Benefit of HSP90α intervention on ischemia-reperfusion injury of venous blood-congested flaps.

In order to decrease the incidence of flap necrosis after reconstructive surgeries, new approaches are required. In the present study, a model of venous congested flaps in rats was established to test the heat shock protein (HSP) 90α, 'F-5', protein as an intervention therapy to alleviate ischemia-reperfusion injury. A recombinant plasmid pET15b-F-5 carrying the gene was constructed and the induced protein was purified from bacterial cell cultures.

[Role of lymphatics in bacterial translocation from intestine in burn rats].

Objective: To investigate the role of lymphatics in bacterial translocation from intestine of rats with burn.

Methods: Escherichia coli (E. coli) labeled with chloromethylbenzamidodialkylcarbocyanine (CM-DIL) were prepared.

[Expression of tumor necrosis factor related apoptosis inducing ligand and its receptor in thymus during early post-burn stage in rat with severe burns].

Objective: To investigate the effect of tumor necrosis factor related apoptosis inducing ligand (TRAIL) and its receptor on apoptosis in thymus during early post-burn stage in rat with severe burns.

Methods: Fifty Wistar rats were randomly divided into sham scald group (SS, n = 10) and burn group (n = 40). The apoptosis in thymus in rats was detected with annexin V/FITC-PI double staining at 4, 12, 24, 48 post-burn hours (PBH).

Lymph invasion route of bacteria and endotoxin and CD4+/CD8+ T cells ratio changes of draining lymph node of burn infection wound.

The aim of this study was to determine the lymphatic invasion route of bacteria and endotoxin of burn wounds infected by Pseudomonas aeruginosa and moreover, the effect of P. aeruginosa infection of the burn wound on the draining lymph node and lymph fluid. Male Wistar rats were subjected to unilateral hind limb burn+wound infected by P.

Changes in cytokine levels and CD4+/CD8+ T cells ratio in draining lymph node of burn wound.

The aim of this study was to determine the changes of cytokine levels in draining lymph fluid and the changes of CD4+/CD8+ T-cells ratio in draining lymph node of burn wound. Male Wistar rats were subjected to unilateral hind limb burn (burn limbs group) and contralateral hind limb without burn (control limbs group). On hours 6, 24, and 72 after burn, rats were killed; lymph fluid in the efferent lymph trunk of the common iliac lymph nodes (CILN) were collected; and lymph fluid Interferon-gamma (IFN-gamma), interleukin-4 (IL-4) and tumor necrosis factor-alpha (TNF-alpha) concentrations were measured by enzyme-linked immunosorbent assay.

[Expression and significance of TRAIL receptors in fibroblasts of hypertrophic scar].

Objective: To detect the expression of TRAIL receptors in fibroblasts of hypertrophic scar in the proliferative stage and explore its significance.

Methods: 30 samples of hypertrophic scar were taken from 30 burn cases in the proliferative stage. 30 samples of normal skin were taken as the control.

[The changes in the tumor necrosis factor alpha, interleukin-6 and interleukin-8 levels in the lymph and of the dynamics of the lymphokines during shock stage of rats with major burns].

Objective: To study the dynamic changes in the lymphokines and the changes in tumor necrosis factor alpha (TNF-alpha), interleukin-6 (IL-6), interleukin-8 (IL-8) levels in the lymph during shock stage of rats with major burns.

Methods: Forty-two male adult Wistar rats were randomly divided into burn resuscitation group (A, n = 18), burn non-resuscitation (B, n = 18) and the control (C, n = 6) groups. The TNF-alpha, IL-6 and IL-8 levels in the lymph were determined with radioimmunoassay at 6, 24, 48 postburn hours (PBH).