The effects of ethanol, 1,2-propanediol, and glycerol at concentrations from 10 % to 40 % on the thermal denaturation and aggregation of silver carp (Hypophthalmichthys molitrix) myosin were investigated. The results revealed that ethanol and 1,2-propanediol induced thermal denaturation of myosin more rapidly than glycerol, which minimally impacted the secondary structure. At 10 % concentration, 1,2-propanediol significantly influenced myosin's secondary structure more than ethanol.
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