[Analysis of the osteogenetic effects exerted on mesenchymal stem cell strain C3H10T1/2 by icariin via MAPK signaling pathway in vitro].

Objective: To investigate the effects of icariin, an effective extract from traditional Chinese medicine Epimedium pubescens with the function of tonifying kidney, in promoting osteogenesis of mesenchymal stem cell line C3H10T1/2, and to explore the underlying mechanism.

Methods: After culture with icariin (0, 10(-7), 10(-6), 10(-5), and 10(-4) mol/L) and osteogenic supplement for 26 d in vitro, osteogenic differentiation of C3H10T1/2 cells was detected by alkaline phosphatase (ALP) assay. The RNA was extracted from cells cultured with 10(-5) mol/L icariin for 2, 8, 24 and 48 hours, and mRNA expressions of p38, p42 and p44 were measured using real-time reverse transcription-polymerase chain reaction (PCR) method.

[Prognosis and its affecting factors in children with acute respiratory distress syndrome].

Objective: To study the prognosis and the factors affecting the prognosis in children with acute respiratory distress syndrome (ARDS).

Methods: Seventy-eight children with ARDS were enrolled. The states of their survival within 30 days were followed-up.