[A multicenter survey of antibiotic use in very and extremely low birth weight infants in Hunan Province].

Objective: To investigate the current status of antibiotic use for very and extremely low birth weight (VLBW/ELBW) infants in neonatal intensive care units (NICUs) of Hunan Province.

Methods: The use of antibiotics was investigated in multiple level 3 NICUs of Hunan Province for VLBW and ELBW infants born between January, 2017 and December, 2017.

Results: The clinical data of 1 442 VLBW/ELBW infants were collected from 24 NICUs in 2017.

21-gene recurrence score and adjuvant chemotherapy decisions in patients with invasive lobular breast cancer.

Aim: To determine the effect of the 21-gene recurrence score (RS) on outcome and chemotherapy decision in breast invasive lobular carcinoma (ILC).

Materials & Methods: We included 6467 patients with early stage and estrogen receptor-positive ILC from the Surveillance, epidemiology, and end results database.

Results: A total of 9.

Expression of pro-inflammatory cytokines in the auditory cortex of rats with salicylate-induced tinnitus.

Tinnitus often results in severe psychological distress. The present study hypothesized that tinnitus acts as a chronic stressor and induces dysregulation of the production of cytokines. The gap pre‑pulse inhibition of acoustic startle paradigm was applied to test tinnitus‑like behavior in rats.

[Protective Effect of Lycium ruthenicum on Peripheral Blood System Against Radiation Injury in Mice].

Objective: To study the protective effect of Lycium ruthenicum on peripheral blood system against radiation injury in mice.

Methods: Kunming mice were randomly divided into control group, model group, positive group and Lycium ruthenicum high dose (8 g/kg), middle dose (4 g/kg) and low dose (2 g/kg)treatment groups that experimented three days after irradiation. In the same way, groups were set at 7 days and 14 days after irradiation respectively.

[Expression and significance of Twist1 and MMP-2 in endometrial endometrioid adenocarcinoma].

Objective: To investigate the expression of Twist1 and MMP-2 protein and their significance in endometrial endometrioid adenocarcinoma.

Methods: The expression of Twist1 and MMP-2 protein in 70 cases of endometrial endometrioid adenocarcinoma was detected on tissue chips using immunohistochemical staining.

Results: The positive rates of Twist1 and MMP-2 protein expression were 65.

Quantification of andrographolide sodium bisulphite in urine after intravenous injection to rats by LC-MS/MS.

A liquid chromatography-tandem mass spectrometry method for the determination of andrographolide sodium bisulphite (ASB) in rat urine was established and validated. To our knowledge, the analytical method is the first developed assay for the determination of ASB in urine samples. Dehydroandrographolide (DAG) was used as an internal standard.

[Investigation of birth state and disease spectrum in 1,434 hospitalized neonates].

Objective: To investigate the birth state of neonates and the disease spectrum of hospitalized neonates from a primary hospital, and compare with the national data of the same period.

Methods: A retrospective investigation was carried out in 1,434 neonates born or hospitalized in this hospital from January 2005 to December 2005.

Results: During the investigation period, there were 1,100 neonates born in the department of obstetrics.

[Correlation of vascular endothelial growth factor D, microlymphatic density and microvessel density with development and metastasis of rectal cancer].

Objective: To investigate the correlation of vascular endothelial growth factor D (VEGF-D) expression, microlymphatic density (MLD) and microvessel density (MVD) levels with the development and metastasis of rectal cancer.

Methods: Eighty specimens from resected middle-lower rectal cancer diagnosed by pathology were examined by immunohistochemistry for VEGF-D,MLD and MVD. Simultaneously, 40 biopsy specimens from rectal polyps and 80 specimens from normal rectal tissue were examined as controls.

[Study of quality on rhubarb from different habitats in Qinghai Province].

Objective: To investigate the effect of different habitats on rhubarb quality.

Method: The rhubarb samples from various parts of Qinghai province were analysed by fingerprint.

Results And Conclusion: The distribution and quality of wild Tangute rhubarb is better than Palmate sorrel rhubarb.

Antiviral therapeutic efficacy of foscarnet in hepatitis B virus infection.

Foscarnet (PFA), a viral DNA polymerase inhibitor, is a clinical agent for herpes viruses. The goal of the study was to evaluate the therapeutic efficacy of PFA in hepatitis B virus (HBV) infection. Intravenous infusion of PFA (1 g/day) for 4 weeks significantly reduced serum HBeAg (p<0.

Synthesis of 1-(3-phthalimido-2-oxobutyl)-4-substituted- phenylpiperazines and their anti-HIV reverse transcriptase activity.

Aim: Synthesis of 1-(3-phthalimido-2-oxobutyl)-4-substituted- phenylpiperazines (5-15).

Methods: The starting material nitrogen mustard hydrochloride (16), reacted with the corresponding substituted anilines to afford piperazine hydrochlorides (17-27), which were then coupled with 1-bromo-3-phthalimidobutan-2-one (4) to give the target compounds.

Results: Eleven target compounds (5-15) were synthesized, which were characterized by 1HNMR, IR and elemental analysis.