Yeast culture is a complex fermentation product consisting of fermentation substrate, yeast cells and their metabolites. This study investigated the potential of yeast culture in replacing fishmeal in the diet of yellow catfish (). First, a basal diet was formulated to contain 160 g/kg fishmeal (CON), and then the dietary fishmeal was decreased to 120, 80, 40 and 0 g/kg via yeast culture inclusion, respectively, to form another four isonitrogenous and isolipidic diets (YC-12, YC-8, YC-4 and YC-0).
View Article and Find Full Text PDFCitric acid is an organic acid extensively used in feed industry, and AZOMITE is a hydrated aluminosilicate compound rich in rare earth elements and trace mineral elements. This study investigated the supplemental effects of AZOMITE and citric acid individual or in combination on the growth performance, intestinal microbiota, morphology, digestive enzyme activity, serum indexes, and disease resistance of juvenile largemouth bass. Six diets were designed, including the control diet (CON) and the five additive-supplemented diets with the addition of 4 or 8 g/kg citric acid (CA4, CA8), 3 g/kg AZOMITE (A3), and their combined addition as 4 g/kg citric acid + 1.
View Article and Find Full Text PDFThis study investigated the dietary effects of lipid and protein levels on growth performance, feed utilization, body composition, lipid metabolism, and antioxidant capacity of triploid rainbow trout, . A 3 × 2 two-factor design was conducted with three crude lipid levels of 4%, 9%, and 14% (L4, L9, and L14) and two crude protein levels of 44%, 49% (P44, P49). Therefore, a total of six diets were prepared as P44/L4, P44/L9, P44/L14, P49/L4, P49/L9, and P49/L14.
View Article and Find Full Text PDFThe purpose of this study was to develop the potential of cottonseed protein concentrate (CPC) and protein (CAP) in the diet of rainbow trout () by evaluating the effects of substituting fishmeal with a CPC and CAP mixture on growth performance, nutrient utilization, serum biochemical indices, intestinal and hepatopancreas histology. In a basal diet containing 200 g/kg fishmeal (Con), the mixture of CPC and CAP (1:1) was used to reduce dietary fishmeal to 150, 100, 50 and 0 g/kg, to form five diets with the same crude protein and crude lipid contents (CON, FM-15, FM-10, FM-5 and FM-0). Then, the five diets were fed to rainbow trout (35.
View Article and Find Full Text PDFThe study investigated the effects of dietary sodium butyrate (SB) on the growth performance, nutrient utilization, intestinal histology, and microbiota of rainbow trout (). A high fishmeal diet and a low fishmeal diet were formulated to contain 200 g/kg or 100 g/kg fishmeal, respectively. Coated SB (50%) was supplemented to each of the diets at levels of 0, 1.
View Article and Find Full Text PDFClostridium autoethanogenum protein (CAP) is a new single-cell protein explored in aquatic feeds in recent years. This study investigated the dietary effects of CAP replacing fishmeal (FM) on the growth, intestinal histology and flesh metabolism of largemouth bass (Micropterus salmoides). In a basal diet containing 700 g/kg of FM, CAP was used to substitute 0%, 15%, 30%, 45%, 70% and 100% of dietary FM to form six isonitrogenous diets (Con, CAP-15, CAP-30, CAP-45, CAP-70, CAP-100) to feed largemouth bass (80.
View Article and Find Full Text PDFThis study compared the growth, flesh quality and metabolomics of largemouth bass () fed trash fish and formula feeds. Trash fish (TF), self-made feed (SF) and commercial feed (CF) were prepared with crude protein levels of 172.2 g/kg, 503.
View Article and Find Full Text PDFThis aim of this study was to investigate the effects of dietary aucubin on the growth, flesh quality, and metabolomics of grass carp (Ctenopharyngodon idella). Five diets were designed with the aucubin inclusion of 0 (control diet), 0.2, 0.
View Article and Find Full Text PDFThe objective of this study was to investigate the dietary effects of Eucommia ulmoides bark and leaf (EB, EL) supplementation on the growth, lipid metabolism, flesh quality, and transcriptome of grass carp (Ctenopharyngodon idellus). EB and EL were individually added to the basal diet (control) at concentrations of 20 g/kg and 40 g/kg, respectively, and then the three diets were fed to grass carp (59.7 ± 0.
View Article and Find Full Text PDFTwo experiments were conducted to investigate the in vitro effects of Eucommia ulmoides (E. ulmoides) and its active components on the growth, lipid metabolism and collagen metabolism of grass carp's (Ctenopharyngodon idellus) hepatocytes and intramuscular fibroblasts. In experiments 1 and 2 (Expt.
