Fast and sensitive smartphone colorimetric detection of whole blood samples on a paper-based analytical device.

Background: Methods based on paper-based analytical devices (PAD) and smartphone photographic colorimetric detection have become representative instrument-independent point-of-care testing (POCT) platforms due to their low cost and simplicity. However, the detection of target components from whole blood sample still presents challenges in terms of field preparation of small amounts of blood sample and detection sensitivity. This paper presents a rapid online processing method for whole blood samples on PAD based on plasma separation membrane (PSM), and combined with electrokinetic stacking and selective chromatic reaction.

Paper-based sample processing for the fast and direct MS analysis of multiple analytes from serum samples.

Amino acids are closely related to human health, and their rapid determination is important for the rapid diagnosis, timely treatment, and assessment of serious diseases. In this work, we propose a novel paper-based sample-processing device combined with isotope-dilution MS for the fast analysis of 11 amino acids from blood samples. By using an isoelectric focusing electrokinetic separation method, without the aid of carrier ampholytes and the addition of inhibitors, this approach uses only the characteristic of the isoelectric point of the target amino acids to achieve separation and purification from other coexisting components in the medium; it can meet the requirements for mass spectrometry detection.

[Effect of neuronal differentiation induced by nerve growth factor on the tolerance-dosage of ultraviolet radiation of PC12 cells].

Objective: To evaluate the effect of neuronal differentiation induced by nerve growth factor (NGF) on the tolerance-dosage of ultraviolet radiation of PC12 Cells.

Methods: Neuron-differentiated PC12 cells and untreated PC12 cells were exposed to different ultraviolet radiation dosage of 10, 30, 60, 80, 100, and 200 mJ/cm2. Cell survival rates were determined by MTT assay.

[N2O emission from rice-rapeseed rotation system in Chengdu Plain of Sichun Basin].

By using static chamber/gas chromatograph techniques, the N2O emission from rice-rapeseed rotation system in Chengdu Plain of Sichuan Basin was measured from June 2005 to June 2006, with its characteristics and affecting factors investigated. The results showed that the total emission of N2O in a rotation cycle was (8.3 +/- 2.

Numerical calculation of the sound field focused by acoustic lens with an arbitrary axisymmetric sound speed distribution.

The expressions that describe the sound field focused by an acoustic lens with different sound speed distribution (SSD) in lens are given. Numerical calculation results are presented, in which the axial and lateral sound pressure distributions and the -3 dB sound beam profile along the focused field are given out for three types of the acoustic focused lens with revolving-arc curved outer surface and four types' axisymmetric SSD. It is shown that, when sound beam is focused by a lens with SSD, the best focal point moves away from the lens and achieves a large focal region compared with the no-SSD case.