Global characterization of somatic mutations and DNA methylation changes during vegetative propagation in strawberries.

Somatic mutations arise and accumulate during tissue culture and vegetative propagation, potentially affecting various traits in horticultural crops, but their characteristics are still unclear. Here, somatic mutations in regenerated woodland strawberry derived from tissue culture of shoot tips under different conditions and 12 cultivated strawberry individuals are analyzed by whole genome sequencing. The mutation frequency of single nucleotide variants is significantly increased with increased hormone levels or prolonged culture time in the range of 3.

Factor analysis and GA-BP-ANN prediction of nitrogen diffusion behavior in underground laboratory under ventilation conditions.

Nitrogen is widely used in various laboratories as a suppressive gas and a protective gas. Once nitrogen leaks and accumulates in a such confined space, it will bring serious threats to the experimental staff. Especially in underground tunnels or underground laboratories where there is no natural wind, the threat is more intense.

Identification of high-risk patients for development of type B aortic dissection based on novel morphological parameters.

Predicting the development of sporadic type B aortic dissection (TBAD) always remains a difficult issue. This study aimed to identify high-risk patients for development of TBAD based on morphological parameters. This propensity-score-matched case-control study collected and reconstructed the computed tomography angiography of acute TBAD patients and hospital-based control participants without aortic dissection from January 2013 to December 2016.

Potential roles of acyl homoserine lactones (AHLs) in nitrifying bacteria survival under certain adverse circumstances.

Potential roles of quorum sensing (QS) in nitrifying bacteria activity and ecology, particularly under adverse circumstances have been rarely reported. Herein, eight lab-scale nitrification sequencing batch reactors, with or without adding acyl homoserine lactones (AHLs) were operated under adverse circumstances respectively. The results indicated that the introduction of AHLs significantly enhanced the nitrogen removal efficiency in the presence of nitrification inhibitors (dicyandiamide, DCD), accelerated the low temperature (10 °C) group into stable stage, and improved the utilization efficiency of AHLs in these two groups.

Molecular Dynamics Study on Hugoniot State and Mie-Grüneisen Equation of State of 316 Stainless Steel for Hydrogen Storage Tank.

To promote the popularization and development of hydrogen energy, a micro-simulation approach was developed to determine the Mie-Grüneisen EOS of 316 stainless steel for a hydrogen storage tank in the Hugoniot state. Based on the combination of the multi-scale shock technique (MSST) and molecular dynamics (MD) simulations, a series of shock waves at the velocity of 6-11 km/s were applied to the single-crystal (SC) and polycrystalline (PC) 316 stainless steel model, and the Hugoniot data were obtained. The accuracy of the EAM potential for Fe-Ni-Cr was verified.

The loss of helical flow in the thoracic aorta might be an identifying marker for the risk of acute type B aortic dissection.

Background And Objective: The occurrence of acute type B aortic dissection (TBAD) remained unclear. This study aimed to investigate the association between flow features and hemodynamic parameters in aortas that demonstrated the risk of TBAD occurrence.

Methods: The geometries of 15 hyperacute TBAD and 12 control patients (with healthy aorta) were reconstructed from computed tomography angiography images.

Effects of different bypass surgeries on LSA revascularization in patients with left subclavian occlusion.

Introduction: Left subclavian artery (LSA) bypass surgery is mainly carried out for patients with severe left subclavian occlusion. This article aimed to evaluate the hemodynamic effects of different surgical bypass modes on LSA revascularization.

Methods: Three-dimensional models of the aorta were reconstructed from CTA images of a patient with LSA occlusion, a patient with type B aortic dissection with LSA coverage during thoracic endovascular aortic repair, and a healthy 74-year-old man, resulting in six modes for each person: healthy LSA mode, LSA occlusion mode and four bypass modes.

Deciphering the Molecular Signatures Associated With Resistance to in Strawberry Flower by Comparative and Dynamic Transcriptome Analysis.

Gray mold caused by , which is considered to be the second most destructive necrotrophic fungus, leads to major economic losses in strawberry ( × ) production. preferentially infects strawberry flowers and fruits, leading to flower blight and fruit rot. Compared with those of the fruit, the mechanisms of flower defense against remain largely unexplored.

