Publications by authors named "Xiangnan Bao"
Article Synopsis
- Mastitis (MAS), endometritis (MET), and ketosis (KET) are common diseases in dairy cows that lead to significant economic losses in the dairy industry, with prevalence rates of 20.04%, 10.68%, and 7.33%, respectively.
- These diseases negatively affect milk production and quality, causing reductions in 305-day Milk Yield, Protein Yield, and Fat Yield, with MAS and MET leading to estimated losses of up to 372 kg in milk yield per cow.
- The study also found that all three diseases increase important reproductive measures, such as the interval from calving to first service and the overall calving interval, although the herit
Gap junction channels in cumulus-oocyte complexes (COCs) enable the transmission and communication of small molecular signals between adjacent cells, such as cAMP. However, the regulation of gap junction function (GJF) by cAMP and the underlying mechanisms involved are not fully clarified. This study investigated the effect of cAMP on connexin 43 (CX43) expression and GJF in ovine COCs using immunofluorescence, quantitative real-time PCR (qRT-PCR), western blotting, and GJF detection.
View Article and Find Full Text PDFThe study was designed to investigate the effects of cryopreservation on bovine, ovine, and goat sperm motility, acrosome structure, enzyme activity, and fertilization ability. Percentage of sperm with hyaluronidase enzyme (HYD) activity was detected by a modified sodium hyaluronate-gelatin membrane. The N-α-benzoyl-DL-arginine-p-nitroanilide (BNPNA) method was used to assess the sperm acrosome enzyme (ACE).
View Article and Find Full Text PDFObjective: Owing to the lack of a breeding index for efficient and quick fertility evaluations of Holstein bulls when using traditional or genome-wide detection methods, this study aimed to determine whether in vitro fertilization (IVF) could be used as an indicator of conception rate of artificial insemination (AI).
Methods: Conventional and sexed frozen semen from nine bulls were used for IVF and AI.
Results: The IVF and AI conception rates of each bull were confirmed to be positively correlated between the conventional frozen and sexed frozen semen.