution was up to aggregative and dependence range in line direction was 115 cm (6.9 units), gap-range-random sampling in line direction was more precise than random sampling.