Function of quorum sensing and cell signaling in wastewater treatment systems.

Quorum sensing (QS) is a communication mode between microorganisms to regulate bacteria ecological relations and physiological behaviors, thus achieve the physiological function that single bacteria cannot complete. This phenomenon plays important roles in the formation of biofilm and granular sludge, and may be related to enhancement of some functional bacteria activity in wastewater treatment systems. There is a need to better understand bacterial QS in engineered reactors, and to assess how designs and operations might improve the removal efficiency.

Reproduction response of Colletotrichum fungi under the fungicide stress reveals new aspects of chemical control of fungal diseases.

Systemic fungicides and antifungals are used as frontline treatments for fungal diseases in plants and humans. It is generally accepted that fungicides will bring significant negative side-effects to the environment and result in fungicide resistance in the pathogenic fungi. Although previous research has focused on fungicide application rates and fungal resistance for a long time, little attention has been paid to fungicide residues after treatment, especially their potential role in fungal growth and sporulation.

Comparative transcriptome analysis reveals fruit discoloration mechanisms in postharvest strawberries in response to high ambient temperature.

The aim of this study was to examine both physiological and molecular evidences related to fruit discoloration in postharvest strawberries under high ambient temperature. The results showed the total anthocyanin and their main components in the strawberries under 35 °C were greatly increased due to the significant up-regulations of anthocyanin biosynthetic genes and transportation genes. High ambient temperature greatly improved the activities of peroxidase (POD) and enhanced gene expressions of , and .

Investigation of the Propagation of Stress Wave in Nickel-Titanium Shape Memory Alloys.

Based on irreversible thermodynamic theory, a new constitutive model incorporating two internal variables was proposed to investigate the phase transformation and plasticity behavior in nickel-titanium (NiTi) shape memory alloys (SMAs), by taking into account four deformation stages, namely austenite elastic phase, phase transition, martensitic elastic phase, and plastic phase. The model using the material point method (MPM) was implemented by the FORTRAN code to investigate the stress wave and its propagation in a NiTi rod. The results showed that its wave propagation exhibited martensitic and austenitic elastic wave, phase transition wave, and plastic wave.

[Effects of exogenous ABA and GA3 on sugar concentration in flesh of cara cara navel orange].

The concentration of glucose, fructose, sucrose and total sugar were determined after exogenous ABA and GA3 treatment during young period of fruit and before fruit coloring in flesh of Cara Cara Navel Orange. The results showed that 10 mg x L(-1) ABA treatment improved glucose, fructose and total sugar concentration significantly or very significantly, 50 mg x L(-1) ABA treatment improved sucrose concentration very significantly, but 100 mg x L(-1) ABA treatment reduced glucose concentration very significantly. GA3 treatment of lower and middle concentrations (10, 50 and 250 mg x L(-1)) improved sucrose concentration very significantly, 10 mg x L(-1) GA3 treatment had no remarkable effect on glucose and fructose concentration but improved total sugar concentration very significantly, GA3 treatment of 50, 250 and 500 mg x L(-1) decreased glucose, fructose and total sugar concentration very significantly.

[Effects of bagging on pigment, sugar and endogenous hormone contents of Cara Cara orange flesh].

This paper studied the effects of bagging during the period from young fruit formation to fruit coloration on the contents of pigment, sugar and endogenous hormone in Cara Cara orange flesh. The results showed that bagging could significantly increase the lycopene and beta-carotene contents of matured fruit, but didn't have any effects on its GA and ABA contents. The GA content decreased rapidly during fruit-expanding period, and maintained at a lower level in the period from fruit coloration to maturing.

[Expression of p15 protein in poorly differentiated nasopharyngeal carcinoma].

Objective: To explore the role of p15 protein in the pathogenesis of poorly differentiated nasopharyngeal carcinoma (NPC).

Method: Using immunohistochemical LSAB (Labeled Streptavidin Biotin method), we examined 55 samples of poorly differentiated NPC and 15 samples of nasopharyngeal normal tissue for expression of p15 protein.

Result: The p15 protein expression was located in the cytoplasm and nuclear membranes.