Exosomes from artesunate-treated bone marrow-derived mesenchymal stem cells transferring to promote osteogenesis via TAF15-RUNX2 pathway.

Effect of artesunate (ART)-treated bone marrow-derived mesenchymal stem cells-derived exosomes (BMSC-Exos) on osteogenesis and its underlying mechanisms were investigated. Proliferation, alkaline phosphatase activity and calcified nodule formation of osteoblasts were determined. A mouse model of osteoporosis was established by ovariectomy.

Norepinephrine Induces PTSD-Like Memory Impairments Regulation of the β-Adrenoceptor-cAMP/PKA and CaMK II/PKC Systems in the Basolateral Amygdala.

Glucocorticoids (GCs) can modulate the memory enhancement process during stressful events, and this modulation requires arousal-induced norepinephrine (NE) activation in the basolateral amygdale (BLA). Our previous study found that an intrahippocampal infusion of propranolol dose-dependently induced post-traumatic stress disorder (PTSD)-like memory impairments. To explore the role of the noradrenergic system of the BLA in PTSD-like memory impairment, we injected various doses of NE into the BLA.

Effects of triclosan on performance, microbial community and antibiotic resistance genes during partial denitrification in a sequencing moving bed biofilm reactor.

Effects of triclosan (TCS) on performance, microbial community and antibiotic resistance genes (ARGs) during partial denitrification (PD) were investigated in a sequencing moving bed biofilm reactor (SMBBR). TCS inhibited nitrite accumulation; inhibition effect was more obvious as TCS concentration increased from 1 to 5 mg/L, but it could recover. Extracellular polymeric substances contents increased with 1 mg/L TCS addition and decreased a lot at 5 mg/L TCS.

Performance of magnetic graphene oxide/diethylenetriaminepentaacetic acid nanocomposite for the tetracycline and ciprofloxacin adsorption in single and binary systems.

Adsorption of tetracycline (TC) and ciprofloxacin (CIP) in single and binary systems by diethylenetriaminepentaacetic acid-functionalized magnetic graphene oxide (DDMGO) was explored. DDMGO were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and zeta potential measurements. The adsorption kinetics and equilibrium data of single antibiotic were well fitted by pseudo-second-order and Freundlich isotherm models, respectively.

Propranolol can induce PTSD-like memory impairments in rats.

Introduction: One hallmark symptom of post-traumatic stress disorder (PTSD) is an inability to restrict fear responses to the appropriate predictor. An infusion of glucocorticoids (GCs) after a high-intensity shock has been shown to induce PTSD-like memory impairments. In addition to GCs, noradrenergic signalling is also recognized as a key biomarker underlying PTSD symptomatology.

[Analysis of minocycline hydrochloride combined with Vitapex in treating senile chronic periodontal-endodontic combined lesions].

Purpose: To investigate the effect of minocycline hydrochloride combined with Vitapex in treating senile chronic periodontal-endodontic combined lesions.

Methods: One hundred twenty patients with senile chronic periodontitis and endodontic lesions patients treated in our hospital between Jun.2012 to Jun.

[Pilot evaluation of masticatory efficiency and patients' satisfaction in long-centric occlusal pattern complete denture wearers].

Objective: To assess the masticatory efficiency and patient' satisfaction in long-centric occlusal pattern complete denture wearers.

Methods: The anatomic occlusal pattern and long-centric occlusal pattern complete dentures were made for each of 10 edentulous patients with severe alveolar rigde absorption simultaneously using the technique of interchangeable artificial teeth. The order of delivery of different occlusal pattern complete denture was determined according to randomized principle.

[Influence of experimental palatal plate on mandibular position during continuous [n] phonation in complete denture wearers].

Objective: To clarify the influence of an experimental palatal plate on the mandibular position during continuous [n] phonation and at the physiological rest position in complete denture wearers.

Methods: Ten complete denture wears volunteers were investigated. Two kinds of experimental palatal plate with a thickness of 3 mm and 5 mm (from the denture basal surface to denture polished surface) were fabricated and used for each subject.

[Change of polished surface contours on mandibular complete denture base in different residual ridge resorption situation: a pilot study].

Objective: To investigate the profile of mandibular denture polished surface in edentulous patients with different residual ridge resorption (RRR) using neutral zone record.

