[p16INK4a expression mediated by recombinant adenovirus can induce senescence of A549 cells].

Objective: To construct E1-deletion and replication-defective human type 5 recombinant adenovirus vector and to study the effect of p16INK4a on proliferation and aging of A549 cells.

Methods: p16INK4a cDNA was cloned into pAdCMV to construct recombinant pAdCMV p16INK4a, which was co-transfected into 293 cell together with pJM17. The recombinant p16INK4a adenovirus (Ad-p16INK4a) was generated by homologous recombination and identified with duplex PCR.

[Significance of imbalance between matrix metalloproteinases and tissue type inhibitor of metalloproteinases in renal tubulointerstitial lesions of aging rats].

Objective: To explore the imbalance between the expression of metalloproteinases (MMPs) and that of tissue type inhibitors of metalloproteinase (TIMPs) in the renal tubulointerstitial lesions of aging rats and the potential role of this imbalance.

Methods: Forty-eight male 26-month-old Wistar rats were randomly divided into 2 groups of 24 rats: unilateral ureteral obstruction (UUO) group with the left ureter ligated and excised and false operation group used as control group. Forty-eight male 3-month-old Wistar rats were randomly divided into 2 groups of 24 rats: UUO group and false operation group just as in the 26-month-old rats.

[Multicenter prospective study on relationship of TCM syndrome type and renal pathology in 286 patients with IgA nephropathy].

Objective: To study the distribution pattern of TCM Syndrome type and its relationship with renal pathology in patients with IgA nephropathy.

Methods: Apopting multicenter coordinated method, patients' TCM Syndrome type was differentiated according to their clinical manifestations, data concerning laboratory examination and renal pathology were collected to establish a database for analyzing the relationship between TCM Syndrome type and renal pathology in 286 patients.

Results: Patients of Pi-Fei Qi-deficiency type (type 1) and both Qi-Yin deficiency type (type 2) showed rather milder pathological changes, by Lee classification, most of them belonged to grade I-III (72.

A novel angiotensin II type 1 receptor-associated protein induces cellular hypertrophy in rat vascular smooth muscle and renal proximal tubular cells.

Angiotensin II stimulates cellular hypertrophy in cultured vascular smooth muscle and renal proximal tubular cells. This effect is believed to be one of earliest morphological changes of heart and renal failure. However, the precise molecular mechanism involved in angiotensin II-induced hypertrophy is poorly understood.

[Na+/dicarboxylate cotransporter expression changed with aging in basolateral membrane of the renal tubular epithelial cells].

Objective: To study the change with aging of Na(+)/dicarboxylate cotransporter (NaDC3) expression in basolateral membrane of epithelial cell in proximal renal tubules in rat and human being.

Methods: Six groups of 6 Wistar rats at the age of: 1 day, 7 days, 1 month, 3 months, 12 months, and 24 months respectively were killed and their kidneys were taken to be examined immunohistochemically. Samples of kidney from 15 normal human beings, 5 in the group aged 6 - 8, 5 in the group aged 32 - 44, and 5 in the group aged 63 - 65, were examined immunohistochemically.

[Changes of STAT3 in cell replicative senescence and effects of angiotensin II on STAT3].

Objective: To investigate the changes of STAT3 in cell replicative senescence and the effects of angiotensin II (AngII) on STAT3.

Methods: Normal human fetal lung diploid fibroblast cell line WI-38 was cultured and the senescent cell was defined with beta-gal staining. Electrophoretic mobility shift assay and Western blotting assay were used to detect the activation of STAT3 during cell replicative senescence and examine the process of STAT3 activation with AngII.

[Significance of vascular lesions in IgA nephropathy and their influencing factors, a study of 1,005 cases].

Objective: To analyze the characteristics of vascular lesions and their influencing factors in IgA nephropathy (IgAN).

Methods: The clinical and pathologic materials of 1,005 IgAN patients were analyzed.

Results: Of the 1005 IgAN patients 54.

[Clinical observation on effect of shenle capsule in treating mesangial proliferating glomerulonephritis].

Objective: To study the therapeutic effect of Shenle capsule (SLC) in treating mesangial proliferating glomerulonephritis (MsPGN) and to explore its therapeutic mechanism and clinical indication.

Methods: Adopting case control method, taking angiotensin-converting enzyme inhibitor (benazepril) as control agent, the 142 cases of MsPGN were randomly divided into 3 groups, treated with SLC (Group A, n = 36), SLC plus benazepril (Group B, n = 68) and benazepril alone (Group C, n = 38) respectively. Changes of fibrinogen, lipids, renal function and urinary protein were observed.