Relationship between Internet use and offline leisure activities among Chinese older adult people: a moderated mediation model.

Background: The process of aging in Chinese society is accompanied by the concurrent development of the Internet. In recent years, the influence of Internet use on the activities of older adults has attracted growing interest and is now a significant focus of both public health and gerontological research. Nevertheless, there is a need for further empirical research in this area.

Trends and advances in , a bibliometric analysis.

Background: Leptospirosis is an acute zoonotic disease caused by pathogenic , primarily transmitted to humans through contact with water or soil contaminated by the bacteria. It is globally distributed, with heightened prevalence in tropical regions. While prior studies have examined the pathophysiology, epidemiology, and risk factors of leptospirosis, few have explored trends and emerging topics in the field.

Recent advances in bacterial outer membrane vesicles: Effects on the immune system, mechanisms and their usage for tumor treatment.

Tumor treatment remains a significant medical challenge, with many traditional therapies causing notable side effects. Recent research has led to the development of immunotherapy, which offers numerous advantages. Bacteria inherently possess motility, allowing them to preferentially colonize tumors and modulate the tumor immune microenvironment, thus influencing the efficacy of immunotherapy.

Association between HALP (hemoglobin, albumin, lymphocyte, and platelet) score and poor outcomes in acute ischemic stroke patients with type 2 diabetes mellitus: a study from the Third China National Stroke Registry.

Background: The combined index (HALP) of hemoglobin, albumin, lymphocytes, and platelets is considered a novel scoring system that reflects systemic inflammation and nutritional status. This study aimed to investigate the relationship between HALP scores and poor outcomes in acute ischemic stroke (AIS) patients with type 2 diabetes mellitus (DM).

Methods: Patients with AIS and type 2 DM were screened from the Third China National Stroke Registry (CNSR-III) and divided into quartiles based on their HALP scores at admission.

Effects of antibiotic therapy on the early development of gut microbiota and butyrate-producers in early infants.

Background: Antibiotics, as the most commonly prescribed class of drugs in neonatal intensive care units, have an important impact on the developing neonatal gut microbiota. Therefore, comprehending the effects of commonly used antibiotic therapy on the gut microbiota and butyrate-producers in early infants could provide information for therapeutic decision-making in the NICU.

Objectives: To explore the effects of antibiotic therapy on the early development of gut microbiota and butyrate-producers in early infants.

Photoswitchable Branched Polyurethanes Based on Hexaarylbiimidazole for Photolithography.

Hexaarylbiimidazole (HABI) molecules have awakened a broad interest in photo-processing, super-resolution imaging, photoinduced self-healing materials, and photomechanical hydrogels due to their excellent photosensitivity and photo-induced cleavage properties. In this work, a novel photoswitchable branched polyurethanes (BPU), which are synthesized by copolymerizing HABI with glycerol, isophorone diisocyanate (IPDI), and polyethylene glycol (PEG), is designed. 7-Diethylamino-4-methylcoumarin (DMCO) is introduced as a radical quencher, which can not only avoid the hydroxyl interfering from conventional radical scavengers during the polymerization process but also promote efficient quenching of TPIR radicals.

Downregulation of hnRNPA1 inhibits hepatocellular carcinoma cell progression by modulating alternative splicing of ZNF207 exon 9.

Introduction: Hepatocellular carcinoma (HCC) is the most prevalent liver cancer and a leading cause of cancer-related deaths worldwide. Heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) plays a critical role in RNA metabolism, including alternative splicing, which is linked to cancer progression. Our study investigated the role of in HCC and its potential as a therapeutic target.

TAT-N24 enhances retinal ganglion cell survival by suppressing ZBP1-PANoptosome-mediated PANoptosis in an acute glaucoma mouse model.

The abrupt and substantial elevation of intraocular pressure (IOP) in acute glaucoma induces retinal ischemia/reperfusion (I/R) injury, resulting in progressive retinal ganglion cell (RGC) death and irreversible visual impairment. PANoptosis, a form of regulated cell death consisting of pyroptosis, apoptosis and necroptosis, is reported to be involved in high IOP-induced RGC death. However, the precise mechanisms of RGC death remain unclear, and neuroinflammation is considered to play a vital role.