Methods: Twenty randomly selected edentulous patients with different category of RRR were investigated, which were divided into three groups according to ridge resorption grade and the relationships between upper and lower jaws: group 1: Class I edentulous patients (n = 6), group 2: Class II edentulous patients (n = 7), group 3: Class III edentulous patients and crossbite jaw relationship (n = 7). All subjects were asked to perform sufficient functional movement with silicon registration material in their mouth to record the shape, which were interpreted as the ideal shape of denture polished surface.

Update of TTD: Therapeutic Target Database.

Increasing numbers of proteins, nucleic acids and other molecular entities have been explored as therapeutic targets, hundreds of which are targets of approved and clinical trial drugs. Knowledge of these targets and corresponding drugs, particularly those in clinical uses and trials, is highly useful for facilitating drug discovery. Therapeutic Target Database (TTD) has been developed to provide information about therapeutic targets and corresponding drugs.

Pathway sensitivity analysis for detecting pro-proliferation activities of oncogenes and tumor suppressors of epidermal growth factor receptor-extracellular signal-regulated protein kinase pathway at altered protein levels.

Background: Mathematic models and sensitivity analyses of biologic pathways have been used for exploring the dynamics and for detecting the key components of signaling pathways.

Methods: The authors previously developed a mathematic model of the epidermal growth factor receptor-extracellular signal-regulated protein kinase (EGFR-ERK) pathway using ordinary differential equations from existing EGFR-ERK pathway models. By using prolonged ERK activation as an indicator that may lead to cell proliferation under certain circumstances, in the current study, a pathway sensitivity analysis was performed to test its capability of detecting pro-proliferative activities through altered protein levels to examine the effects on ERK activation.

[Clinical evaluation of the effect of gold alloy and Ni-Cr alloy porcelain fused metal crown restorations].

Purpose: To observe the clinical effect of gold alloy porcelain fused metal (PFM) crown restoration and Ni-Cr alloy PFM crown restoration.

Methods: A total of 168 teeth from 48 patients were restored with gold alloy PFM crown. The other 48 patients, with a total of 179 teeth were restored with Ni-Cr alloy PFM crown.

Clinical presentation and treatment of distal posterior inferior cerebellar artery aneurysms: report on 5 cases.

Background: Aneurysms of the PICA are uncommon. Most of them arise at the PICA origin from the VA, whereas distal PICA aneurysms are exceptional. A retrospective analysis of 457 patients with SAH treated in our hospital found 5 patients with 6 distal PICA aneurysms (approximately 1% of SAHs).

Biomarkers responses of the earthworm Eisenia fetida to acetochlor exposure in OECD soil.

To examine the potential of a suite of biomarkers as early warning indicators of environmental pollution, sperm count, neutral red retention time (NRRT) and DNA damage were measured in earthworm Eisenia fetida exposed to increasing concentrations of acetochlor in OECD soil. The neutral red retention time of earthworms coelomocytes was sensitive to acetochlor pollution, and decreased significantly when the concentration was more than 10mgkg(-1) after 30 and 60 days of exposure (P<0.05).

Effect of herbicide acetochlor on cytochrome P450 monooxygenases and GST of earthworms Eisenia fetida.

To assess the sublethal toxicity of the herbicide acetochlor to earthworms and to find out biomarkers possible inducted under acetochlor exposure, Eisenia fetida was exposed to artificial soils supplemented with different concentrations of acetochlor(5, 10, 20, 40 and 80 mg/kg soil). Effects of the acetochlor on cytochrome P450 monooxygenases p-nitroanisole O-demethylase (ODM), aldrin epoxidase (AE) and glutathione-S-transferases (GSTs) activities were determined. The results revealed cytochrome P450 monooxygenases were elevated with increasing concentrations of acetochlor, and the AE activity increased significantly compared with control at the concentration of 80 mg/kg (P < 0.

[Factors influencing the activity of fibrinolytic enzymes from earthworm, Eeisenia foetida].

Objective: To study the factors influencing the activity of fibrinolytic enzymes from earthworm and to obtain the better way to extract fibrinolytic enzymes as well as keep its optimum activity.

Method: 75% alcohol, 0.9% NaCl and 10% saccharose was used to extract the crude fibrinolytic enzymes from earthworm, the method of urokinase gelose-fibrin plate was used to measure the activity of fibrinolytic enzymes from earthworm.