Pyrite (FeS) has garnered attention due to its narrow bandgap, high light absorption, and low cost. However, the rapid recombination of charge carriers hinders its practical application. Surface electric field is a unique characteristic of tourmaline, which can induce effective separation of photo generated electrons and holes.
View Article and Find Full Text PDFNormal testicular development ensures the process of spermatogenesis, which is a complex biological process. The sustained high productivity of spermatogenesis throughout life is predominantly attributable to the constant proliferation and differentiation of spermatogonial stem cells (SSCs). The self-renewal and differentiation processes of SSCs are strictly regulated by the SSC niche.
View Article and Find Full Text PDFHematite (FeO) has garnered attention due to its stability, economic viability, and non-toxic nature. However, the rapid recombination of charge carriers hampers its practical application. On the other hand, tourmaline's inherent surface electric field facilitates the rapid separation of photogenerated electrons and holes.
View Article and Find Full Text PDFparticipates in methylation, yet its impact on the proliferation of testicular Sertoli cells remains unclear. Development-specific methylation has been proven to be associated with cellular development. Therefore, in this study, we simulated expression pattern during testicular development by interference.
View Article and Find Full Text PDFRNA -methyladenosine (mA) modification is one of the principal post-transcriptional modifications and plays a dynamic role in testicular development and spermatogenesis. However, the role of mA in porcine testis is understudied. Here, we performed a comprehensive analysis of the mA transcriptome-wide profile in Shaziling pig testes at birth, puberty, and maturity.
View Article and Find Full Text PDFIn the genomes of diploid organisms, runs of homozygosity (ROH), consecutive segments of homozygosity, are extended. ROH can be applied to evaluate the inbreeding situation of individuals without pedigree data and to detect selective signatures via ROH islands. We sequenced and analyzed data derived from the whole-genome sequencing of 97 horses, investigated the distribution of genome-wide ROH patterns, and calculated ROH-based inbreeding coefficients for 16 representative horse varieties from around the world.
View Article and Find Full Text PDFIn the field of photocatalysis, Graphitic carbon nitride (g-CN) has received a lot of attention for its superior functionality and benefits. However, it suffers from the fatal defect of low charge separation efficiency, which is well addressed by tourmaline's self-contained surface electric field. In this work, tourmaline/g-CN (T/CN) composites were successfully synthesized.
View Article and Find Full Text PDFUnderstanding the genetic variations of the horse (Equus caballus) genome will improve breeding conservation and welfare. However, genetic variations in long segments, such as structural variants (SVs), remain understudied. We de novo assembled 10 chromosome-level three-dimensional horse genomes, each representing a distinct breed, and analysed horse SVs using a multi-assembly approach.
View Article and Find Full Text PDFThe regulatory role of non-CpG methylation in mammals has been important in whole-genome bisulfite sequencing. It has also been suggested that non-CpG methylation regulates gene expression to affect the development and health of mammals. However, the dynamic regulatory mechanisms of genome-wide, non-CpG methylation during testicular development still require intensive study.
View Article and Find Full Text PDFAlbendazole is a broad-spectrum fungicide that shows great potential in controlling fungal diseases in citrus. To quantify the dissipation behavior, residue distribution, and dietary risk of albendazole in citrus, we developed an UPLC-MS/MS analysis protocol. The average recovery rate of albendazole in whole citrus and citrus pulp ranged from 74 to 105% with an RSD of 3 to 8%, and a limit of quantification of 0.
View Article and Find Full Text PDFDNA methylation is one of the key epigenetic regulatory mechanisms in development and spermatogenesis. However, the dynamic regulatory mechanisms of genome-wide DNA methylation during testicular development remain largely unknown. Herein, we generated a single-base resolution DNA methylome and transcriptome atlas of precocious porcine testicular tissues across three developmental stages (1, 75, and 150 days old).
View Article and Find Full Text PDFPhotocatalysis is an effective, inexpensive and environmentally friendly technology for the decomposition of various aqueous organic pollutants and plays an increasingly critical role in the degradation of pollutants. Natural minerals are abundant natural resources on Earth and can be obtained directly from nature. Natural minerals are excellent photocatalyst carriers that are environmentally friendly, low in price, and will not cause secondary pollution to the environment.
View Article and Find Full Text PDFSertoli cells are the crucial coordinators to guarantee normal spermatogenesis and male fertility. Although circular RNAs (circRNAs) exhibit developmental-stage-specific expression in porcine testicular tissues and have been thought of as potential regulatory molecules in spermatogenesis, their functions and mechanisms of action remain largely unknown, especially in domestic animals. A novel circBTBD7 was identified from immature porcine Sertoli cells using reverse transcription PCR, Sanger sequencing, and fluorescence in situ hybridization assays.
View Article and Find Full Text PDFThe quantity of Sertoli cells in the adult testis decides the daily gamete formation, and accumulating evidence indicates that epigenetic factors regulate the proliferation of Sertoli cells. Research on the function and regulatory mechanism of microRNAs (miRNAs) in Sertoli cells has not been comprehensive yet, especially on domestic animals. In this article, we report that miR-126 controls the proliferation and apoptosis of immature porcine Sertoli cells based on previous studies.
View Article and Find Full Text PDFSertoli cells are central and essential coordinators of spermatogenesis. Accumulating evidence has demonstrated that miRNAs participate in the regulation of Sertoli cell growth. However, the functions and the regulatory mechanisms of miRNAs in Sertoli cells of domestic animals remain largely unknown.
View Article and Find Full Text PDFSertoli cells play vital roles in normal spermatogenesis, and microRNAs (miRNAs) participate in regulating Sertoli cell development. However, the functions and mechanisms of action of most identified miRNAs in porcine Sertoli cells remain largely unknown. Herein, we primarily explored the regulatory roles of miR-130a in immature porcine Sertoli cells using EdU-based high-content screening assay.
View Article and Find Full Text PDFImmature Sertoli cell proliferation determines the total number of mature Sertoli cells and further regulates normal spermatogenesis. Accumulating evidence demonstrates that microRNAs (miRNAs) play regulatory roles in immature Sertoli cell proliferation, while the functions and mechanisms of the Sertoli cells of domestic animals are poorly understood. In the present study, we aimed to investigate the roles of miR-362 in cell proliferation and apoptosis of porcine immature Sertoli cells.
View Article and Find Full Text PDFSertoli cells are indispensable for normal spermatogenesis, and increasing evidence has shown that miRNAs participate in the regulation of Sertoli cell growth. However, the functions and regulatory mechanisms of miRNAs in Sertoli cells of domestic animals have not been fully investigated. In the present study, we mainly investigated the regulatory roles of miR-499 in immature porcine Sertoli cells.
View Article and Find Full Text PDFSubstorms generally inject tens to hundreds of keV electrons, but intense substorm electric fields have been shown to inject MeV electrons as well. An intriguing question is whether such MeVelectron injections can populate the outer radiation belt. Here we present observations of a substorm injection of MeV electrons into the inner magnetosphere.
View Article and Find Full Text PDFDiallyl disulfide (DADS) is a volatile organosulfur compound derived from garlic (Allium sativum L.), and it is known as an allelochemical responsible for the strong allelopathic potential of garlic. The anticancer properties of DADS have been studied in experimental animals and various types of cancer cells, but to date, little is known about its mode of action as an allelochemical at the cytological level.
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