Identification of novel biofilm genes in avian pathogenic Escherichia coli by Tn5 transposon mutant library.

Avian pathogenic Escherichia coli (APEC) is the main pathogens that inflict the poultry industry. Biofilm as the pathogenic factors of APEC, which can enhance the anti-host immune system of APEC and improve its survival in the environment. In order to screen for new genes related to APEC biofilm.

Effect of nutritional and environmental conditions on biofilm formation of avian pathogenic Escherichia coli.

Aims: To study the effects of environmental stress and nutrient conditions on biofilm formation of avian pathogenic Escherichia coli (APEC).

Methods And Results: The APEC strain DE17 was used to study biofilm formation under various conditions of environmental stress (including different temperatures, pH, metal ions, and antibiotics) and nutrient conditions (Luria-Bertani [LB] and M9 media, with the addition of different carbohydrates, if necessary). The DE17 biofilm formation ability was strongest at 25°C in LB medium.

Effect of Antibiotics on the Colonization of Live Attenuated Enteritidis Vaccine in Chickens.

Salmonellosis, caused by Enteritidis, is a prevalent zoonosis that has serious consequences for human health and the development of the poultry sector. The Enteritis live vaccine (Sm24/Rif12/Ssq strain) is used to prevent Enteritidis around the world. However, in some parts of the world, poultry flocks are frequently raised under intensive conditions, with significant amounts of antimicrobials to prevent and treat disease and to promote growth.