On-site detection of Sporothrix globosa using immunoglobulin Y, magnetic separation and loop-mediated isothermal amplification.

Background: Sporothrix globosa (S. globosa) is an agricultural activity-related but neglected pathogenic fungus responsible for sporotrichosis. Timely detection is crucial for managing and preventing its spread.

Rapid qualitative and quantitative detection of Salmonella typhimurium using a single-step dual photometric/fluorometric assay.

A dual-signal photometric/fluorometric assay was established for rapid, qualitative, and quantitative detection of Salmonella typhimurium (S. typhimurium). This method was composed of two parts: (1) a single-step photometric (SSC) assay containing gold nanoparticles (AuNPs), poly-diallyldimethylammonium chloride (PDDA), and S.