Publications by authors named "Xiangye Zhang"

Macrophages make up a heterogeneous population of immune cells that exhibit diverse phenotypes and functions in health and disease. Although macrophage epigenomic and transcriptomic profiles have been reported, the proteomes of distinct macrophage populations under various pathological conditions remain largely elusive. Here, we employed a label-free proteomic approach to characterize the diversity of the hepatic macrophage pool in an experimental model of CCl-induced liver fibrosis.

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Organophosphorus compounds (OPs) such as chemical agents and pesticides are posing critical threats to civilians due to their irreversible phosphonylation of diverse amino acids residues forming different protein adducts. However, traditional analytical approaches are quite limited in capturing the myriad of post-translational events that affect protein functions, especially in identifying the low-abundance OP adducts. Herein a systematic proteomic strategy based on a typical click-enrich-release-identify bioorthogonal operation was firstly developed by employing an alkynyl-tagged V-type agent probe (AVP) and a biotin-based azido-enrichment linker (BTP-N ).

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Post-translational modifications (PTMs), such as phosphorylation and ubiquitination, play key roles in signal transduction and protein homeostasis. The crosstalk of PTMs greatly expands the components of proteome and protein functions. Multi-level proteome analysis, which involves proteome investigations of total lysate and PTMs in this context, provides a comprehensive approach to explore the PTM crosstalk of a biological system under diverse disturbances.

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Article Synopsis
  • Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are serious complications of COVID-19, and clofazimine (CFZ) has potential due to its anti-inflammatory effects.
  • A study employed quantitative proteomics to analyze the effects of CFZ on macrophages exposed to LPS-induced inflammation, identifying significant proteins and pathways related to inflammation, such as TLR4/NF-κB/HIF-1α.
  • The findings indicated that CFZ reduced inflammatory markers, improved lung health, and increased survival rates, suggesting its potential use as a therapeutic strategy for treating COVID-19 related inflammation.
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