Rad5 and Ubc4 directly ubiquitinate PCNA at Lys164 in vitro.

Ubiquitination of the proliferating cell nuclear antigen (PCNA) by the budding yeast protein Rad5 have important functions in replication stress responses. Rad5 together with the Ubc13-Mms2 complex attaches Lys63-linked ubiquitin chain to a highly conserved Lys164 residue in PCNA. The reaction requires prior PCNA mono-ubiquitination by the Rad6-Rad18 complex and signals for error-free DNA damage tolerance responses.

The overestimated risks for imported waste paper: Based on grey interval multi-criteria decision making under uncertainties.

China has prohibited the imported waste paper (IWP) from 2021 due to environmental burdens, its reasonability was doubtful owing to lacking a comprehensive evaluation. Previous studies just assessed the potential risks caused by pollutants from the upstream processes, ignoring the actual risks from the actual production processes, especially the generated toxic pollutants (heavy metals, PCDD/Fs, PAEs and DL-PCBs). Herein, ITOPSIS-IVIKOR-GRA incorporating distance and correlation by combining grey distance, grey group utility and individual regret, was developed to prioritize the risks of four typical recycled paper production by taking potential/actual health and ecological risks as criteria.

Alteration of intestinal microbiota-intestinal barrier interaction interferes with intestinal health after microcystin-LR exposure in Lithobates catesbeianus tadpoles.

There remains uncertainty regarding the influence of microcystin-leucine arginine (MC-LR) on amphibian intestinal health, specifically how MC-LR interferes with intestinal microbiota following exposure to environmental concentrations. In this study, Lithobates catesbeianus tadpoles were exposed to varying MC-LR concentrations (0, 0.5, and 2 µg/L) over a 30-day period.

Enhancing Miscanthus floridulus remediation of soil cadmium using Beauveria bassiana FE14: Plant growth promotion and microbial interactions.

Soil heavy metal pollution presents substantial risks to food security and human health. This study focused on the efficiency of plant growth-promoting fungus-Beauveria bassiana FE14 and Miscanthus floridulus on the synergistic remediation of soil Cd contamination. Results revealed that B.

Crucial role of the cGAS N terminus in mediating flowable and functional cGAS-DNA condensate formation via DNA interactions.

The DNA-sensing protein cGAS plays a pivotal role in the innate immune response and pathogenesis of various diseases. DNA triggers liquid-liquid phase separation (LLPS) and enhances the enzymatic activity of cGAS. However, the regulatory mechanisms of the disordered N terminus remain unclear.

Organocatalytic Activation of Alkynes Enabled Remote Control of Atroposelectivity via Vinylidene -Quinone Methides.

Axially chiral -VQMs have been extensively investigated as key intermediates to approach miscellaneous chiral structures. By sharp contrast, their structural isomers -VQMs have not been previously documented. The major reason, which results in the significant delay, may ascribe to the inherent challenges in the enantioselective activation of alkynes in a remote manner.

Development of a Deep Learning-Based Model for Pressure Injury Surface Assessment.

Aim: To develop a deep learning-based smart assessment model for pressure injury surface.

Design: Exploratory analysis study.

Methods: Pressure injury images from four Guangzhou hospitals were labelled and used to train a neural network model.

The molecular mechanism of temperature-dependent phase separation of heat shock factor 1.

Heat shock factor 1 (HSF1) is the critical orchestrator of cell responses to heat shock, and its dysfunction is linked to various diseases. HSF1 undergoes phase separation upon heat shock, and its activity is regulated by post-translational modifications (PTMs). The molecular details underlying HSF1 phase separation, temperature sensing and PTM regulation remain poorly understood.

Significant NO Formation in Truck Exhaust Plumes and Its Association with Ambient O: Evidence from Extensive Plume-Chasing Measurements.

Vehicle nitrogen oxides (NO) significantly increase nitrogen dioxide (NO) exposure in traffic-related environments. The NO/NO ratios are crucial for accurate NO modeling and are closely linked to public health concerns. In 2020, we used a mobile platform to follow test trucks (plume-chasing) that were installed with a portable emission measuring system (PEMS) on two restricted driving tracts.

