Identification and Characterization of a Novel Prophage Lysin against .

infection can cause bovine mastitis and lead to huge economic losses for the dairy industry. The abuse of antibiotics has resulted in growing drug resistance of which causes hard-to-treat infections. Bacteriophage lysin, as a novel antibacterial agent, has great potential for application against drug-resistant gram-positive bacteria.

Simultaneously optimizing multiple properties of β-glucosidase Bgl6 using combined (semi-)rational design strategies and investigation of the underlying mechanisms.

The performance of β-glucosidase during cellulose saccharification is determined by thermostability, activity and glucose tolerance. However, conflicts between them make it challenging to simultaneously optimize three properties. In this work, such a case was reported using Bgl6-M3 as a starting point.

Spatial self-phase modulation of a Gaussian beam transmitted through a ferrofluid.

We report on the experimental observation of the diffraction pattern formed in the far-field region when a high-power continuous-wave laser convergent or divergent Gaussian beam passes through a cuvette with ferrofluid. Two different types of diffraction rings with opposite light-intensity distribution are shown in the far field. The difference between the diffractive patterns is attributed to the interaction of the strong spatial self-phase modulation caused by the refractive index change of the medium with wavefront curvature of the input Gaussian beam.

Evolution of Nonlinear Optical Characteristics of Magnetic Nanoparticle Colloidal Suspensions after Laser-Induced Clusters.

In this paper, the nonlinear optical properties of magnetic nanoparticle colloidal solutions were studied by the Z-scan technique using 25 ps laser pulses at a wavelength of 1064 nm. Our results reveal that the formed magnetic nanoparticle clusters under high incident laser intensity will greatly affect the nonlinear optical characteristics of the solution. As the intensity of the pulsed laser decreases, the reverse saturable absorption coefficient and nonlinear refractive index of the sample tend to increase.

Engineering of β-Glucosidase Bgl15 with Simultaneously Enhanced Glucose Tolerance and Thermostability To Improve Its Performance in High-Solid Cellulose Hydrolysis.

In this study, a Petri-dish-based double-layer high-throughput screening method was established to improve glucose tolerance of β-glucosidase Bgl15. Two beneficial mutations were identified, and the joint mutant 2R1 improved the half-maximal inhibitory concentration of glucose from 0.04 to 2.

Passively Q-switched Nd:YVO laser operating at 1.3 µm with a graphene oxide and ferroferric-oxide nanoparticle hybrid as a saturable absorber.

A self-made saturable absorber (SA) based on hybridized graphene oxide (GO) and ${{\rm Fe}_{3}}{{\rm O}_{4}}$FeO nanoparticles (FONP) was inserted into a linear cavity to generate a passively $ Q $Q-switched solid-state ${\rm Nd}\text:{{\rm YVO}_4}$Nd:YVO laser operating at the 1.3 µm waveband. The laser had a minimum pulse width of 163 ns and a maximum repetition rate of 314 kHz.

Improving Galactooligosaccharide Synthesis Efficiency of β-Galactosidase Bgal1-3 by Reshaping the Active Site with an Intelligent Hydrophobic Amino Acid Scanning.

There are ongoing interests in improving the galactooligosaccharide (GOS) synthesis efficiency of β-galactosidase by protein engineering. In this study, an intelligent double-hydrophobic amino acid scanning strategy was proposed and employed to target nine residues forming the glycon-binding site (-1 subsite) of β-galactosidase Bgal1-3. Two mutants C510V and H512I with significantly improved GOS synthesis efficiency were obtained.