Rapid Visual Detection of High Nitrogen-Use Efficiency Gene in Rice ( L.) Using Loop-Mediated Isothermal Amplification Method.

The ( allele is an important target for the development of new high nitrogen-use efficiency (NUE) rice lines that would require less fertilizers. Detection of through PCR (polymerase chain reaction)-based assay is cumbersome and needs advanced laboratory skills and facilities. Hence, a method for conveniently and rapidly detecting on-field is a key requirement for further research and applications.