Extracellular vesicles promote the infection and pathogenicity of Japanese encephalitis virus.

Japanese encephalitis virus (JEV) is a neurotropic zoonotic pathogen that poses a serious threat to public health. Currently, there is no specific therapeutic agent available for JEV infection, primarily due to the complexity of its infection mechanism and pathogenesis. Extracellular vesicles (EVs) have been known to play an important role in viral infection, but their specific functions in JEV infection remain unknown.

Deciphering progressive lesion areas in breast cancer spatial transcriptomics via TGR-NMF.

Identifying spatial domains is critical for understanding breast cancer tissue heterogeneity and providing insights into tumor progression. However, dropout events introduces computational challenges and the lack of transparency in methods such as graph neural networks limits their interpretability. This study aimed to decipher disease progression-related spatial domains in breast cancer spatial transcriptomics by developing the three graph regularized non-negative matrix factorization (TGR-NMF).

tRNA thiolation optimizes appressorium-mediated infection by enhancing codon-specific translation in Magnaporthe oryzae.

Thiolation, a post-transcriptional modification catalyzed by Uba4-Urm1-Ncs2/Ncs6 pathway in three specific transfer RNAs (tRNAs), is conserved from yeast to humans and plays an important role in enhancing codon-anticodon interaction and translation efficiency. Yet, except for affecting effector secretion, its roles in plant pathogenic fungi are not fully understood. Here, we used Magnaporthe oryzae as a model system to illustrate the vital role of s2U34 modification on the appressorium-mediated virulence.

The hepatic clock synergizes with HIF-1α to regulate nucleotide availability during liver damage repair.

Nucleotide availability is crucial for DNA replication and repair; however, the coordinating mechanisms in vivo remain unclear. Here, we show that the circadian clock in the liver controls the activity of the pentose phosphate pathway (PPP) to support de novo nucleotide biosynthesis for DNA synthesis demands. We demonstrate that disrupting the hepatic clock by genetic manipulation or mistimed feeding impairs PPP activity in male mice, leading to nucleotide imbalance.

Key bacteria decomposing animal and plant detritus in deep sea revealed via long-term incubation in different oceanic areas.

Transport of organic matter (OM) occurs widely in the form of animal and plant detritus in global oceans, playing a crucial role in global carbon cycling. While wood- and whale-falls have been extensively studied, the process of OM remineralization by microorganisms remains poorly understood particularly in pelagic regions on a global scale. Here, enrichment experiments with animal tissue or plant detritus were carried out in three deep seas for 4-12 months using the deep-sea incubators.

Klp2-mediated Rsp1-Mto1 colocalization inhibits microtubule-dependent microtubule assembly in fission yeast.

Microtubule assembly takes place at the centrosome and noncentrosomal microtubule-organizing centers (MTOCs). However, the mechanisms controlling the activity of noncentrosomal MTOCs are poorly understood. Here, using the fission yeast as a model organism, we demonstrate that the kinesin-14 motor Klp2 interacts with the J-domain Hsp70/Ssa1 cochaperone Rsp1, an inhibitory factor of microtubule assembly, and that Klp2 is required for the proper localization of Rsp1 to microtubules.

Nanomorphogenesis of interlayered polyamide membranes for precise ion sieving in lithium extraction.

Nanofiltration (NF) offers a scalable and energy-efficient method for lithium extraction from salt lakes. However, the selective separation of lithium from magnesium, particularly in brines with high magnesium concentrations, remains a significant challenge due to the close similarity in their hydrated ionic radii. The limited Li/Mgselectivity of current NF membranes is primarily attributed to insufficient control over pore size and surface charge.

Efficacy and safety of aspirin in preventing venous thromboembolism after hip arthroplasty for femoral neck fracture: a noninferiority prospective cohort study.

Background: Venous thromboembolism (VTE) is a common complication after hip arthroplasty. Here, we investigated the clinical efficacy and safety of prophylactic aspirin vs. conventional therapy in hip arthroplasty for femoral neck fracture.