Advantages of aggregation-induced luminescence microspheres compared with fluorescent microspheres in immunochromatography assay with sandwich format.

Organic fluorescein dye-embedded fluorescent microspheres (FMs) are currently the most established commercially fluorescent markers, and they have been widely used to improve the sensitivity of immunochromatography assay (ICA). However, these FMs have natural defects, such as the aggregation-caused quenching effect and small Stokes shift, which are not conducive to improving the detection performance of ICA. Herein, two green emitted FMs, namely aggregation-induced emission FMs (AIEFMs) and fluorescein isothiocyanate FMs (FITCFMs), were prepared by swelling the AIE luminogens and FITC dyes into the carboxyl group-modified polystyrene microspheres.

Ultrabright orange-yellow aggregation-induced emission nanoparticles for highly sensitive immunochromatographic quantification of ochratoxin A in corn.

Herein, we report a novel aggregation-induced emission nanoparticles (AIENPs)-based immunochromatography assay (ICA) platform to detect ochratoxin A (OTA) using orange-yellow-emitting AIENPs as fluorescent nanoprobes. Immunochromatographic strip is used for the quantitative detection of OTA in crop matrix using AIENPs coupled with anti-OTA ascites. Under optimal conditions, AIENPs-ICA exhibits stronger signal output capacity and higher sensitivity than traditional gold nanoparticles-based ICA.