View Article and Find Full Text PDFThe study investigated the dietary effects of Clostridium autoethanogenum protein (CAP) substituting fish meal on the growth, intestinal histology, serum immune indexes and transcriptome of Pacific white shrimp, Litopenaeus vannamei. Four isonitrogenous and isolipidic diets were designed as the control diet (CON) containing 560 g/kg fish meal, and three fish meal-substituted diets in which 30% (CAP-30), 45% (CAP-45) and 70% (CAP-70) fish meal were replaced with CAP, respectively. The four diets were fed to shrimp with initial body weight of 2.
View Article and Find Full Text PDFWe previously demonstrated that K17, isolated from Korean kimchi, has high antioxidant levels in vitro and in vivo. However, its effect on is unknown. In this study, we investigated the impact of K17 supplementation on the lipid metabolism, antioxidant response, liver histology, and fillet quality of .
View Article and Find Full Text PDFCreatine improves flesh quality on mammalian but studies on crustaceans are scarce. In the present study, diets with six levels of creatine (1.23, 2.
View Article and Find Full Text PDFThe present study aims to investigate the effects of dietary synbiotics supplementation methods on growth, feed utilization, hepatopancreas and intestinal histology, non-specific immunity and microbiota community of Pacific white shrimp (Litopenaeus vannamei). A control diet was designed to contain 18% fish meal (CON), and then 3 g kg synbiotics (Bioture, consisting of Bacillus subtilis, Saccharomyces cerevisiae, β-glucan and mannan oligosaccharide, etc) was supplemented to the control diet with three methods, directly adding in diets for pelleting (DAP), spraying diets after pelleting at once (SDA), spraying diets before feeding every day (SDE). Shrimp with initial body weight of 1.
View Article and Find Full Text PDFAzomite is a hydrated calcium sodium aluminosilicat rich in rare earth elements. To investigate the dietary effects of Azomite on growth, intestine microbiota and morphology, immunohematological changes and disease resistance, seven diets with Azomite supplementation of 0 (the control), 1.0, 2.
View Article and Find Full Text PDFConnective tissue growth factor (ctgf) is involved in the proliferation, migration, adhesion of cell, and the constituent of extracellular matrix, which plays an important role in embryogenesis, angiogenesis, wound repair, and fibrosis diseases. In this study, the cDNA sequence of grass carp ctgf gene was cloned by rapid amplification of cDNA ends (RACE) method; then, the characteristics of this gene and the predicted protein sequence were analyzed by bioinformatics methods, and the tissue differential expression pattern was detected by the quantitative real-time PCR. The results showed that the grass carp ctgf gene has a full-length of 2223 bp, encoding 343 amino acids.
View Article and Find Full Text PDFUnc45 myosin chaperone b(unc45b)gene is a molecular chaperone that mediates the folding, assembly and accumulation of thick-filament myosin in the formation of sarcomere, which plays an important role in the development of striated muscle and the stability of sarcomere. In this study, the complete cDNA sequence of unc45b gene of grass carp was obtained by rapid amplification of cDNA ends (RACE), and the characteristics of the unc45b protein predicted from gene sequence was analyzed by bioinformatics methods. The differential expression pattern in tissues was also detected by quantitative real-time PCR.
View Article and Find Full Text PDFMitochondrial DNA B Resour
April 2016
In this study, the complete mitogenome sequence of the broad-nosed pipefish (Linnaeus, 1758) () has been sequenced by next-generation sequencing method. The assembled mitogenome consisting of 16,488bp, includes 13 protein coding genes, 22 transfer RNAs and 2 ribosomal RNAs genes. The overall base composition\ of \the broad-nosed pipefish is 27.
View Article and Find Full Text PDFMitochondrial DNA A DNA Mapp Seq Anal
November 2016
In this study, the complete mitogenome sequence of the big-belly seahorse, Hippocampus abdominalis (Lesson, 1827) (Syngnathiformes: Syngnathidae), has been sequenced by the next-generation sequencing method. The assembled mitogenome is 16 521 bp in length which includes 13 protein-coding genes, 22 transfer RNAs, and 2 ribosomal RNAs genes. The overall base composition of the seahorse is 31.
View Article and Find Full Text PDFMitochondrial DNA A DNA Mapp Seq Anal
September 2016
In this study, the complete mitogenome sequence of Protomelas annectens Regan 1922 has been sequenced by next-generation sequencing method. The assembled mitogenome consisting of 16 583 bp, includes 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and 1 control region. The overall base composition of P.
View Article and Find Full Text PDFJ Anim Sci Biotechnol
January 2014
Background: This study was to determine if feeding laying hens with defatted diatom microalgal biomass (DFA) from biofuel production affected their egg production and health status.
Methods: Five replicates of 5 individually caged ISA Babcock White leghorn hens were fed 4 diets, including a corn-soybean meal control diet, a diet containing 7.5% DFA substituting for soybean meal, and diets containing 7.