Electroenzymatic Reduction of Furfural to Furfuryl Alcohol by an Electron Mediator and Enzyme Orderly Assembled Biocathode.

The electroenzymatic valorization of biomass derivatives into valuable biochemicals has a promising outlook. However, bottlenecks including poor electron transfer between the electrode surface and oxidoreductase, inefficient regeneration of cofactors, and high cost of enzymes and electron mediators hindered the realistic applications of the technique. Herein, to address the above technical barriers, a novel bio-electrocatalytic system that integrates the electrochemical NADH regeneration and enzymatic reaction was constructed, using an orderly assembled composite bioelectrode consisting of an outer immobilized enzyme layer and a sandwiched redox mediator rhodium complex layer.

Artificial Photoenzymatic Reduction of Carbon Dioxide to Methanol by Using Electron Mediator and Co-factorAssembled ZnIn S Nanoflowers.

Increased absorption of visible light, low electron-hole recombination, and fast electron transfer are the major objectives for highly effective photocatalysts in biocatalytic artificial photosynthetic systems. In this study, a polydopamine (PDA) layer containing electron mediator, [M], and NAD cofactor was assembled on the outer surface of ZnIn S nanoflower, and the as-prepared nanoparticle, ZnIn S /PDA@poly/[M]/NAD , was used for photoenzymatic methanol production from CO . Because of effective capturing of visible light, reduced distance of electron transfer, and elimination of electron-holes recombination, a high NADH regeneration of 80.